关键词: 葡萄, 根系, 镉胁迫, 水杨酸, ATPase

Abstract:

【Objective】 To explore the effect of salicylic acid (SA) on cadmium stress and the pathway of alleviating cadmium damage to grapes roots. 【Method】 The experiment was carried out with the cutting seedlings of Zexiang grape pretreated by salicylic acid under CdCl2 stress via hydroponics. The activity of root, the activity of H+-ATPase and Ca2+-ATPase in plasma membrane (PM), and the formation of reactive oxygen species and nitric oxide (NO) were determine. 【Result】 The 0.01 mmol•L-1 CdCl2 increased the activity of root and PM Ca2+-ATPase. 1.0 mmol•L-1 CdCl2 promoted the formation of , H2O2 and NO, and inhibited significantly root activity and the activity of H+-ATPase and Ca2+-ATPase in PM. Pretreatment of SA at 50 μmol•L-1 greatly decreased the formation of , H2O2 and NO, which retarded the declining of root activity and the activity of H+-ATPase and Ca2+-ATPase in PM under 1.0 mmol•L-1 CdCl2 treatment. This effect of SA on roots decreased when SA concentration was raised to 200 μmol•L-1. 【Conclusion】It was found that SA at 50 μmol•L-1 is the optimal concentration for Alleviation of the damage of cadmium on grape roots, and at this concentration, SA mitigated the injurious effects of cadmium on root activity and PM ATPase in roots of grape by inhibiting the formation of free radicals.

Key words: grape, roots, cadmium stress, salicylic acid, ATPase