Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1881-1886.doi: 10.3864/j.issn.0578-1752.2012.09.025

• RESEARCH NOTES • Previous Articles    

Effects of Lactic Acid Bacteria on APRIL and IL-10 Secretion in Caco-2 Cells

 HUANG  Yi, HUANG  Qin, LI  Ya-Li, CUI  Zhi-Wen, LI  Wei-Fen, YU  Dong-You   

  1. 1.广西大学动物科学技术学院，南宁 530005
    2.浙江大学动物科学学院/教育部动物分子营养学重点实验室，杭州 310058
  • Received:2011-08-15 Online:2012-05-01 Published:2012-03-01

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Abstract: 【Objective】 The study was designed to evaluate the immunological effects of probiotic strains Enterococcus faecium and Lactococcus lactis on production of pro-inflammatory cytokine of APRIL and anti-inflammatory cytokine interleukin-10 in intestinal epithelial cells. 【Method】Human colon adenocarcinoma cell line, Caco-2 cells were incubated with PBS (Group CT, negative control), Escherichia coli K88 (Group EC, positive control), and Enterococcus faecium (Group EF), and Lactococcus lactis (Group LL) for 2 h, respectively. Besides, Caco-2 cells were firstly treated with E. faecium and L. lactis for 1 h, respectively, and then followed by incubation with E. coli K88 for an additional 2 h (Group EF-EC and Group LL-EC). Culture supernatants were collected for analyzing the contents of APRIL and IL-10 by ELISA method. 【Result】 Results showed that E. faecium and L. lactis induced an increased release of APRIL and IL-10. Pre-culture of Caco-2 cells with E. faecium and L. lactis was capable of markedly up-regulating production of IL-10 while decreasing APRIL secretion following co-culture with E. coli K88. 【Conclusion】These findings demonstrated that E. faecium and L. lactis could activate immunity of intestinal epithelial cells by inducing APRIL and IL-10 secretion. Moreover, these two strains of lactic acid bacteria also exhibited anti-inflammatory properties via modulating the immune response in Caco-2 cells when they were infected with E. coli K88.

Key words: Caco-2 cell, Enterococcus faecium, Lactococcus lactis, Escherichia coli K88, APRIL, IL-10, ELISA

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