Scientia Agricultura Sinica ›› 2004, Vol. 37 ›› Issue (10): 1575-1579 .

• RESEARCH NOTES • Previous Articles     Next Articles

Development of an Enzyme Immunoassay for the Determination of the Cowpea Trypsin Inhibitor (CpTI) in Transgenic Crop

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  1. 中国农业大学作物化学控制中心
  • Received:2002-11-05 Revised:2003-11-20 Online:2004-10-20 Published:2004-10-20

Abstract: CpTI was purified by G-75 column and affinity chromatography from 901 QINGPI cowpea which was selected from several varieties by trypsin inhibitor activity. The polyclonal antibody against CpTI was prepared and an enzyme immunoassay was established. Several samples of transgenic CpTI cotton were determined. The results showed that the expression of different organs of the same cultivar was different.

Key words: Cowpea trypsin inhibitor (CpTI), Enzyme immunoassay, Transgenic crop

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