不同锌水平下GFabV对‘阳光玫瑰’葡萄光合效率及光合相关基因表达的影响

doi:10.3864/j.issn.0578-1752.2025.24.007

摘要/Abstract

摘要：

【目的】研究不同锌水平下GFabV对‘阳光玫瑰’葡萄光合特性的影响，通过分析光合作用相关基因相对表达差异，阐明GFabV影响叶片光合效率的分子机理。【方法】以携带GFabV‘阳光玫瑰’葡萄为试材、以无病毒苗为对照分别进行霍格兰营养液锌充足（0.22 mg·L^-1）和锌缺乏（0）处理。随后观察叶片症状表现；测定叶片锌含量、GFabV表达量、叶片光合参数、叶绿素荧光参数和叶绿素含量；筛选与光合作用相关的差异表达基因，并对关键基因表达水平进行验证。【结果】在锌缺乏条件下，带GFabV和无病毒植株锌含量分别为1.79和9.59 mg·kg^-1；锌充足条件下含量分别为18.84和23.16 mg·kg^-1。锌缺乏时带GFabV植株叶片表现皱缩小叶、轻微褪绿症状，GFabV表达量显著高于锌充足处理；锌充足时带GFabV植株叶片未表现症状；锌缺乏时无病毒植株叶片表现轻微褪绿症状。与锌充足条件下无病毒植株相比，带GFabV植株、锌缺乏无病毒植株叶片的叶绿素a、叶绿素b、叶绿素a+b、PSII最大光化学效率（Fv/Fm）和PSII实际光化学效率（ΦPSII）均显著下降，以锌缺乏时带GFabV植株叶片叶绿素（a、b、a+b）含量、Fv/Fm、ΦPSII最低，分别为1.22、0.35、1.57 mg·g^-1、0.40和0.04；带GFabV植株、锌缺乏下无病毒植株叶片净光合速率（Pn）、蒸腾速率（Tr）和气孔导度（Gs）均显著下降，以锌缺乏时带GFabV植株下降幅度最大，分别降至锌充足无病毒处理植株的16.27%、20.08%和21.99%。转录组测序和RT-qPCR结果表明，与锌充足下无病毒植株相比，带GFabV植株在锌缺乏时叶片PsbP、petC、PSBO和psaD表达量分别降至41.50%、54.81%、41.04%和44.45%；在锌充足时叶片ATPD、SEND33、psbA表达量显著降低；无病毒植株在锌缺乏时叶片PPD4、psbA表达量显著降低。与锌充足下带GFabV植株相比，在锌缺乏下带GFabV植株叶片PsbP、PSBO和psaD表达量分别降至55.03%、43.26%和53.36%。【结论】携带GFabV‘阳光玫瑰’葡萄叶片在锌缺乏条件下叶绿素含量显著降低，GFabV表达量升高，叶片出现皱缩小叶、轻微褪绿症状。GFabV通过下调叶片中PsbP、PSBO和psaD等光合相关基因表达，导致光合参数、叶绿素荧光参数显著下降影响叶片光合效率。

关键词: ‘阳光玫瑰’葡萄, 葡萄蚕豆萎蔫病毒, 锌缺乏, 基因表达量, 光合特性, 光合相关基因

Abstract:

