赤霉素GA4是水稻矮化特征的重要调节因子

doi:10.3864/j.issn.0578-1752.2019.05.002

摘要/Abstract

摘要：

【目的】以水稻幼胚组培过程中获得的一株半矮化水稻突变体为研究对象,解析水稻半矮化突变体株高变矮及分蘖增多等表型异常的原因,为克服水稻过度矮化发育障碍因子及培育抗倒伏高产水稻品种提供科学理论依据。【方法】首先统计分析半矮化水稻突变体与野生型的表型差异,利用体式显微镜和光学显微镜观察突变体花的结构及其细胞特征;通过转录组测序及qRT-PCR分析差异基因的表达特征,并通过外源喷施赤霉素GA3处理检测突变体对外源赤霉素的敏感性;最后利用高效液相色谱和质谱联仪检测突变体内赤霉素的含量与富集特征。【结果】表型观测与统计结果表明,突变体水稻株高比野生型减少56.59%,有效分蘖数高出47.44%,差异均达到极显著水平。突变体的表皮毛消失且花发育迟缓,雄蕊变小。尽管突变体分蘖数较高但结实率明显降低,仅为野生型的12.62%,且种子长度和宽度均减小,差异极显著。通过显微镜观察茎的纵切切片,发现突变体细胞长度减少23%,差异极显著。外源喷施赤霉素后突变体的株高、有效分蘖、结实率、种子大小、表皮毛和茎秆细胞长度均有不同程度的恢复,说明植物体内赤霉素合成不足可能是引起水稻矮化的主要原因。转录组测序结果显示突变体中OsGA13ox 显著上调,qRT-PCR验证结果与转录组测序结果一致。由于OsGA13ox控制GA12转化为GA53,而GA12和GA53分别转化为GA4和GA1,GA4的活性高于GA1,因此,突变体中GA4减少可能是导致半矮化的主要原因。赤霉素检测结果表明突变体中GA4含量减少94.9%,与预测结果一致。此外,D14作为SL（独脚金内酯）的特异性受体,参与调控植物SL信号转导,抑制枝条分枝或者分蘖。qRT-PCR结果显示,与野生型相比,突变体中D14 显著下调,而经过GA3处理的野生型和突变体中D14 均显著上调。D14 上调可能导致有效分蘖数减少,而其下调可能致使有效分蘖数增加。统计结果表明突变体中有效分蘖显著增多,而经过GA处理之后,野生型和突变体有效分蘖数均显著低于未经GA3处理前,表明D14 在水稻中的表达可能受到GA的调控从而影响水稻分蘖。【结论】 OsGA13ox 异常表达导致活性更高的GA4在水稻中的富集减少,形成水稻半矮化突变体;赤霉素可能通过影响D14 的表达间接调控水稻的分蘖。

关键词: 水稻, 半矮化突变体, OsGA13ox, 赤霉素GA4, D14, 有效分蘖

Abstract:

【Objective】A dwarf rice mutant was generated by culturing rice embryo tissues and characterized to elucidate the reasons for leading to an occurence of semi-dwarf rice with more tiller number. It is expected that this study will provide a theoretical basis for scientifically cultivating rice varieties of lodging-resistant and high-yielding in overcoming the dwarf obstacle factors.【Method】In this study, both the rice dwarf mutant and wild type were phenotypically profiled, and the structural characteristics of flower and cell appearance of leaves were investigated by stereomicroscope and light microscopy; The differential gene expression profiles were analyzed by both the transcriptomics and qRT-PCR, and the sensitivity of the mutant to exogenous gibberellin was detected by spraying exogenous GA3; The enrichment profiles of gibberellin in the mutant were detected by a high performance liquid chromatography and a mass spectrometry. 【Result】Data showed that the mutant demonstrated a decrease of 56.59% in the average plant height and an increase of 47.44% in the effective tiller number compared with the wild type, respectively (P <0.01). Observation showed that the mutant led to disappearance of the epidermis and revealed a smaller stamen accompanying a delayed development in the flower organs. Although the mutant has a higher effective tiller number, but significantly lowers the seed setting rate, which only accounts for 12.62% of that in the wild type. The length and the width of grains also are significantly reduced (P <0.01). Stem longitudinal sections reveal that the mutant decreased the cell length of 23% compared with the wild type (P <0.01). However, when the mutant was exposed to exogenous gibberellin, the plant height, effective tiller number, seed setting rate, seed size, epidermis and the stem cell length were obviously restored, indicating that the dwarf mutant possibly results from the shortage of GA’s synthesis in plant. Transcriptome sequencing showed that the mutant significantly up-regulated the OsGA13ox gene, and exhibited an identical result with the qRT-PCR analyses. Since the OsGA13ox controls the conversion of the GA12 to the GA53, both of which are converted to the GA4 and the GA1, respectively. Particularly, the GA4 exhibits a higher activity than the GA1, suggesting that rice dwarf might be triggered by the reduction of GA4 enrichment in plant. Measurement confirmed that the accumulation of the GA4 in the mutant was decreased by 94.9% compared with the wild type. Additionally, as a specific receptor for SL (Strigolactone), the D14 gene is involved in the SL signaling transduction in plant, and thus inhibits branching or tillering. The qRT-PCR showed that the mutant significantly down-regulated the D14 gene compared to the wild type, however, both the wild type and the mutant significantly up-regulated after spraying GA3. The data suggested that the up-regulation of the D14 gene might lower the effective tiller number, while the down-regulation of the D14 gene possibly increase the effective tiller number. Statistical analyses demonstrates that the mutant significantly increased the effective tiller number, but both the wild type and mutant decreased the effective tiller number after spraying GA3, indicating that the expression profiles of the D14 gene in rice might be modulated by GA, and thereby exert on the tiller number.【Conclusion】Semi-dwarfed rice mutant is likely caused by a decrease of GA4 enrichment because of abnormal expression of the OsGA13ox gene, and GA might indirectly regulate rice tiller by affecting the expression of the D14 gene.

