Scientia Agricultura Sinica ›› 2006, Vol. 39 ›› Issue (06): 1146-1152 .doi: 10.3864/j.issn.0578-1752.at-2005-5947

• PLANT PROTECTION • Previous Articles     Next Articles

The Molecular Tagging of theYellow Rust Resistance Gene Yr7 in Wheat Transferred from Differential Host Lee Using Microsatellite Markers

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  • Received:2005-08-31 Revised:1900-01-01 Online:2006-06-10 Published:2006-06-10

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Abstract: 【Objective】Using inheritance analysis for the rust reaction of seedling plants in Taichung29*6/Lee to CYR27 and SSR for tagging the resistance gene in Taichung29*6/Yrlee,made from the donor parent Lee and recurrent parent Taichung29.【Method】Using an assessment of the rust reaction of seedling plants in the F1, F2 and BC1 population for a set of crosses made between Lee, Taichung29 and Taichung29*6/Lee.According to WMS and WMC SSR primer sets in number of 88 distributed among 2B, 22 among 4D and 6D chromosomes, the PCR amplification, denatured PAGE and silver staining are used for detecting polymorphic bands.【Result】The proportions of resistant and susceptible plants obtained following inoculation of F2 populations with race CYR27 indicates that single dominant gene determines the reactions of Taichung29*6/Lee lines. For 22 primer sets distributed among 4D and 6D chromosomes, polymorphic bands are not identified between Taichung29*6/Yrlee and Taichung29. The primer pair Xgwms526 on 2B in number of 88 primer sets have two SSR amplified polymorphic bands Xgwms526/212 and Xgwms526/216 detected. Further screening the F2 resistant and susceptible individuals , it showed that Xgwm526 co-segregated with the resistant. gene in wheat and confirmed that the gene in Taichung29*6/Yrlee line is Yr7. The individuals of segregated F2 population are amplified using diagnostic primer Xgwm526, there are 22 A bands, 51 H bands and 2 B bands in 75 resistant individuals, while 27 B bands , 4 H bands in 31 susceptible individuals.【Conclusion】The results of software Map Manager QTX 17b analysis shows that the genetic distance between Xgwm526 and Yr7 was as 5.3 cM , LOD=18.4. Furthermore the marker Xgwm526 can be used for the detection of resistance gene Yr7 and for the pyramiding with other yellow rust resistance genes in wheat shows that the genetic distance between Xgwm526 and Yr7 was as 5.3 cM , LOD=18.4. Furthermore the marker Xgwm526 can be used for the detection of resistance gene Yr7 and for the pyramiding with other yellow rust resistance genes in wheat.

Key words: Wheat, Puccinia striiformis f.sp.tritic, Differential host, Lee, Resistance gene, Microsatellite marker

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