Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (16): 3413-3419.doi: 10.3864/j.issn.0578-1752.2011.16.014

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Development of Equine-Bovine Interspecies Cloned Embryos

ZHU  Hua-Bin, SHEN  Yan-Jun, DU  Wei-Hua, JU  Guang-Wei, PANG  Yun-Wei, HAO  Hai-Sheng, ZHAO  Xue-Ming, WANG  Dong, WANG  Zong-Li   

  1. 1. 甘肃农业大学动物科学技术学院
    2. 中国农业科学院北京畜牧兽医研究所
    3. 山东大学齐鲁医院
  • Received:2009-12-01 Revised:2010-05-07 Online:2011-08-15 Published:2011-05-05

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Abstract: 【Objective】The objective of the study was to investigate interspecies somatic cell nuclear transfer (iSCNT) embryonic potential during preimplantation development.【Method】The equine- bovine embryos were reconstructed via hand-made cloning using bovine oocytes as recipient cytoplasm and equine fibroblast as donor cells. Karyotype and the developmental rate were analyzed. 【Result】 Activated with A23187 + 6-DMAP, the iSCNT embryos could develop to blastocyst (2.60%). The cleavage rate and morual rate of cloned embryos cultured in mCR1aa were higher than CR1aa and SOF. Blastocysts were harvested in mCR1aa (2.72%). But there was no difference between CR1aa and SOF. No significant difference was observed in the developmental capacity of cloned embryos and the quality of donor treated with serum starvation or confluence inhibition. The fusion rate of embryos cloned by HMC was higher than microinjection method (90.33% vs 72.94%). Compared equine-bovine iSCNT with bovine–bovine SCNT, there was a significant difference in morual rate and blastocyst rate (21.78% vs 63.52%；2.71% vs 37.48%). 【Conclusion】 It is concluded that the bovine oocytes can support the dedifferentiation and reprogramming of horse somatic cell. However, due to the distance between the two species, the capacity of development of interspecies cloned embryos is far lower than the same species cloned embryos.

Key words: Equine, Bovine, Interspecies clone, Hand-made clone

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