Scientia Agricultura Sinica ›› 2005, Vol. 38 ›› Issue (03): 511-515 .

• PLANT PROTECTION • Previous Articles     Next Articles

Synthesis and Identification of Artificial Antigen for Imidacloprid

,,,   

  1. 浙江大学农药与环境毒理研究所
  • Received:2003-12-12 Revised:1900-01-01 Online:2005-03-10 Published:2005-03-10

PDF

944

Abstract: This study reported that a hapten of imidacloprid, 1-[(6-Carboxylethylthio-3-pyridinyl)methyl]-N-nitro- imidazolidinimine was synthesized by using technical grade imidacloprid reacted withβ-Mercaptopropionic acid in the hot alkalic solution. The hapten was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to form the artificial antigens, respectively. The antibody with high titer (2.56×104) was produced after immuning to rabbits. Results showed that the antibody hadhigh affinity to imidacloprid tested by enzyme linked immunosorbent assay (ELISA), which IC50 and IC20 were 20.7 ?g·L-1 and 1.1 ?g·L-1 ; and the crosss-reactibities to the related compounds wereless than 1.4%.

Key words: Imidacloprid, Artificial antigen, Antibody

[1] QIU YiLei,WU Fan,ZHANG Li,LI HongLiang. Effects of Sublethal Doses of Imidacloprid on the Expression of Neurometabolic Genes in Apis cerana cerana [J]. Scientia Agricultura Sinica, 2022, 55(8): 1685-1694.
[2] LI YaFei, SHI JiangLan, WU TianQi, WANG ShaoXia, LI YuNuo, QU ChunYan, LIU CongHui, NING Peng, TIAN XiaoHong. Effects of Combined Foliar Application of Zinc with Imidacloprid on Zinc Enrichment and Protein Components Content in Wheat Grain [J]. Scientia Agricultura Sinica, 2022, 55(3): 514-528.
[3] LI ZhiLing,LI XiangJu,CUI HaiLan,YU HaiYan,CHEN JingChao. Development and Application of ELISA Kit for Detection of EPSPS in Eleusine indica [J]. Scientia Agricultura Sinica, 2022, 55(24): 4851-4862.
[4] ZHANG Rui,ZHANG XueYao,ZHAO XiaoMing,MA EnBo,ZHANG JianZhen. Antibody Preparation and Subcellular Localization of LmKnk3-5′ in Locusta migratoria [J]. Scientia Agricultura Sinica, 2022, 55(2): 329-338.
[5] ZHANG FengXi,XIAO Qi,ZHU JiaPing,YIN LiHong,ZHAO XiaLing,YAN MingShuai,XU JinHua,WEN LiBin,NIU JiaQiang,HE KongWang. Preparation and Identification of Monoclonal Antibodies to P30 Protein and Establishment of Blocking ELISA to Detecting Antibodies Against African Swine Fever Virus [J]. Scientia Agricultura Sinica, 2022, 55(16): 3256-3266.
[6] WEI Tian,WANG ChengYu,WANG FengJie,LI ZhongPeng,ZHANG FangYu,ZHANG ShouFeng,HU RongLiang,LÜ LiLiang,WANG YongZhi. Preparation of Monoclonal Antibodies Against the p30 Protein of African Swine Fever Virus and Its Mapping of Linear Epitopes [J]. Scientia Agricultura Sinica, 2022, 55(15): 3062-3070.
[7] YuXin LIANG,JianXiang WU,XiaoYu LI,ChunYu ZHANG,JiChao HOU,XuePing ZHOU,YongZhi WANG. Mapping of Epitopes and Establishment of Rapid DAS-ELISA for Potato Virus Y Coat Protein [J]. Scientia Agricultura Sinica, 2021, 54(6): 1154-1162.
[8] LI MinXue,LI JianNan,ZHOU Hong,XIAO Ning,LIN HuiXing,MA Zhe,FAN HongJie. Establishment and Preliminary Application of Lawsonia intracellularis IPMA Antigen Detection Method Based on SodC Monoclonal Antibody [J]. Scientia Agricultura Sinica, 2021, 54(20): 4478-4486.
[9] TAN YongAn,ZHAO XuDong,JIANG YiPing,ZHAO Jing,XIAO LiuBin,HAO DeJun. Cloning, Preparation of Antibody and Response Induced by 20-Hydroxyecdysone of Target of Rapamycin in Apolygus lucorum [J]. Scientia Agricultura Sinica, 2021, 54(10): 2118-2131.
[10] HU XiaoFei,LI QingMei,YAO JingJing,HU SiYu,SUN YaNing,XING YunRui,DENG RuiGuang,ZHANG GaiPing. Development of High Sensitive Zeranol Monoclonal Antibody Based on the Cross Reactivity of Structural Analogs [J]. Scientia Agricultura Sinica, 2020, 53(5): 1071-1080.
[11] WANG XiaoLei,LIN Jian,YANG ZhiYuan,LIU LiXin,DUAN HuiJuan,CHENG HuiMin,ZHAO JiCheng,PAN Jie,LIU YueHuan. Correlation Research of the Serological Test (HI) and Challenge Test of Duck Tembusu Virus Inactivated Vaccine [J]. Scientia Agricultura Sinica, 2019, 52(23): 4423-4428.
[12] BI KeRan,LI Yin,HAN KaiKai,ZHAO DongMin,LIU QingTao,LIU YuZhuo,HUANG XinMei,YANG Jing. Prokaryotic Expression and Polyclonal Antibody Preparation of Duck Oligoadenylate Synthase-Like Protein [J]. Scientia Agricultura Sinica, 2019, 52(23): 4429-4436.
[13] ZHANG Kui, PAN GuangZhao, SU JingJing, TAN Juan, XU Man, LI YuTian, CUI HongJuan. Identification, Expression, Subcelluar Localization, and Function of glial cell missing (gcm) in Silkworm (Bombyx mori) [J]. Scientia Agricultura Sinica, 2018, 51(7): 1401-1411.
[14] SONG HuiFang, ZHANG JianQin, FAN YunHe, LI Tao, MA EnBo, ZHANG JianZhen. Antibody Preparation and Subcelluar Localization of dsRNA Degrading Enzyme in Locusta migratoria [J]. Scientia Agricultura Sinica, 2018, 51(19): 3704-3713.
[15] ZHANG TingTing, LIU WeiWei, GAO Lu, LI RenJian, FU SuiYe, LIU XiaoJian, LI DaQi, LIU WeiMin, DONG Qing, ZHANG JianZhen. The antibody preparation and expression analysis of Chitinase 5-1 in Locusta migratoria [J]. Scientia Agricultura Sinica, 2018, 51(12): 2418-2428.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd