Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (23): 4854-4861.doi: 10.3864/j.issn.0578-1752.2012.23.012

• HORTICULTURE • Previous Articles     Next Articles

Difference Analysis on the Degree of Positive and Negative Hybridization Affinity for Asian Lily and Longiflorum Lily

 DU  Wen-Wen, WANG  Xiang-Ning, WU  Li-Fang, DUAN  Qing, CUI  Guang-Fen, WANG  Ji-Hua   

  1. 1.College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070
    2. Yunnan Key Laboratory for Flower Breeding/Flower Institute of Yunnan Agricultural Science Academy, Kunming 650205
  • Received:2012-07-18 Online:2012-12-01 Published:2012-09-26

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Abstract: 【Objective】 The objective of this study was to study hybridization affinity of Asian lily and Longiflorum lily, and compare affinity differences existing in the obverse and inverse crosses.【Method】Asian lily ‘Brunello’ and Longiflorum lily ‘Formolongi Ⅱ’ were used as the research materials and their reciprocal crosses were selected. The dynamic growth of the pollen in the pistil was observed by fluorescence microscopy. 【Result】 Pollen grains of the both cross combinations were able to germinate on the stigma. But, pollen tubes successively appeared callose reaction in the stigma papillae and style channel cells. Within 7-18 h after hybridization, the average growth rate of pollen tubes of cross combination was faster than the reciprocal cross combination. After 18h, the growth rate of pollen tubes of negative cross gradually accelerated and exceeded the positive cross. In the cross combination, pollen tubes reached half of style and stopped growth at 55 h after pollination. The pollen tubes of the reciprocal cross combination entered ovary at 48 h after pollination. The cross and reciprocal cross both showed hybrid incompatibility that occurred in stigma and style. 【Conclusion】 The extent of incompatibility in the reciprocal crosses had differences. Cutting style pollination, ovary pollination and embryo culture were able to overcome hybridization barriers and improve probability of hybrid embryos.

Key words: lily , distant hybridization , fluorescence microscopy , pollen tubes , callose

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