Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (6): 1176-1182.doi: 10.3864/j.issn.0578-1752.2012.06.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Studies on Expression Characteristics of EGF and Development Characteristics of Hair Follicle of Gray Goat

 WANG  Rui-Zhi, YIN  Xun-He, WANG  Hui, CHENG  Zi-Qiang   

  1. 1.山东农业大学动物科技学院，山东泰安 271018
    2.山东农业大学动物科学国家级教学中心，山东泰安 271018
  • Received:2011-04-18 Online:2012-03-15 Published:2011-06-29

PDF

645

Cited

3

Abstract: 【Objective】 The relationship between the expression of epidermal growth factor and the development of hair follicle was studied.【Method】 The histology method, micro-observation approach and QRT-PCR were used to detect the relationship between the expression of EGF and the development of hair follicle of 1-day-old, 1-month-old, 2-month-old, 3-month-old, 4-month-old and 5-month-old Jining Gray goat. SAS9.0 was used to analyze the relationship between them.【Result】 The resuts indicated that the diameter of primary follicle increased, but not significantly (P＞0.05). The diameter of secondary follicle increased very significantly (P＜0.01). The diameter of secondary follicle reached peak of growth at 2-month-old. The expression of EGF increased after birth and achieved the highest at 2-month-old (P＜0.01). It decreased extremely significant after 2-month-old (P＜0.01).【Conclusion】 The primary follicle was completely developed before birth and the secondary follicle was developed after birth. The expression of EGF and the diameter of secondary follicle had a significant positive correlation before 2-month-old (P＜0.01). The results indicated that EGF is significantly positive to the development of secondary follicle.

Key words: Jining Gray goat, hair follicle development, epidermal growth factor, quantitative PCR

[1]Jahoda C A, Oliver R F, Reynolds A J, Forrester J C, Gillespie J W, Cserhalmi-Firedman P B, Christiano A M, Horne K A. Trans-species hair growth induction by human hair follicle dermal papillae. Experimental Dermatology, 2001, 10: 229-237.

[2]李长青, 尹  俊, 张燕军, 郭志成, 张文广, 高爱琴, 李玉荣, 赖双英, 李金泉. 内蒙古绒山羊与辽宁绒山羊皮肤毛囊周期性变化的比较研究. 畜牧兽医学报, 2005, 36(7): 674-679.

Li C Q, Yin J, Zhang Y J, Guo Z C, Zhang W G, Gao A Q, Li Y R, Lai S Y, Li J Q. Comparative study on skin and hair follicles cycling between Inner Mongolia and Liaoning Cashmere goats. Chinese Journal of Animal and Veterinary Sciences, 2005, 36(7): 674-679. (in Chinese)

[3]董  彬, 崔志峰, 尹逊河, 王  慧, 王树迎. 济宁青山羊皮肤毛囊的组织学特性研究. 山东农业大学学报: 自然科学版, 2010, 41(2): 258-262.

Dong B, Cui Z F, Yin X H, Wang H, Wang S Y. Studies on histological characteristics of Jining Gary goat skins and hair follicle. Journal of Shandong Agricultural University: Natural Science, 2010, 41(2): 258-262. (in Chinese)

[4]卿素珠, 林吉茂, 张灵枝, 王磊磊, 罗时双. 表皮生长因子及其受体在山羊胎儿皮肤发育中的表达特征. 畜牧兽医学报, 2009, 40(9): 1383-1388.

Qing S Z, Lin J M, Zhang L Z, Wang L L, Luo S S. Expression characteristics of EGF and EGFR in developing skin of goat fetus. Chinese Journal of Animal and Veterinary Sciences, 2009, 40(9): 1383-1388. (in Chinese)

[5]Stenn K S, Paus R. Controls of hair follicle cycling. Physiological Reviews, 2001, 81(1): 449-494.

