Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3288-3296.doi: 10.3864/j.issn.0578-1752.2012.16.008

• PLANT PROTECTION • Previous Articles     Next Articles

Construction of Recombinant BmNPV Infecting Ecotropis oblique

 GUO  Rui, LIU  Wei-Xing, PAN  Zhong-Hua, XUE  Ren-Yu, CAO  Guang-Li, ZHU  Yue-Xiong, GONG  Cheng-Liang   

  1. 苏州大学基础医学与生物科学学院，江苏苏州 215123
  • Received:2011-11-14 Online:2012-08-15 Published:2012-01-13

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Abstract: 【Objective】The objective of this study is to construct recombinant BmNPV infecting both silkworm and Ecotropis oblique, and to solve the reproduction difficulty of EoNPV because of the breeding complexity.【Method】Recombinant BmNPV containing the polh expression box of BmNPV and the hel expression box of EoNPV were constructed by Bac-to-Bac system. 【Result】rBmNPV amplified from silkworm or BmN cell could infect E. oblique, which indicated the host domain of rBmNPV had been broaden to E. oblique. 【Conclusion】rBmNPV which could infect E. oblique was successfully constructed, thus laied a foundation of producing recombinant virus pesticide using silkworm as host.    

Key words: EoNPV, BmNPV, recombinant virus, host domain

[1]胡  萃. 茶尺蠖. 上海: 上海科学技术出版社, 1994.

Hu C. Ecotropis obliqua. Shanghai: Shanghai Science and Technology Press, 1994. (in Chinese)

[2]侯建文, 赵烨烽. 茶尺蠖的天敌及其控制作用. 茶叶, 1988(1): 15-18.

Hou J W, Zhao Y F. The natural enemy of Ecotropis oblique and its regulation. Tea, 1988(1): 15-18. (in Chinese)

[3]潘亚飞, 赵敬钊. 斜纹猫蛛对茶尺蠖幼虫捕食作用的初步研究. 蛛形学报, 1996, 5(2): 149-153.

Pan Y F, Zhao J T. Study on predation efficiency of Oxyopes sertatus to the larvae of Ectropis obliqua hypulina. Acta Arachnologica Sinica, 1996, 5(2): 149-153. (in Chinese)

[4]胡  萃, 叶恭银, 林高明, 吴伟龙, 朱俊庆, 商建农. 茶尺蠖NPV对茶尺蠖幼虫取食及生长发育的影响. 浙江农业大学学报, 1990, 16(2): 113-118.

Hu C, Ye G Y, Lin G M, Wu W L, Zhu J Q, Shang J N. Effect of the nuclear polyhedrosis virus from tea geometrid (Ectropis obliqua hypulina) on feeding, growth and development of tea geometrid larvae. Acta Agriculturae Universitatis Zhejiangensis, 1990, 16(2): 113-l18. (in Chinese)

[5]张益民, 王学兰, 张世敏, 袁  丽, 洪北边, 殷坤山. 茶尺蠖核型多角体病毒超微结构的初步研究. 科学通报, 1985(24): 1919-1920.

Zhang Y M, Wang X L, Zhang S M, Yuan L, Hong B B, Yin K S. Preliminary inquiry of Ecotropis obliqua nucleopolyhedrovirus’ ultrastructure. Chinese Science Bulletin, 1985(24): 1919-1920. (in Chinese)

[6]陈历程. NPV对不同龄期茶尺蠖的敏感性. 福建茶叶, 1988(2): 38-39.

Chen L C. The sensibility of NPV to Ecotropis obliqua in different instar. Tea in Fujian, 1988(2): 38-39. (in Chinese)

[7]吴晓晶, 胡  萃. 茶尺蠖核型多角体病毒的大量增殖研究. 浙江农业大学学报, 1987, 13(2): 150-157.

