脱氧雪腐镰刀菌烯醇降解菌的分离和鉴定

doi:10.3864/j.issn.0578-1752.2010.22.010

Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (22): 4635-4641 .doi: 10.3864/j.issn.0578-1752.2010.22.010

• PLANT PROTECTION • Previous Articles Next Articles

Isolation and Identification of Deoxynivalenol Degradation Strains

XU Jian-hong, JI Fang, WANG Hong-jie, WANG Jian-wei,LIN Fan-yun, SHI Jian-rong

(江苏省农业科学院/农业部食品质量安全监控重点开放实验室/江苏省食品质量安全重点实验室)

Received:2010-04-06 Revised:2010-05-10 Online:2010-11-15 Published:2010-11-15
Contact: SHI Jian-rong

Abstract

Abstract:

【Objective】 The aim of this study was mainly to find bacteria to degrade deoxynivalenol (DON) and realize the application of biodetoxification of DON．【Method】 Wheat was inoculated with a high-yielding strain F. graminearum F-25 for the production of DON, and using DON as the only carbon source for enrichment and isolation of the mixed culture, then screening for the strains which can degrade DON. 【Result】The strain DDS-1 was screened out from 1 000 strains, the degradation ratio of DON reached more than 95%, which was obviously higher than others．According to its morphological, physiological, and biochemical characteristics and 16S rDNA gene sequence,the strain DDS-1 was finally identified as Devosia sp.. After DDS-1 was added to the feedstuff, the toxin in the feed can be degraded by 75.47%. 【Conclusion】The strain DDS-1 that can degrade DON was identified preliminarily as Devosia sp. DDS-1 can not only degrade DON in liquid medium, but also degrade DON in feedstuff. The strain of Devosia sp. can degrade DON, thus supplying a feasible solution for biodetoxification of DON.

Key words: deoxynivalenol, degradation, Devosia sp., biodetoxification

XU Jian-hong,JI Fang,WANG Hong-jie,WANG Jian-wei,LIN Fan-yun,SHI Jian-rong
. Isolation and Identification of Deoxynivalenol Degradation Strains
[J].Scientia Agricultura Sinica, 2010, 43(22): 4635-4641 .

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