Abstract:

【Objective】 Dietary intake of isoflavones has been shown to reduce the risk of several major diseases in humans. Therefore, breeding soybean [Glycine max (L.) Merrill] seeds with desirable isoflavone content would be beneficial to the food and healthy industries, but the environmental sensitivity of the trait complicates phenotypic selection. The objective of this study was to identify quantitative trait loci (QTL) and epistatic interactions associated with isoflavone contents, protein contents, oil contents in soybean seeds. 【Method】 An F13 recombinant inbred line(RIL) comprising 474 lines was derived from a cross between Jindou23 and Huibuzhi. SSR technique was employed for mapping the QTLs. 【Result】 The QTLs for isoflavone content, protein content, oil content were analyzed and 23 QTLs were detected based on the constructed linkage map. Six QTLs for isoflavone content were localized in linkage groups J, N, D2, and G; Eleven QTLs for oil content were localized in the linkage groups A1, A2, B2, C2 and D2. Six QTLs for protein content were localized in linkage groups B2, C2, G and H1. The correlation analysis demonstrated that the content of isoflavone was in significant correlation with protein content. Significantly negative correlations were existed between oil content and protein content, and significantly positive correlations were existed between protein content and the content of total protein and oil. 【Conclusion】 All these have laid an important basis for the marker assisted breeding of the soybean. According to analysis of this investigation, the phenotype correlations of quantitative traits may result from the correlations of QTL controlling those traits.

Key words: soybean, SSR, QTL, quality, isoflavone