[6]Kumamoto T, Shalhevet D, Matsue H, Mummert M E, Ward B R, Jester J V, Takashima A. Hair follicles serve as local reservoirs of skin mast cell precursors. Blood, 2003, 102(5): 1654-1660.

[7]李国强, 纪影畅, 李  宇. 毛囊形态发生的分子机制. 国外医学皮肤性病学分册, 2004, 30(1): 38-40.

Li G Q, Ji Y C, Li Y. Molecular mechanism of the development of hair follicle. Foreign Medical Sciences Section of Dermatology and Venereology, 2004, 30(1): 38-40. (in Chinese)

[8]叶  榕. 表皮生长因子受体及其临床应用. 医学新知杂志, 2008, 18(3): 164-166.

Ye R. Epidermal growth factor receptor and application. Journal of New Medicine, 2008, 18(3): 164-166. (in Chinese)

[9]Saga K, Jimbow K. Immunohistochemical localization of activated EGF receptor in human eccrine and apocrine sweat glands. The Journal of Histochemistry and Cytochemistry, 2001, 49(5): 597-601.

[10]周  岗, 付小兵, 陈  伟, 李海红, 姜笃银, 白晓东, 雷永红, 孙同柱. 胎儿皮肤EGF、EGFR基因表达特征及与汗腺形成的关系研究. 创伤外科杂志, 2006, 8(1): 65-68.

Zhou G, Fu X B, Chen W, Li H H, Jiang D Y, Bai X D, Lei Y H, Sun T Z. Gene expression characteristics of EGF and its receptor EGFR in fetal skins and appendages. Journal of Traumatic Surgery, 2006, 8(1): 65-68. (in Chinese)

[11]程  飚, 付小兵, 盛志勇, 孙同柱, 孙晓庆. 胎儿、成人正常皮肤EGFR与FGFR-2表达的研究. 中华整形外科杂志, 2003, 19(2): 91-94.

Cheng B, Fu X B, Sheng Z Y, Sun T Z, Sun X Q. A comparative study of EGFR and FGFR-2 expression in fetal and adult skin. Chinese Journal of Plastic Surgery, 2003, 19(2): 91-94. (in Chinese)

[12]陈  伟, 付小兵, 孙同柱, 赵志力, 杨银辉, 盛志勇. 表皮细胞生长因子及其受体在胎儿皮肤组织内的表达特征及其意义. 中国临床康复, 2002, 6(8): 1128-1130.

Chen W, Fu X B, Sun T Z, Zhao Z L, Yang Y H, Sheng Z Y. The expression characteristics and their biological significance of epidermal growth factor and its receptor in fetal and postnatal skins. Modern Rehabilitation, 2002, 6(8): 1128-1130. (in Chinese)

[13]Akiyama M, Smith L T, Holbrook K A. Growth factor and growth factor receptor localization in the hair follicle bulge and associated tissue in human fetus. Journal of Investigative Dermatology, 1996, 106(3): 391-396.

[14]Saga K. Structure and function of human sweat glands studied with histochemistry and cytochemistry. Progress in Histochemistry and Cytochemistry, 2002, 37(4): 323-386.

[15]Li H H, Zhou G, Fu X B, Zhang L. Antigen expression of human eccrine sweat glands. Journal of Cutaneous Pathology, 2009, 36(3): 318-324.

[16]李桂芳, 黄 斌, 杨  武. 表皮生长因子(EGF)对绵羊毛囊体外培养影响的研究. 中国草食动物, 2007, 27(3): 12-14.

Li G F, Huang B, Yang W. Study on the influence by epidermal growth factor(EGF) to the culture of sheep hair follicle. China Herbivores, 2007, 27(3): 12-14. (in Chinese)

[17]史明艳, 林  峰, 杨学义, 窦忠英. 不同浓度的EGF对山羊毛囊干细胞体外培养的影响. 西北农业学报, 2009, 18(3): 26-28.

