Abstract: 【OBJECTIVE】Lmbr1 is the key candidate gene underlying human and mouse preaxial polydactyly(PPD), the mapping result of chicken polydactyl and comparative genomic analysis of polydacytlous traits among other species shows that lmbr1 should be the potential critical candidate gene of chicken PPD;【METHOD】Here we conduct the cloning of chicken lmbr1 intron 13, the Single Nucleotide Polymorphisms(SNPs) analysis, the sequence conservation among several species ,the halpotype distribution and the association of gene polymorphisms with toe phenotype with direct sequencing and PCR-SSCP methods in resource population established by polydactlous silkies and four-toed White Plymouth Rock broiler ;【RESULTS】4 SNPs were detected from Silkies and White Plymouth Rock from these region；and it was found that PCR-SSCP genotypes of intron 13 were significantly associated with chicken toe phenotype;【CONCLUSION】 it shows that the variations of lmbr1 intron 13 were tightly linked with the special site underlying chicken polydactlous mutant ,which means that lmbr1 should be the key candidate gene of chicken polydactyl, so the next step work should be to further detect the gene polymorphisms in whole genomic level of lmbr1 and its surrounding genes .

Key words: lmbr1, chicken, intron, SNPs, polydactyl, association