Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (23): 4926-4932.doi: 10.3864/j.issn.0578-1752.2011.23.020

• VETERINARY SCIENCE • Previous Articles     Next Articles

Antioxidative Function of Lacbacillus rhamnosus to Caco-2 Cells

 CUI  Zhi-Wen, HUANG  Qin, HUANG  Yi, WU  Hong-Zhao, WEN  Jing, LI  Wei-Fen   

  1. 1.浙江大学动物科学学院/教育部动物分子营养学重点实验室，杭州 310029
    2.广西大学动物科学技术学院，南宁 530005
  • Received:2010-11-17 Online:2011-12-01 Published:2011-05-23

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Abstract: 【Objective】This experiment was conducted to study the antioxidative function of Lactobacillus rhamnosus to Caco-2 cells under oxidative stress. 【Method】 Caco-2 cells were randomly divided into 4 groups, control group and oxidative stress group (100 µmol•L-1 H2O2), treatment groupsⅠandⅡ were added with 1 mL Lactobacillus rhamnosus (about 108 CFU•mL-1) and tertiary butylhydroquinone (TBHQ) (2.75 µg•mL-1 ) under oxidative stress, respectively. Antioxidative activities in culture supernatants and lysates of Caco-2 cells at 12 h and 48 h were measured. 【Result】 Compared to oxidative stress group, the total antioxidation capacity (T-AOC), the glutathione peroxidase (GSH-Px) (P＜0.01), catalase (CAT) (P＜0.01) activities in the cultured supernatants, and the superoxide dismutase (SOD) activity (P＜0.01), and the glutathione (GSH) content (P＜0.05) in the cells lysates at 12 h in treatment groupⅠincreased, and the increases of the antisuperoxide anion radical (O2-) (P＜0.01), GSH-Px (P＜0.01), CAT (P＜0.01), SOD (P＜0.01) activities in the cells culture supernatants and the peroxidase (POD) activity (P＜0.01) and the decreases of malondialdehyde (MDA) content (P＜0.01) in the cells lysates at 48 h were also observed. In treatment groupⅡ, the T-AOC (P＜0.01), CAT activity (P＜0.01) in the cells culture supernatants and the glutathione (GSH) content (P＜0.01) in the cells lysates at 12 h increased significantly, but these values and the MDA content at 48 h decreased significantly (P＜0.01). The anti-superoxide anion radical (O2-), SOD, GSH-Px, POD activities in the cells culture supernatants and the POD activity in the cells lysates at 48 h increased (P＜0.05).【Conclusion】These results indicated that adding Lactobacillus rhamnosus could increase the antioxidative function of Caco-2 cells under oxidative stress under in vitro conditions.

Key words: Caco-2 cells, Lactobacillus rhamnosus, TBHQ, antioxidative activities, oxidative stress

[1] Lykkesfeldt J, Svendsena O. Oxidants and antioxidants in disease: oxidative stress in farm animals. The Veterinary Journal, 2007, 173(3): 502-511.

[2] Masona L M, Hogana S A, Lyncha A, Sullivanb K O, Lawlorc P G, Kerrya J P. Effects of restricted feeding and antioxidant supplementation on pig performance and quality characteristics of longissimus dorsi muscle from Landrace and Duroc pigs. Meat Science, 2005, 70(2): 307-317.

[3] Gorbach S L. Probiotics and gastrointestinal health. American Journal of Gastroenterology, 2000, 95: S2-S4.

[4] 马鹏飞, 陈有亮, 金鸟君. 鼠李糖乳杆菌功能特性的研究进展. 科技通报, 2009, 25(2): 202-206.

Ma P F, Chen Y L, Jin N J. Research and development on the functional properties of Lactobacillas rhamnosus. Bulletin of Science and Technology, 2009, 25(2): 202-206. (in Chinese)

[5] Peuhkuri K, Lahteenmaki T, Sievi E, Saxelin M, Vapaatalo H, Korpela R. Antioxidative properties of Lactobacillus GG measured as prostacyclin and nitric oxide production in endothelial cell culture. Nutrion of Today(Suppl.), 1996, 31(6): S39-S40.