Shi M Y, Lin F, Yang X Y, Dou Z Y. Effect of different concentrations of EGF on the culture of goat hair follicle stem cells. Acta Agriculturae Boreali-occidentalis Sinica, 2009, 18(3): 26-28. (in Chinese)

[18]崔志峰, 赵  晶, 王  慧, 郝志茹, 张兆远. 不同培养基对山羊毛囊体外培养的影响研究. 山东大学学报: 理学版, 2008, 43(5): 1-5.

Cui Z F, Zhao J, Wang H, Hao Z R, Zhang Z Y. Influences of different mediums on goat hair follicles cultured in vitro. Journal of Shandong University: Natural Science, 2008, 43(5): 1-5. (in Chinese)

[19]姚玉园, 刘海英, 袁  缨, 丛玉艳. IGF-1和EGF对辽宁绒山羊初级毛囊体外培养的影响. 沈阳农业大学学报, 2010, 41(2): 165-169.

Yao Y Y, Liu H Y, Yuan Y, Cong Y Y. Influences of IGF-1 and EGF  on primary hair follicles of Liaoning Cashmere goat in vitro. Journal of Shenyang Agricultural University, 2010, 41(2): 165-169. (in Chinese)

[20]崔景香, 尹逊河, 王  慧. 动物毛囊器官的分化与体外培养的研究进展. 山东农业大学学报: 自然科学版, 2008, 39(3): 489-491.

Cui J X, Yin X H, Wang H. Research advances on the animal hair follicles development and cultivation in vitro. Journal of Shandong Agricultural University: Natural Science, 2008, 39(3): 489-491. (in Chinese)

[21]赵宗胜, 王银林, 李大全, 李桂芳. IGF-1和EGF对绵羊毛囊体外培养的影响. 南京农业大学学报, 2006, 29(2): 138-141.

Zhao Z S, Wang Y L, Li D Q, Li G F. Effects of IGF-1 and EGF on in vitro growth of hair follicles of sheep. Journal of Nanjing Agricultural University, 2006, 29(2): 138-141. (in Chinese)

[22]张燕军, 尹  俊, 李长青, 李金泉. 内蒙古阿尔巴斯绒山羊胎儿期皮肤毛囊发生发育规律研究. 畜牧兽医学报, 2006, 37(8): 761-768.

Zhang Y J, Yin J, Li C Q, Li J Q. Study on development of skin and hair follicle from fetal Inner Mongolian Arbas Cashmere goats. Acta Veterinaria et Zootechnica Sinica, 2006, 37(8): 761-768. (in Chinese)

[23]王树迎, 尹逊河, 孙淑红, 张金花, 马玉忠. 大尾寒羊皮肤组织的生长发育规律. 中国兽医杂志, 2001, 37(10): 8-10.

Wang S Y, Yin X H, Sun S H, Zhang J H, Ma Y Z. Development of the skins of Long-tail Han sheep. Chinese Journal of Veterinary Medicine, 2001, 37(10): 8-10. (in Chinese)

[24]付小兵, 孙同柱, 杨银辉, 顾小曼, 孙晓庆, 盛志勇. 表皮细胞生长因子和碱性成纤维细胞生长因子在不同发育阶段大鼠皮肤表达特征的比较性研究. 中国修复重建外科杂志, 2001, 15(6): 321-324.

Fu X B, Sun T Z, Yang Y H, Gu X M, Sun X Q, Sheng Z Y. The expression characteristics and their biological significance of epidermal growth factor and basic fibroblast growth factor in rat skins at different development stages. Chinese Journal of Reparative and Reconstructive Surgery, 2001, 15(6): 321-324. (in Chinese)

[25]晋  桦, 陈  伟, 孙同柱, 葛世丽, 姜笃银, 周  岗, 付小兵. 胎儿和出生后机体皮肤内表皮生长因子和两种原癌基因表达变化及其与胎儿无瘢痕愈合的关系. 感染、炎症、修复, 2003, 4(2): 121.