Key words: rice, semi-dwarf mutant, OsGA13ox, GA4, D14, effective tiller

黄升财,王冰,谢国强,刘中来,张美娟,张树清,程宪国. 赤霉素GA4是水稻矮化特征的重要调节因子[J]. 中国农业科学, 2019, 52(5): 786-800.

HUANG ShengCai,WANG Bing,XIE GuoQiang,LIU ZhongLai,ZHANG MeiJuan,ZHANG ShuQing,CHENG XianGuo. Enrichment Profile of GA4 is an Important Regulatory Factor Triggering Rice Dwarf[J]. Scientia Agricultura Sinica, 2019, 52(5): 786-800.

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基因 Gene	引物序列 Primer sequence (5′-3′)
β-Actin	F: CTGACAGGATGAGCAAGGAG R: GGCAATCCACATCTGCTGGA
OsGA13ox	F: AGAAGTGGAGAAAAGACTACGG R: CAATGATCTTTCTCTGGTGTGC
D14	F: AGAAAGAGAGAGAAGAAGCGAG R: CGCGCTCCCCTTTTATATACTA

基因 Gene	功能 Function
Os01g0115600	蛋白激酶功能,结合ATP Kinase function, binding with ATP
Os01g0781700	ATP结合功能ATP binding
Os10g0463800	功能未知Unknown function
Os03g0332100	GA13ox,催化GA12转化为GA53,决定GA4和GA1的比例^[24] GA13ox, Converts GA12 into GA53, determines the ratio of GA4 and GA1^[24]
Os04g0223500	黄素腺嘌呤二核苷酸,单加氧酶活性 FAD, monooxygenase activity
Os04g0606000	酰基转移酶活性 Transferase activity, transferring acyl groups
Os06g0364500	多糖结合功能Polysaccharide binding
Os12g0231700	推测是转位子蛋白Transposon protein, putative

基因 Gene	功能 Function
Novel00727	Lnc RNA,可能参与调控植物的表型Lnc RNA, probably participate in regulating the phenotype of plants
Os01g0191200	磷酸激酶活性 Acid phosphatase activity
Os01g0198900	功能未知 Unknow function
Os01g0368900	谷胱甘肽-二硫化物氧化还原酶活性 Has a glutathione-disulfide oxidoreductase activity
Os01g0501800	参与光合系统Ⅱ的组装和稳定 Involved in photosystem II assembly and stabilization
Os01g0600900	光受体,捕捉光信号 The light-harvesting complex functions as a light receptor
Os01g0948200	转录因子 DNA-binding transcription factor activity
Os03g0159900	功能未知 Unknow function
Os03g0196250	功能未知 Unknow function
Os03g0203200	D14蛋白参与独脚金内酯（SL）信号通路;与赤霉素竞争DELLA结合位点^[27] Involved in strigolactone signaling pathway; Competition with GA for DELLA binding sites^[27]
Os03g0251350	转录因子,调控长日照开花^[28]Probable transcription factor involved in the regulation of flowering time under long day^[28]
Os03g0856500	与核糖体小亚基结合,负调控蛋白翻译 Ribosomal small subunit binding, negative regulation of translational elongation

基因 Gene	基序 Motif	家族 Family	功能 Function
Os03g0224200	CAGTATCTTT	ARR-B	参与细胞分裂素信号的转导^[39] Participation in the transduction of cytokinin signaling^[39]
Os06g0130600	CACTCACATTCTCTCTGCACA	BBR-BPC	调控大麦BKn3 的表达^[40] Regulate the expression of BKn3 gene in barley^[40]
Os10g0419200	TTTTTTTTTTTG	C2H2	促进植物营养生长到生殖生长的转变^[41] Promote the transformation of plant vegetative growth to reproductive growth^[41]
Os07g0236700	GTATCTTTTTTTTTTTGCGCC	Dof^[42]	可能参与碳水化合物代谢基因的光调节,植物防御机制 Possible involvement in light regulation of carbohydrate metabolism genes, plant defense mechanisms
Os03g0821200	AACAAAGCTAAAACATAAGGG	Dof^[42]	参与种子萌发,发芽后糊粉中的赤霉素反应等基因的调控 Involving in seed germination, regulation of gibberellin reactions in aleurone after germination
Os03g0324300	GTCTAGATTCATTAC	EIL	乙烯信号响应的正向调控因子^[43] Positive regulator of ethylene signal response^[43]
Os03g0324200	GTCTAGATTCATTAC	EIL	在植物受伤时,参与乙烯信号的转导^[44] Participation in the transduction of ethylene signals when plants are injured^[44]
Os09g0490200	TAATGAATCTA	EIL	参与乙烯信号转导 Participation in ethylene signal transduction
Os11g0681200	GAAACACGCGCGCGC	FAR1	参与抵抗疾病^[45] Enhance the resistance of disease^[45]
Os02g0680700	TAGATATTA	MYB_related^[46]	功能未知 Unknown function
Os08g0157600	TAGATATTA	MYB_related^[46]	功能未知 Unknown function