Abstract:

【Objective】 Maize dwarf mosaic disease caused by sugarcane mosaic virus (SCMV) is one of the important virus diseases in China and European countries. Marker development and markers-assisted selection (MAS) is an efficient way for disease-resistant breeding. 【Method】Resistant and susceptible DNA bulks were composed by using DNAs of 10 resistant and 12 susceptible inbred lines, respectively. Polymorphic AFLP markers were screened between two bulks and then transformed into SCAR (sequence characterized amplified region) markers. These SCAR markers associated with SCMV resistance was analyzed with disease incidence of 100 inbred lines. 【Result】 Two polymorphic AFLP markers, P66M38-220 and P55M51-240, were identified, and P66M38-220 was transformed into SCAR112, which was validated to be highly associated with SCMV resistance. 【Conclusion】 Improved BSA method is an efficient way for developing markers tightly linked with target traits. The SCAR112 developed in this study could be used for MAS of sugarcane mosaic virus resistance in maize.

Key words: maize, sugarcane mosaic virus, improved BSA method, AFLP marker, SCAR marker