Jin H, Chen W, Sun T Z, Ge S L, Jiang D Y, Zhou G, Fu X B. The relationship between the different expression of EGF in fetal and adult and repair with no scar in fetal. Infection, Inflammation, Repair, 2003, 4(2): 121. (in Chinese)

[26]Hansen L A, Alexander N, Hogan M E, Sundberg J P, Dlugosz A, Threadgill D W, Magnuson T, Yuspa S H. Genetically null mice reveal a central role for epidermal growth factor receptor in the differentiation of the hair follicle and normal hair development. American Journal of Pathology, 1997, 150(6): 1959-1975.

[27]杨希川. 毛囊形态发生的基因调控研究进展. 国外医学生理、病理科学与临床分册, 2002, 22(6): 633-636.

Yang X C. Research advances on gene regulation of hair follicles. Foreign Medical Sciences•Section of Pathophysiology and Clinical Medicine, 2002, 22(6): 633-636. (in Chinese)

[28]郭  杏, 谭美云, 熊爱兵, 廖  毅, 何晓川, 郭  力. rhFGF与rhEGF分期使用修复深Ⅱ度烧伤创面的临床观察. 中国药房, 2010, 21(28): 2656-2657.

Guo X, Tan M Y, Xiong A B, Liao Y, He X C, Guo L. Clinical observation on deep Ⅱ degree burn wounds treated by stage with rhFGF and rhEGF at different stages. China Pharmacy, 2010, 21(28): 2656-2657. (in Chinese)

[29]陈  伟, 付小兵, 葛世丽, 孙同柱, 周  岗, 赵志力, 盛志勇.  bFGF, EGF, TGF-β异构体与其受体在胎儿和成人皮肤中的表达特征. 解放军医学杂志, 2003, 28(11): 1001-1004.