Wu X J, Hu C. Study on the mass production of the nuclear polyhedrosis virus of the tea geometrid , Ectropis obliqua hypulina Wehrli. Acta Agriculturae Universitatis Zhejiangensis, 1987, 13(2): 150-157. (in Chinese)

[8]叶恭银, 胡  萃. 温度对茶尺蠖核型多角体病毒增殖动态的影响. 病毒学杂志, 1990(4): 397-403.

Ye G Y, Hu C. Effect of temperature on the growth dynamics of the nuclear polyhedrosis virus from Ectropis oblique hypulina Wehrli in its host larvae. Virologica Sinica, 1990(4): 397-403. (in Chinese)

[9]Ye G Y, Hu C, Zhu J Q, Shang J N. Effect of the nuclear polyhedrosis from tea geometrid (Eotropis obiqua) on its host feeding, growth, development, reproduction and populational dynamics// Proceeding of the XIX International Congress of Entomology. Beijing, China. 1992: 280.

[10]Zhang C X, Hu C. Nucleotide sequence of the polyhedron gene of Ectropis obliqua single enveloped nucleopolyhedrovirus and the placement of the virus in baculoviridae. Chinese Journal of Biochemistry and Molecular Biology, 1999, 15(3): 365-371.

[11]叶恭银, 胡  萃. 三种主要环境因子对茶尺蠖核型多角体病毒毒力的影响. 应用生态学报, 1991, 2(3): 269-274.

Ye G Y, Hu C. Effects of three main environmental factors on virulence of NPV from tea geometrid. Chinese Journal of Applied Ecology, 1991, 2(3): 269-274. (in Chinese)

[12]殷坤山, 肖  强, 唐美君, 郭华伟, 黄巨永. 温度、光、pH值对茶尺蠖病毒活性的影响. 茶叶, 2004, 30(2): 85-87.

Yin K S, Xiao Q, Tang M J, Guo H W, Huang J Y. Effect of temperature, light and pH on activity of nuclear polyhedrosis virus of Ectropis obliqua (EoNPV). Journal of Tea, 2004, 30(2): 85-87. (in Chinese)

[13]殷坤山, 洪北边. 茶尺蠖核型多角体病毒的土法生产. 中国茶叶, 1988(3): 4-6.

Yin K S, Hong B B. Indigenous mthod of Ecotropis obliqua nucleopolyhedrovirus. China Tea, 1988(3): 4-6. (in Chinese)

[14]殷坤山, 陈华才, 唐美君, 郭华伟, 肖  强, 姚惠明. 茶尺蠖病毒杀虫剂田间使用技术的研究. 中国病毒学, 2003, 18(5): 492-495.

Yin K S, Chen H C, Tang M J, Guo H W, Xiao Q, Yao H M. Studies on application techniques of EoNPV preparations. Virologica Sinica, 2003, 18(5): 492-495. (in Chinese)

[15]刘同先, 康  乐. 昆虫学研究进展与展望. 北京: 科学出版社, 2005: 76-85.

Liu T X, Kang L. The Development and Prospect of Insectology. Beijing: Science Press, 2005: 76-85. (in Chinese)

[16]Sun X, Chen X, Zhang Z, Wang H, Bianchi F J, Peng H, Vlak J M, Hu Z. Bollworm responses to release of genetically modified Helicoverpa armigera nucleopolyhedroviruses in cotton. Journal of Invertebrate Pathology, 2002, 81(2): 63-69.

[17]李洪波, 夏玉先. 昆虫神经毒素LqhIT2的表达、抗血清制备及活性分析. 生物工程学报, 2008, 24(10): 1761-1767.

Li H B, Xia Y X. Expression, antiserum preparation and bioactivity assays of insect neurotoxin LqhIT2. Chinese Journal of Biotechnology, 2008, 24(10): 1761-1767. (in Chinese)

[18]Regev A, Rivkin H, Inceoglu B, Gershburq E, Hammock B D, Gurevitz M, Chejanovsky N. Further enhancement of baculovirus insecticidal efficacy with scorpion toxins that interact cooperatively. FEBS Letters, 2003, 537(1/3): 106-110.

