Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (1): 184-191.doi: 10.3864/j.issn.0578-1752.2012.01.022

• VETERINARY SCIENCE • Previous Articles     Next Articles

Expression of TRPV6 in Different Reproductive Organs in Laying Hens

 YANG  Jun-Hua, HOU  Jia-Fa, DENG  Yi-Feng, ZHOU  Zhen-Lei, WANG  Yong-Mei, SHI  Lu-Juan   

  1. 1.南京农业大学动物医学院，南京210095
    2.上海市农业科学院农产品质量标准与检测技术研究所，上海 201403
    3.新乡市畜产品质量检测检验中心，河南新乡 453003
    4.常熟市畜牧兽医站，江苏常熟 215500
  • Received:2011-02-10 Online:2012-01-01 Published:2011-11-18

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Abstract: 【Objective】 The aim of this study was to investigate the localization and expression of transient receptor potential vanilloid receptor 6 (TRPV6) in the ovary, magnum, isthmus and uterus parts of the oviduct in laying hens. 【Method】Immunohistochemical technology and RT-PCR were used to detect the localization of TRPV6 in different reproductive organs. In addition, quantitation of TRPV6 mRNA level and protein concentration in these tissues were carried out by real-time PCR and Western blotting analysis, respectively. 【Result】 Positive expression of the TRPV6 protein and mRNA was observed in ovarium, magnum, isthmus and uterus parts of the oviduct in laying hens, respectively. TRPV6 was localized in the primary follicle and secondary follicle in the ovary. Furthermore, intense staining was observed in villus epithelial cell (face to lumen) of mucous epithelium of the magnum, isthmus and uterus parts of the oviduct. And the weaker staining was also found in the basilar layer of endometrium in the uterus parts of the oviduct. Moreover, the mRNA levels of TRPV6 in the magnum and isthmus were significantly decreased compared with that in the ovary (P＜0.05). The expression of TRPV6 in the magnum was also lower than that in the ovary (P＜0.05). However, the expression of TRPV6 mRNA in the uterus parts of the oviduct was lower than that in the ovary, but the change of protein concentration was in contrast, and the difference was not significant (P＞0.05).【Conclusion】 The expression of TRPV6 was detected in ovarium, magnum, isthmus and uterus parts of the oviduct in laying hens. And the higher concentration of TRPV6 in ovary and uterus parts implied that the TRPV6 may be not only involved in the development and maturation, but also may play an important role in Ca2+ transport in ESG (eggshell gland) during eggshell calcification.

Key words: TRPV6, ovary, magnum, isthmus, uterus, oviduct, laying hens

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