[6] Korpela R, Peuhkuri K, Laehteenmaeki T, Sievi E, Saxelin M, Vapaatalo H. Lactobacillus rhamnosus GG shows antioxidative properties in vascular endothelial cell culture. Milchwissenschaft-Milk Science International, 1997, 52(9): 503-505.

[7] 肖丽霞, 王慧晶, 顾瑞霞, 杨振泉, 张宜凤, 朱小红, 孙  云. 乳酸菌对小鼠急性酒精性肝损伤的保护作用. 扬州大学学报: 农业与生命科学版, 2008, 29(l): 37-41.

Xiao L X, Wang H J, Gu R X, Yang Z Q, Zhang Y F, Zhu X H, Sun Y. Study On the protective effects of lactic acid bacteria on acute aIcohol induced Iiver injury in mice. Journal of Yangzhou University: Agricultural and Life Science Edition, 2008, 29(l): 37-41. (in Chinese)

[8] Kaizua H, Sasakia M, Nakajimaa H, Suzukia Y. Effect of antioxidative lactic acid bacteria on rats fed a diet deficient in vitamin E. Journal of Dairy Science, 1993, 76(9): 2493-2499.

[9] Ahotupa M, Saxelin M, Korpela R. Antioxidative properties of Lactobacillus GG. Nutrion of Today(Suppl.), 1996, 31(6): S51-S52.

[10] CIR. 3 Final report on the safety assessment of t-butyl hydroquinone. International Journal of Toxicology, 1986, 5(5): 329-351.

[11] Domingos A K, Saad E B, Vechiatto W W D, Wilhelm H M, Ramos L P. The influence of BHA, BHT and TBHQ on the oxidation stability of soybean oil ethyl esters (Biodiesel). Journal of the Brazilian Chemical Society, 2007, 18(2): 416-423.

[12] Shen G, Jeong W S, Hu R, Kong A N. Regulation of Nrf2, NF-κB, and AP-1 signaling pathways by chemopreventive agents. Antioxidants and Redox Signaling, 2005, 7(11-12): 1648-1663.

[13] Eftekharzadeh B, Maghsoudi N, Khodagholi F. Stabilization of transcription factor Nrf2 by tBHQ prevents oxidative stress-induced amyloid β formation in NT2N neurons. Biochimie, 2010, 92(3): 245-253.

[14] Abdel-Wahab M H. Testicular toxicity of dibromoacetonitrile and possible protection by tertiary butylhydroquinone. Pharmacological Research, 2003, 47(6): 509-515.

[15] 张江巍, 曹郁生, 李海星, 陈  燕. 乳酸菌抗氧化活性及检查方法. 中国乳品工业, 2005, 33(9): 53-55.

Zhang J W, Cao Y S, Li H X, Chen Y. Antioxidative activities of lactic acid bacteria and the test method. China Dairy Industry, 2005, 33(9): 53-55. (in Chinese)

[16] 张书文, 吕加平, 孟和毕力格, 刘  鹭, 胡鲜宝. 干酪乳杆菌干酪亚种Lactobacillus casei subsp.casei SY13对衰老模型小鼠的抗氧化作用. 中国农业科学, 2010, 43(10): 2141-2146.

Zhang S W, Lü J P, Menghe B L G, Liu L, Hu X B. Antioxidative activity of Lactobacillus casei subsp.casei SY13 on ageing model mice. Scientia Agricultura Sinica, 2010, 43(10): 2141-2146. (in Chinese)

[17] 商丰才, 黄  琴, 李卫芬. 6种新型抗氧化剂及组合清除自由基活性的研究. 饲料研究, 2008, 7: 1-4.

Shang F C, Huang Q, Li W F. Study on free radical active scavenging by 6 kinds of new type antioxidant. Feed Research, 2008, 7: 1-4. (in Chinese)

[18] Ko Y H, Yang H Y, Jang I S. Effect of conjugated linoleic acid on intestinal and hepatic antioxidant enzyme activity and lipid peroxidation in broiler. Asian-Australasian Journal of Animal Science, 2004, 17: 1162-1167.

[19] Hewitt J, Morris J G. Superoxide dismutase in some obligately anaerobic bacteria. Febs Letter, 1975, 50: 315-318.