[19]Thiem S M, Du X, Quentin M E, Berner M M. Identification of a baculovirus gene that promotes Autographa californica nuclear polyhedrosis virus replication in a nonpermissive insect cell line. Journal of Virology, 1996, 70(4): 2221-2229.

[20]Thiem S M. Prospects for altering host range for baculovirus bioinsecticides. Current Opinion in Biotechnology, 1997, 8(3): 317-322.

[21]Kondo A, Maeda S. Host range expansion by recombination of the baculoviruses Bombyx mori nuclear polyhedrosis virus and Autographa californica nuclear polyhedrosis virus. Journal of Virology, 1991, 65(7): 3625-3632.

[22]Maeda S, Kamita S G, Kondo A. Host range expansion of Autographa californica nuclear polyhedrosis virus (NPV) following recombination of a 0.6 kilobase-pair DNA fragment originating from Bombyx mori NPV. Journal of Virology, 1993, 67(10): 6234-6238.

[23]Croizier G, Croizier L, Argaud O, Poudeviqne D. Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene. Proceedings of the National Academy of Sciences of the United States of America, 1994, 91(1): 48-52.

[24]易咏竹, 张志芳, 肖庆利, 何家禄. 宿主域扩大的苜蓿尺蠖核多角体杂交型病毒. 蚕业科学, 2001, 27(1): 75-76.

Yi Y Z, Zhang Z F, Xiao Q L, He J L. The host range expanded hybrid Autographa californica nuclear polyhedrosis virus. Acta Sericologica Sinica, 2001, 27(1): 75-76. (in Chinese)

[25]Wu X F, Cao C P, Xu Y X, Lu X M. Construction of a host range-expanded hybrid baculovirus of BmNPV and AcNPV, and knockout of cysteinase gene for more efficient expression. Science in China C: Life Sciences, 2004, 47(5): 406-415.

[26]Ishikawa H, Ikeda M, Alves C A F, Thiem S M, Kobayashi M. Host range factor 1 from Lymantria dispar nucleopolyhedrovirus (NPV) is an essential viral factor required for productive infection of NPVs in IPLB-Ld652Y cells derived from L. dispar. Journal of Virology, 2004, 78(22): 12703-12708.

[27]Ma X C, Xu H J, Tang M J, Xiao Q, Hong J, Zhang C X. Morphological, phylogenetic and biological characteristics of Ectropis obliqua single-nucleocapsid nucleopolyhedrovirus. The Journal of Microbiology, 2006, 44(1): 77-82.

[28]Ma X C, Shang J Y, Yang Z N, Bao Y Y, Xiao Q, Zhang C X. Genome seqence and organization of a nucleopolyhedrovirus that infects the tea looper caterpillar, Ectropis oblique. Virology, 2007, 360(1): 235-246.

[29]曹翠平, 兰丽盼, 姚慧鹏, 何芳青, 郭爱芹, 吴小锋. 家蚕Polh+ Bac-to-Bac杆状病毒表达系统的构建. 蚕业科学, 2008, 34(2): 237-243.

Cao C P, Lan L P, Yao H P, He F Q, Guo A Q, Wu X F. Construction of a BmNPV polyhedrin-plus bac-to-bac system for the silkworm, Bombyx mori. Acta Sericologica Sinica, 2008, 34(2): 237-243. (in Chinese)

[30]Summers M D, Smith G E. A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experimental Station Bulletin, 1987, 1555: 1-57.

[31]Bideshi D K, Federici B A. The Trichoplusia ni granulovirus helicase is unable to support replication of Autographa californica multicapsid nucleopolyhedrovirus in cells and larvae of T. ni. Journal of General Virology, 2000, 81(6): 1593-1599.
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