Abstract:

【Objective】 The genetic background of 2 NILs, Brock/015//Jing4117 and Brock×Jing4117, which was to be thought resistant to powdery mildew, were evaluated with molecular detecting and the aim of this work was for breeding a good NILs and for using in wheat disease resistance mechanism studing and marker-assisted seletion breeding. 【Method】 AFLP technique was used in evaluation on genetic background of NILs, Brock and Jing411. Linkage relationships between Brock resistance gene and AFLP characteristic bands were also analyzed in Brock×Jing411 F2 segregation populations. 【Result】 Four types of AFLP band patterns, “parental ditype”, “tendency donor parent Brock type”, “tendency recurrent parent type Jing411”, and “crossingover type”, were identified in NILs. P15/M14-160 was identified in Brock, NILs and Brock×Jing411 F2 segregation resistance individuals. This AFLP band was not found in recurrent parent Jing411 and susceptible individuals. 【Conclusion】 The genetic background of 2 NILs was obtained and good genetic consistency was identified through comparion of NILs and donor parent Brock, recurrent parent Jing411. The results indicated that AFLP molecular detecting method can be used in genetic background evaluation of NIL. AFLP marker P15/m14-160 was highly linked to resistance gene for the powdery mildew in Brock. This study laied a foundation for wheat resistance powdery mildew marker- assited selection breeding.

Key words: wheat, near-isogenic lines, powdery mildew, AFLP