中国农业科学 ›› 2022, Vol. 55 ›› Issue (14): 2696-2708.doi: 10.3864/j.issn.0578-1752.2022.14.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水杨酸引发对红麻幼苗耐盐性的影响

胡亚丽(),聂靖芝,吴霞,潘姣,曹珊,岳娇,罗登杰,王财金,李增强,张辉,吴启境,陈鹏()   

  1. 广西大学农学院/广西高校植物遗传育种重点实验室,南宁 530004
  • 收稿日期:2022-02-27 接受日期:2022-04-24 出版日期:2022-07-16 发布日期:2022-07-26
  • 通讯作者: 陈鹏
  • 作者简介:胡亚丽,E-mail: 2541764968@qq.com
  • 基金资助:
    国家自然科学基金(31960368);国家现代农业产业技术体系建设专项(CARS-16-E14)

Effect of Salicylic Acid Priming on Salt Tolerance of Kenaf Seedlings

HU YaLi(),NIE JingZhi,WU Xia,PAN Jiao,CAO Shan,YUE Jiao,LUO DengJie,WANG CaiJin,LI ZengQiang,ZHANG Hui,WU QiJing,CHEN Peng()   

  1. College of Agriculture, Guangxi University/Guangxi Colleges and Universities Key Laboratory of Plant Genetics and Breeding, Nanning 530004
  • Received:2022-02-27 Accepted:2022-04-24 Online:2022-07-16 Published:2022-07-26
  • Contact: Peng CHEN

摘要:

【目的】研究水杨酸(SA)引发对盐胁迫下红麻生长及生理响应,并揭示SA引发对红麻中逆境相关基因的诱导模式,为红麻耐盐性研究提供理论依据。【方法】以2个不同耐盐性红麻品种(盐抗性材料为CP018,盐感性材料为CP047)为研究对象,将种子引发处理后进行水培试验,分析SA引发对红麻种子萌发及150 mmol·L-1 NaCl胁迫下幼苗农艺性状及生理方面的影响,并通过qRT-PCR技术分析SA引发逆境相关基因的表达模式。【结果】盐抗性品种CP018经过0.2 mmol·L-1 SA引发后,能显著提升种子发芽率、发芽势和发芽指数,分别提高34.78%、31.30%和58.07%;盐感性材料CP047也有一定的提高,分别提高7.50%、10.56%和6.23%,但是未达到显著水平。在盐胁迫条件下,经SA引发(S1)与未引发(N1)相比,株高抑制率在盐抗性和盐感性品种中分别显著降低4.07%(CP018)和3.91%(CP047),干重抑制率在2个品种中分别显著降低15.50%(CP018)和15.68%(CP047);鲜重抑制率在盐感性品种CP047中显著降低4.46%,但在盐抗性品种CP018中未达到显著水平。根系扫描分析表明,根长抑制率在盐抗性和盐感性材料中分别显著下降10.74%(CP018)和10.77%(CP047);根表面积抑制率在盐抗性和盐感性品种中分别下降5.09%(CP018)和2.95%(CP047),仅在盐抗性品种CP018中达到显著水平;而根系活力抑制率在盐感性品种CP047中降低46.21%,在盐抗性品种CP018中降低6.56%,仅在盐感性品种CP047中达到显著水平。灰色关联度分析发现根系活力是对影响植株干重最重要的因素。SA引发能降低盐胁迫下红麻叶片的MDA含量,提高POD和SOD酶活性。对12个逆境相关基因的表达量分析结果表明,ACCDAPX2SOS1ARR2PALERF.C3CHITTIFY11表达水平在SA引发处理下均显著上调,而ERF9ERS1MYC2XTH22在2个材料中的表达模式存在差异,其中,XTH22在CP047中显著上调,在盐抗性品种CP018中无显著变化,ERS1MYC2在盐抗性品种CP018中显著上调,在盐感性品种CP047中却显著下降,而ERF9在2个品种中的趋势则与此相反。【结论】适宜浓度的SA引发可以显著缓解红麻在盐胁迫下的生长,且对不同红麻种质资源的影响程度和方式存在差异,SA可能通过影响生理过程如抗氧化酶系统,并通过诱导特异基因的表达调节红麻植株对非生物胁迫的响应。

关键词: 红麻(Hibiscus cannabinus L.), 种子引发, 水杨酸, 抗氧化酶活, 盐胁迫

Abstract:

【Objective】To study the growth and physiological response of salicylic acid (SA) priming in kenaf under salt stress, and further reveal the induction pattern of SA priming on the stress-related genes in kenaf, thus provide a theoretical basis for salt tolerance study in kenaf. 【Method】Two different salt-tolerant kenaf cultivars (resistant and sensitive cultivars codenamed CP018 and CP047, respectively) were used as materials. The seeds were tested by SA priming and then subjected to hydroponics experiments to analyze the effect of SA priming on kenaf seed germination and the agronomic and physiological aspects of seedling under 150 mmol·L-1 NaCl stress, and the expression patterns of SA priming stress-related genes were analyzed by qRT-PCR. 【Result】The germination rate, germination potential and germination index of the salt-resistant cultivar CP018 were significantly improved after 0.2 mmol·L-1 SA priming, by 34.78%, 31.30% and 58.07%, respectively; the salt-susceptible cultivar CP047 also showed some improvement, by 7.50%, 10.56% and 6.23%, respectively, but did not reached the significant level. Under salt stress conditions, plant height inhibition was significantly reduced by 4.07% (CP018) and 3.91% (CP047) in the 2 cultivars by SA priming (S1) compared with un-priming (N1), and dry weight inhibition was significantly reduced by 15.50% (CP018) and 15.68% (CP047), in the 2 cultivars, respectively; fresh weight inhibition was significantly reduced by 4.46% in CP047, but not in CP018. Analysis of the root systems showed that root length inhibition was significantly reduced by 10.74% (CP018) and 10.77% (CP047) in the two cultivars, respectively, root surface area inhibition decreased by 5.09% (CP018) and 2.95% (CP047) in the two cultivars, reaching a significant level only in the salt-resistant cultivar CP018, while root activity inhibition was significantly reduced by 46.21% in the salt-susceptible cultivar CP047 and 6.56% in the salt-resistant cultivar CP018, reaching a significant level only in the salt-susceptible cultivar CP047. A grey correlation analysis of the indicators revealed that root activity was the most relevant factor influencing plants dry weight. SA priming reduced the MDA content and increased the POD and SOD enzyme activities of kenaf leaves under salt stress. Expression analysis of 12 stress-related genes showed that ACCD, APX2, SOS1, ARR2, PAL, CHIT and TIFY11 genes expression levels were significantly up-regulated after SA priming, while ERF9, ERS1, ERF.C3 and MYC2 and XTH22 expression patterns differed between the two cultivars, with XTH22 being significantly up-regulated in salt sensitive cultivar CP047 but not in the salt resistant cultivar CP018, ERS1 and MYC2 were significantly up-regulated in the salt resistant cultivar CP018 but significantly down regulated in the salt sensitive cultivar CP047, while the trend of ERF9 was opposite in the two cultivars. 【Conclusion】SA priming at a suitable concentration could significantly alleviate the growth of kenaf under salt stress, and differed in the degree and patterns of effects on different kenaf germplasm resources. SA may regulate kenaf plant response to abiotic stresses by affecting physiological processes such as antioxidant enzyme systems and mediating the expression of specific genes.

Key words: kenaf (Hibiscus cannabinus L.), seed priming, salicylic acid, antioxidant enzyme activity, salt stress