Chen W, Fu X B, Ge S L, Sun T Z, Zhou G, Zhao Z L, Sheng Z Y. Expression characteristics of bFGF, EGF, TGF-β isoforms and their receptors in skin form fetus and adult. Medical Journal of Chinese People’s Liberation Army, 2003, 28(11): 1001-1004. (in Chinese)
[1] LI XiaoJing,ZHANG SiYu,LIU Di,YUAN XiaoWei,LI XingSheng,SHI YanXia,XIE XueWen,LI Lei,FAN TengFei,LI BaoJu,CHAI ALi. Establishment and Application of Rapid Quantitative Detection of Viable Plasmodiophora brassicae by PMAxx-qPCR Method [J]. Scientia Agricultura Sinica, 2022, 55(10): 1938-1948.
[2] CHEN Yuan,CAI He,LI Li,WANG LinJie,ZHONG Tao,ZHANG HongPing. Alternative Splicing of TNNT3 and Its Effect on the Differentiation of MuSCs in Goat [J]. Scientia Agricultura Sinica, 2021, 54(20): 4466-4477.
[3] ZHAO XuSheng,QI YongZhi,ZHEN WenChao. Composition and Distribution Characteristics of Pathogens Causing Wheat Sharp Eyespot in Wheat and Maize Double Cropping System [J]. Scientia Agricultura Sinica, 2020, 53(16): 3269-3279.
[4] CHEN HuaFeng,TIAN KeChuan,HUANG XiXia,Ablat Sulayman,HE JunMin,TIAN YueZhen,XU XinMing,FU XueFeng,ZHAO BingRu,ZHU Hua,Hanikezi Tulafu. Construction of Co-expression Network of lncRNA and mRNA Related to Hair Follicle Development of Subo Merino Sheep [J]. Scientia Agricultura Sinica, 2019, 52(19): 3471-3484.
[5] WANG Duo,XIE XueWen,CHAI ALi,SHI YanXia,LI BaoJu. Identification of the Pathogen Causing Cabbage Died in Gansu Province and Detection of Anastomosis Groups [J]. Scientia Agricultura Sinica, 2019, 52(16): 2787-2799.
[6] LIU XiaoChen, WU ShengYong, LEI ZhongRen, WANG HaiHong. Growth Kinetics and Virulence of Two Beauveria bassiana Strains in Frankliniella occidentalis Under Different Temperatures [J]. Scientia Agricultura Sinica, 2018, 51(8): 1484-1492.
[7] JIN ChengYan, Lü XiaoYang, GAO Wen, WANG Yue, CHEN WeiHao, SHENG ShuiXing, CHEN Ling, LIN Jie, SUN Wei. Study on the Relationship Between the Expression of Candidate miRNAs and the Developmental Characteristics in Different Patterns in Hu Sheep Lambskin [J]. Scientia Agricultura Sinica, 2018, 51(14): 2814-2824.
[8] TianBo DING, XiaoBei LIU, Jie LI, KeKe WEI, Dong CHU. Development of a Real-Time Fluorescent Quantitative PCR Method for the Detection of Tomato chlorosis virus and Its Application [J]. Scientia Agricultura Sinica, 2018, 51(10): 2013-2022.
[9] LIU Shan, WANG LiuHao, GUO Jun, LI JiLian, XU LongLong. The Variation of Pathogens, Parasites and Symbionts in Migratory Honeybees (Apis mellifera ligustica) [J]. Scientia Agricultura Sinica, 2017, 50(5): 951-958.
[10] WANG Jun-juan, MU Min, WANG Shuai, LU Xu-ke, CHEN Xiu-gui, WANG De-long, FAN Wei-li, YIN Zu-jun, GUO Li-xue, YE Wu-wei, YU Shu-xun. Molecular Clone and Expression of GhDHN1 Gene in Cotton (Gossypium hirsutum L.) [J]. Scientia Agricultura Sinica, 2016, 49(15): 2867-2878.
[11] WANG Ying-chao, GAN Qin-hua, LI Yan, JI Ying, WU Xing-hai, SHAO Xiu-ling. Detection of Embellisia allii Using Real-Time Quantitative PCR Based on Glyceraldehyde-3-Phosphate Dehydrogenase Gene [J]. Scientia Agricultura Sinica, 2015, 48(2): 390-397.
[12] JIN Xin, ZHANG Man, FAN Yan-ru, WANG Pei, YANG Yin-feng. Effects of Saccharomyces cerevisiae on the Expression of SBD-1 in Cultured Ruminal Epithelial Cells of Sheep [J]. Scientia Agricultura Sinica, 2015, 48(19): 3910-3918.
[13] WANG Yan, LI Da-qi, LIU Xiao-jian, LI Tao, MA En-bo, FAN Ren-jun, ZHANG Jian-zhen. Molecular Characterization and RNAi-Based Functional Analysis of Obstructor Family Genes in Locusta migratoria [J]. Scientia Agricultura Sinica, 2015, 48(1): 73-82.
[14] ZHANG Wen-Hui, HOU Hai-Xia, YANG Qiong, WANG Shu-Hui, MAO Xu-Lian, LIU Yong-Jie. Effects of Tebufenozide on mRNA Expression of Dopa Decarboxylase Gene in Larvae of Spodoptera litura Fabricius [J]. Scientia Agricultura Sinica, 2014, 47(8): 1512-1521.
[15] ZHANG Jian-Qin, GE Ping-Ting, LI Da-Qi, WANG Yan, ZHANG Jian-Zhen, MA EnBo. Spatio-Temporal Expression and Insecticide Tolerance Analysis of Carboxylesterase Gene LmCesF1 from Locusta migratoria [J]. Scientia Agricultura Sinica, 2014, 47(8): 1522-1530.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备09089781号-30
Copyright 2018 © Editorial office of Scientia Agricultura Sinica
Tel: 86-010-82106279    E-mail: zgnykx@mail.caas.net.cn
Supported by Beijing Magtech Co.Ltd