关键词: 高效液相色谱, DNA 甲基化, 猪

Abstract: Abstract:【Objective】In this experiment, parental lines Large White、Landrace、Meishan and their hybrids Large White × Landrace、Landrace × Large White、Large White×Meishan、Meishan×Large White were used as the source of DNA samples for the determination of DNA methylation content both in the blood and muscle tissue of pigs, at the same time, the significant difference about DNA methylation content between parental lines and their hybrids, base on different hybrid systems and different tissues, were analyzed.【Method】High performance liquild chromatography, HPLC.【Result】The average DNA methylation content in the 163 samples of muscle tissue is 16.92%, while the average DNA methylation content in the 182 samples of blood is 6.49%, the difference between which is especially prominent (p<0.01). In the same tissue but different hybrid system, hybrids appear different. Similarly, in the same hybrid system but different tissue, hybrids are different too. 【Conclusion】The increase of DNA methylation percentage in hybrids may result in more silence expression of genes which are disbenificial to development. The hybrids methylation percentages differ in hybrid combination and tissue specificity.

Key words: HPLC, DNA methylation, pig