基于SCAR标记的小麦禾谷孢囊线虫快速分子检测技术

doi:10.3864/j.issn.0578-1752.2012.21.007

Abstract

Abstract: 【Objective】 The objective of this study is to build a SCAR marker system for rapid molecular detection of cereal cyst nematode (Heterodera avenae) from mixtures of different nematodes. 【Method】 With the method of RAPD and SCAR, a single-step PCR was used to specially detect H. avenae. 【Result】 Using the SCAR marker system established in this study, the target fragment was amplified from the DNA templates of H. avenae only, from other 8 species including 32 populations. The SCAR marker system could detect cysts and the J2 of H. avenae, and the specific fragment could be clearly identified when the dilution was 1/2000 of a cyst or 1/80 of a J2 for all replicates. 【Conclusion】 The establishment of the SCAR marker system for rapid molecular detection of H. avenae can be used to detect the H. avenae from the mixed nematode samples rapidly, and the detection is accurate and highly sensitive.

Key words: Heterodera avenae, SCAR marker, rapid molecular diagnosis

QI Xiao-Li, PENG De-Liang, PENG Huan, LONG Hai-Bo, HUANG Wen-Kun, HE Wen-Ting. Rapid Molecular Diagnosis Based on SCAR Marker System for Cereal Cyst Nematode[J].Scientia Agricultura Sinica, 2012, 45(21): 4388-4395.

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Rapid Molecular Diagnosis Based on SCAR Marker System for Cereal Cyst Nematode

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