Scientia Agricultura Sinica ›› 2010, Vol. 43 ›› Issue (4): 842-849 .doi: 10.3864/j.issn.0578-1752.2010.04.023

• VETERINARY SCIENCE • Previous Articles     Next Articles

Localization of Cytokines in Abomasum of Goats Immunized with Recombinant Galectins of Haemonchus Contortus

ZHANG Li-wu, SUN Yan-ming, XU Li-xin, YAN Ruo-feng, LI Xiang-rui
   

  1. （南京农业大学动物医学院）
  • Received:2007-09-10 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20
  • Contact: LI Xiang-rui

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Abstract:

【Objective】 Cytokines in abomasum of goats immunized with rHco-GAL-f/m were detected in this study. 【Method】 In situ hybridization was used to localize IL-4, IL-6, IL-1?, IL-2,IFN-γ and TNF-α mRNAs in abomasum of the negative control group (A), challenged control group (B), vaccinated-challenged group (C) and vaccinated-unchallenged group (D) goats immunized with rHco-GAL-f/m, and the numbers of cytokine mRNA+ cells in each group were also analyzed. 【Result】 No cytokines were found in the abomasum of group A goats and IL-4, IL-6, IL-1?, IL-2, IFN-γ and TNF-α were detected in the submucosa layer of abomasum of groups B, C and D goats. The difference of IL-4 and IL-6 mRNA+ cell numbers in groups B, C and D was significant (P＜0.05) and group C was higher than that of groups B and D. The difference of IL-1?, IL-2 and, TNF-α mRNA+ cell numbers was also significant (P＜0.05) among groups B,C and D, and group D was the highest. IFN-γ mRNA+ cells were not significantly different between groups B and C (P＞0.05), but IFN-γ mRNA+ cells in group D was significantly different and was higher compared to groups B and C (P＜0.05). 【Conclusion】 The findings indicated that the recombinant galectins of H. contortus are capable of inducing local immune responses in abomasum of goats.

Key words: Haemonchus contoruts, galectin, cytokines, goat

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