转Bt基因抗虫玉米根茬和根际土壤中Cry1Ab杀虫蛋白的田间降解动态

doi:10.3864/j.issn.0578-1752.2010.23.024

Abstract

Abstract:

【Objective】 The field degradation dynamics of Cry1Ab insecticidal protein in transgenic Bacillus thuringiensis corn in the root debris and rhizosphere soil of corn of events MON810 and Bt11 at harvest stage were investigated and compared. 【Method】 The levels of Cry1Ab protein expression in root debris and rhizosphere soil of the two transgenic Bt corns of events MON810 and Bt11were quantified by using enzyme-linked immunosorbent essay (ELISA). 【Result】 It was showed that the Cry1Ab toxin in Bt corn root debris and rhizosphere soil were gradually degraded. The content of Cry1Ab insecticidal protein in corn root debris of MON810 was higher than that of Bt11, the degradation speed was slower compared with that of Bt11. The Cry1Ab insecticidal protein in root debris could not degrade completely in 8 months after harvest. No Cry1Ab insecticidal protein was detected in root debris of Bt11 in 7 months after harvest. Degradation of Cry1Ab insecticidal protein in rhizosphere soil of event MON810 was slower than that of event Bt11, no Cry1Ab insecticidal protein was detected in the rhizosphere soil of events MON810 and Bt11 in 8 months and 7 months after harvest, respectively. 【Conclusion】 The Cry1Ab insecticidal protein could not degrade completely, and some residues still remain in root debris and rhizosphere soil of events MON810 and Bt11 in the emerging stage of the spring crops in following year in field where the MON810 and Bt11 were planted.

Key words: Bt transgenic corn, root debris, rhizosphere soil, Cry1Ab protein, degradation

XING Zhen-juan,WANG Zhen-ying,HE Kang-lai,BAI Shu-xiong
.

Degradation Dynamics of Cry1Ab Insecticidal Protein Within Transgenic Bacillus thuringiensis Corn Root Debris and Rhizosphere Soil in Field

[J].Scientia Agricultura Sinica, 2010, 43(23): 4970-4976 .

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2010.23.024

https://www.chinaagrisci.com/EN/Y2010/V43/I23/4970

References

Related Articles 15

[1]	FENG Chen,FENG LiangShan,LIU Qi,LI HaoRu,ZHENG JiaMing,YANG Ning,BAI Wei,ZHANG Zhe,SUN ZhanXiang. The Degradation Characteristics of Different Plastic Films and Their Effects on Maize Yield in Semi-Arid Area in Western Liaoning [J]. Scientia Agricultura Sinica, 2021, 54(9): 1869-1880.
[2]	ZHAO WeiSong,GUO QingGang,SU ZhenHe,WANG PeiPei,DONG LiHong,HU Qing,LU XiuYun,ZHANG XiaoYun,LI SheZeng,MA Ping. Characterization of Fungal Community Structure in the Rhizosphere Soil of Healthy and Diseased-Verticillium Wilt Potato Plants and Carbon Source Utilization [J]. Scientia Agricultura Sinica, 2021, 54(2): 296-309.
[3]	YAN RuiRui, GAO Wa, SHEN BeiBei, ZHANG Yu, WANG Miao, ZHU XiaoYu, XIN XiaoPing. Index System for Quantitative Evaluation of Pasture Degradation in Meadow Grassland of Inner Mongolia [J]. Scientia Agricultura Sinica, 2021, 54(15): 3343-3354.
[4]	Na JIANG,DongMei SHI,GuangYi JIANG,Ge SONG,ChengJing SI,Qing YE. Effects of Soil Erosion on Physical and Mechanical Properties of Cultivated Layer of Purple Soil Slope Farmland [J]. Scientia Agricultura Sinica, 2020, 53(9): 1845-1859.
[5]	WANG DeLi,WANG Ling,XIN XiaoPing,LI LingHao,TANG HuaJun. Systematic Restoration for Degraded Grasslands: Concept, Mechanisms and Approaches [J]. Scientia Agricultura Sinica, 2020, 53(13): 2532-2540.
[6]	QIU CunPu, CHEN XiaoFen, LIU Ming, LI WeiTao, WU Meng, JIANG ChunYu, FENG YouZhi, LI ZhongPei. Microbial Transformation Process of Straw-Derived C in Two Typical Paddy Soils [J]. Scientia Agricultura Sinica, 2019, 52(13): 2268-2279.
[7]	LIU YuHuai, WEI XiaoMeng, WEI Liang, ZHU ZhenKe, GE TiDa, ZHANG YanJie, LU ShunBao, WU JinShui. Responses of Extracellular Enzymes to Carbon and Phosphorus Additions in Rice Rhizosphere and Bulk Soil [J]. Scientia Agricultura Sinica, 2018, 51(9): 1653-1663.
[8]	GE Bo, WANG BaoBao, GUO Cheng, SUN SuLi, CHEN GuoKang, WANG XiaoMing, ZHU ZhenDong, DUAN CanXing. Composition and quantitative analysis of Fusarium species in maize rhizosphere soil [J]. Scientia Agricultura Sinica, 2018, 51(19): 3683-3693.
[9]	AN TingTing, HOU XiaoPan, ZHOU YaNan, LIU WeiLing, WANG Qun, LI ChaoHai, ZHANG XueLin. Effects of Nitrogen Fertilizer Rates on Rhizosphere Soil Characteristics and Yield After Anthesis of Wheat [J]. Scientia Agricultura Sinica, 2017, 50(17): 3352-3364.
[10]	JI Cheng, JIA Ru, ZHAO LiHong. Application of Gene Engineering Technique in Aflatoxin Biodegradation [J]. Scientia Agricultura Sinica, 2017, 50(17): 3422-3428.
[11]	CHEN Li-juan, LI Xin, LI Zheng, LI Pei-di, LI Zhong-wen, ZHANG De-quan. Protein Phosphorylation on the Function of Myofibrillar Proteins in Mutton Muscle [J]. Scientia Agricultura Sinica, 2016, 49(7): 1360-1370.
[12]	SONG Hui-fang, LI Ying-long, MA En-bo, ZHANG Jian-zhen. The Heterogenous Expression and Enzymatic Characteristics of β-N-acetylglucosaminidase from Locusta migratoria [J]. Scientia Agricultura Sinica, 2016, 49(21): 4140-4148.
[13]	ZHANG Xue-lin, XU Jun, AN Ting-ting, HOU Xiao-pan, LI Chao-hai. Relationship Between Rhizosphere Soil Properties and Yield of Maize at Different Nitrogen Levels [J]. Scientia Agricultura Sinica, 2016, 49(14): 2687-2699.
[14]	ZHONG Ping, CHEN Pu-rui, WANG Qian, XIAO Fu-liang, ZHANG Kuan, MA Fu-rong, HUANG Mei-ling, WANG Ping-rong, DENG Xiao-jian, SUN Chang-hui. Genetic Analysis and Candidate Gene Identification of the Panicle Degradation Mutant spd11 in Rice [J]. Scientia Agricultura Sinica, 2016, 49(10): 1835-1843.
[15]	CHEN Zeng-long, CHEN Xiu, DONG Feng-shou, LIU Xin-gang, XU Jun, ZHENG Yong-quan. Chiral Stability of Dinotefuran in Organic Solvents and Water [J]. Scientia Agricultura Sinica, 2015, 48(9): 1756-1763.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!