Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (9): 1932-1938.doi: 10.3864/j.issn.0578-1752.2013.09.021

• VETERINARY SCIENCE • Previous Articles     Next Articles

Effects of Prion Protein on the Regulation of Classical and Alternative Activation of BV2 Microglia in vitro

 FU  Yong-Yao, SHI  Fu-Shan, WANG  Ji-Hong, YANG  Li-Feng, ZHOU  Xiang-Mei, YIN  Xiao-Min, ZHAO  De-Ming   

  1. Laboratory of National Animal TSE/College of Veterinary Medicine, China Agricultural University, Beijing 100193
  • Received:2012-01-07 Online:2013-05-01 Published:2013-03-30

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Abstract: 【Objective】 The purpose of this study is to investigate the effects of PrPC on various forms of microglial activation. 【Method】 BV2 microglia were treated, respectively, with IFN-γ, IL-4, or IL-10, and the mRNA expression of PRNP was examined by RT-PCR. Then the effects of si-RNA-mediated disruption of PRNP on different parameters of microglial activation in IFN-γ, IL-4, or IL-10-stimulated microglia were analyzed by RT-PCR and western-blot. 【Result】PRNP mRNA expression was invariably downregulated in microglia upon exposure to IFN-γ, IL-4, or IL-10. PRNP silencing prior to cytokines treatment reduced the responsiveness of microglia to INF-γ treatment, significantly altered IL-4-induced microglial activation phenotype, and had no effect on IL-10-induced microglial activation.【Conclusion】Together, these results support a role of PrPC in the modulation of the shift of microglia from a quiescent state to an activated phenotype and in the regulation of the microglial response during classical and alternative activation.

Key words: SiRNA , prion protein , microglia , activation

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