Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (3): 507-516.doi: 10.3864/j.issn.0578-1752.2013.03.007

• PLANT PROTECTION • Previous Articles     Next Articles

Effects of Application of Anaerobically Digested Slurry on Suppression of Pepper (Capsicum frutescens L.) Blight and Soil Biological Characteristics

 CAO  Yun, CHANG  Zhi-Zhou, MA  Yan, YANG  Hao, FU  Guang-Qing   

  1. Jiangsu Agricultural Waste Treatment and Recycle Engineering Research Center, Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014
  • Received:2012-08-15 Online:2013-02-01 Published:2012-10-11

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Abstract: 【Objective】 The objective of this study is to evaluate the effects of application of anaerobically digested slurry (ADS) on reduction of the incidence of Phytophthora blight of chilli pepper and soil biological characteristics. The potential mechanisms of plant disease suppression by ADS application was also proposed. 【Method】 Three consecutive batches of pot experiment were carried out to study the control of Phytophthora blight of chilli pepper by substitution of 50% N input with raw pig slurry, raw dairy slurry, anaerobically digested pig and dairy slurry. The variations in activities of soil enzymes were analyzed. And the changes in soil microbial diversities and microbial structures were studied by PCR-DGGE.【Result】Application of anaerobically digested pig slurry resulted in the best control efficacy against pepper blight and the disease incidence rate was decreased by 79.3%, followed by combined application of anaerobically digested dairy slurry with chemical fertilizer (38.5% of control efficacy). Application of raw pig and dairy slurry had no significant suppressive effect on pepper blight. Plants treated by anaerobically digested pig and dairy slurry showed significantly higher shoot biomass of pepper plants than those treated by corresponding slurry and negative control. The fluorescein diacetate (FDA) hydrolase activity, catalase activity and polyphenol oxidase activity of soil treated by anaerobically digested pig slurry were 3.44-fold, 66.7% and 76.7% higher than those of positive control, respectively. In addition, application of ADS significantly increased the numbers of soil bacteria, fungi, actinomycete, and antagonistic microorganisms including Pseudomonas fluorescents and Tricoderma spp.. The PCR-DGGE analysis showed that the bacterial and fungal diversities in the ADS treatments were improved. 【Conclusion】 The combined application of anaerobically digested pig slurry with chemical fertilizer obtained great achievement in reducing pepper blight incidence and had immense potential in agricultural production. Regulation of microbial diversity in rhizosphere and improvement of soil fertility might be one of the possible mechanisms on disease suppression of pepper blight by ADS application.

Key words: anaerobically digested slurry , soil biological characteristics , pepper blight , animal manure , microflora

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