[20] Frank V, Eva V, James F D. The role of active oxygen species in plant signal transduction. Plant Science, 2001, 161: 405-414.

[21] Draper H H, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Methods in Enzymology, 1990, 186: 421-431.

[22] Bilici M, Efe H, Köro?lu M A, Uydu H A, Bekaro?lu M, De?er O. Antioxidative enzyme activities and lipid peroxidation in major depression: alterations by antidepressant treatments. Journal of Affective Disorders, 2001, 64(1): 43-51.

[23] Kullisaar T, Zilmer M, Mikelsaar M, Vihalemm T, Annuk H, Kairane C, Kilk A. Two antioxidative lactobacilli strains as promising probiotics. International Journal of Food Microbiology, 2002, 72(3): 215-224.

[24] Kullisaar T, Songisepp E, Mikelsaar M, Zilmer K, Vihalemm T, Zilmer M. Antioxidative probiotic fermented goats’milk decreases oxidative stress-mediated atherogenicity in human subjects. British Journal of Nutrition, 2003, 90(2) : 449-456.

[25] Koller V J, Marian B, Stidl R, Nersesyan A, Winter H, Simi? T, Sontag G, Knasmüller S. Impact of lactic acid bacteria on oxidative DNA damage in human derived colon cells. Food and Chemical Toxicology, 2008, 46: 1221-1229.

[26] 王玉华, 王立梅, 冯  印, 高  晶, 胡耀辉. 鼠李糖乳杆菌菌    株LR12 和LR76 的抗氧化性研究. 食品科学, 2009, 30(19): 177-180.

Wang Y H, Wang L M, Feng Y, Gao J, Hu Y H. Antioxidant activity of Lacbacillus rhamnosus LR12 and LR76. Food Science, 2009, 30(19): 177-180. (in Chinese)

[27] 杨静秋. 抗氧化乳酸菌的筛选及其对氧化损伤的CT-26细胞的保护作用[D]. 无锡: 江南大学, 2009: 5-10.

Yang J Q. Screening of anti-oxidative lactic acid bacteria and its protecting effect to oxidative damaged CT-26 cells[D]. Wuxi: Jiangnan University, 2009: 5-10. (in Chinese)

[28] Tao Y, Drabik K A, Waypa T S, Musch M W, Alverdy J C, Schneewind O, Chang E B, Petrof E O. Soluble factors from Lactobacillus GG activate MAPKs and induce cytoprotective heat shock proteins in intestinal epithelial cells. American Journal of Physiology-Cell Physiology, 2006, 290: C1018-C1030.

[29] Ryu K. The characteristics of performance and exhaust emissions of a diesel engine using a biodiesel with antioxidants. Bioresource Technology, 2010, 101(1): S78-S82.

[30] Li J, Johnson D, Calkins M, Wright L, Svendsen C, Johnson J. Stabilization of Nrf2 by TBHQ confers protection against oxidative stress-induced cell death in human neural stem cells. Toxicological Sciences, 2005, 83(2): 313-328.

[31] 李  航, 任韫卓, 刘淑霞, 刘青娟, 刘品力, 周  琰, 段惠军. 叔丁基对苯二酚对糖尿病小鼠肾脏氧化应激损伤及Nrf2蛋白表达的影响. 中国药理学通报, 2009, 25(10): 1341-1345.

Li H, Ren Y Z, Liu S X, Liu Q J, Liu P L, Zhou Y, Duan H J. Effects of TBHQ on the oxidative stress and the expression of Nrf2 in kidneys of diabetic mice. Chinese Pharmacological Bulletin, 2009, 25(10): 1341-1345. (in Chinese)

[32] 武小媛, 曲丽艳, 全  康, 蒋燕灵, 唐修文. tBHQ和Sulforaphane对Caco-2细胞Nrf2-ARE信号通路的影响. 浙江大学学报: 医学版, 2010, 39(1): 17-23.

Wu X Y, Qu L Y, Quan K, Jiang Y L, Tang X W. Effect of tBHQ and sulforaphaneon Nrfa-ARE signaling pathway of Caco-2 cells. Journal of Zhejiang University: Medical Sciences, 2010, 39(1): 17-23. (in Chinese)
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