【Objective】This study aimed to explore the effects of GFabV on photosynthetic characteristics in ‘Shine Muscat’ grapevines under different Zn levels, and to elucidate the molecular mechanism of GFabV affecting leaf photosynthetic efficiency by analyzing the relative expression differences of photosynthesis-related genes.【Method】‘Shine Muscat’ grapevines infected with GFabV were treated with Zn-sufficient (0.22 mg·L^-1) and Zn-deficient (0) Hoagland nutrient solution, respectively, and virus-free plants were used as control. The symptom of grapevine leaves was observed, and Zn content, GFabV expression level, photosynthetic parameters, chlorophyll fluorescence parameters, and chlorophyll content in the leaves were measured. Differentially expressed genes (DEGs) related to photosynthesis were screened, and expression level of key genes was verified.【Result】Under Zn deficiency, the Zn contents in the leaves of plants infected with GFabV and virus-free plants were 1.79 and 9.59 mg·kg^-1, respectively, and the Zn contents of those under Zn sufficiency were 18.84 and 23.16 mg·kg^-1, respectively. The leaves of plants infected with GFabV showed shrinkage leaflets, and slight chlorosis symptoms under Zn deficiency, and GFabV relative expression was significantly higher than that of the leaves infected with GFabV under Zn sufficiency. The leaves of plants infected with GFabV did not show symptoms under Zn sufficiency. The leaves of virus-free plants showed slight chlorotic symptoms under Zn deficiency. In comparison to the virus-free plants treated with Zn sufficiency, the contents of Chla, Chlb, Chla+b, Fv/Fm and ΦPSII in leaves of the plants infected with GFabV and the virus-free plants under Zn deficiency were significantly decreased. The chlorophyll (a, b, a+b) contents, Fv/Fm and ΦPSII in the leaves of the plants infected with GFabV under Zn deficiency were the lowest, which were 1.22, 0.35, 1.57 mg·g^-1, 0.40 and 0.04, respectively. The net photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (Gs) of the plants infected with GFabV and the virus-free plants under Zn deficiency were significantly lower than those of the virus-free plants under Zn sufficiency. The Pn, Tr, and Gs in the leaves of plants infected with GFabV under Zn deficiency decreased to 16.27%, 20.08% and 21.99% of that in the leaves of the virus-free plants under Zn sufficiency, respectively. Transcriptome sequencing and RT-qPCR results showed that the expression levels of PsbP, petC, PSBO, and psaD in the leaves infected with GFabV under Zn deficiency decreased to 41.50%, 54.81%, 41.04% and 44.45%, respectively, compared with those of the virus-free plants under Zn sufficiency. The expression levels of ATPD, SEND33, and psbA in the leaves were significantly decreased under Zn sufficiency. The expression levels of PPD4 and psbA in the leaves of virus-free plants were significantly decreased under Zn deficiency. The expression levels of PsbP, PSBO, and psaD in leaves infected with GFabV under Zn deficiency decreased to 55.03%, 43.26% and 53.36%, respectively, compared with those of the leaves infected with GFabV under Zn sufficiency.【Conclusion】The chlorophyll content of ‘Shine Muscat’ grapevine leaves carrying GFabV was significantly reduced, and the expression level of GFabV was increased under Zn deficiency. The leaves showed shrinkage leaflets and slight chlorosis symptoms. GFabV down-regulated photosynthesis-related genes expression such as PsbP, PSBO, and psaD, resulting in a significant decrease in photosynthetic parameters and chlorophyll fluorescence parameters in leaves, which affected the photosynthetic efficiency of leaves.

Key words: ‘Shine Muscat’ grapevine, grapevine fabavirus (GFabV), Zn deficiency, gene expression level, photosynthetic characteristics, photosynthesis-related gene

张向昆, 李佳莹, 乔如梦, 何静蕾, 王莉, 师校欣, 杜国强. 不同锌水平下GFabV对‘阳光玫瑰’葡萄光合效率及光合相关基因表达的影响[J]. 中国农业科学, 2025, 58(24): 5190-5200.

ZHANG XiangKun, LI JiaYing, QIAO RuMeng, HE JingLei, WANG Li, SHI XiaoXin, DU GuoQiang. Effects of GFabV Under Different Zn Levels on Photosynthetic Efficiency and Photosynthesis-Related Gene Expression of ‘Shine Muscat’ Grapevine[J]. Scientia Agricultura Sinica, 2025, 58(24): 5190-5200.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2025.24.007

https://www.chinaagrisci.com/CN/Y2025/V58/I24/5190

图/表 9

表1

表2

图1

图2

图3

表3

图4

表4

图5

参考文献 42

[1]	王令宇, 杨毓贤, 叶东东, 葛孟清, 诸葛雅贤, 王霏, 张晓雯, 肖鑫, 余文斌, 刘畅, 房经贵, 上官凌飞. 我国阳光玫瑰葡萄果实品质性状调查分析. 山西农业科学, 2022, 50(7): 1009-1015.
	WANG L Y, YANG Y X, YE D D, GE M Q, ZHUGE Y X, WANG F, ZHANG X W, XIAO X, YU W B, LIU C, FANG J G, SHANGGUAN L F. Investigation and analysis of fruit quality traits of Shine Muscat grape in China. Journal of Shanxi Agricultural Sciences, 2022, 50(7): 1009-1015. (in Chinese)
[2]	何晶晶, 李胜, 王荣, 许延帅, 陈文婷, 王美军, 杨国顺, 白描. ‘阳光玫瑰’葡萄病毒病症状差异及其对品质的影响. 中外葡萄与葡萄酒, 2024(3): 47-52.
	HE J J, LI S, WANG R, XU Y S, CHEN W T, WANG M J, YANG G S, BAI M. Difference of symptoms of grape virus disease and its influence on quality of ‘Shine Muscat’. Sino-Overseas Grapevine & Wine, 2024(3): 47-52. (in Chinese)
[3]	范旭东, 董雅凤, 张尊平, 张梦妍, 任芳, 胡国君. ‘阳光玫瑰’葡萄病毒小RNA测序鉴定及RT-PCR检测. 植物病理学报, 2019, 49(6): 749-755. doi: 10.13926/j.cnki.apps.000396
	FAN X D, DONG Y F, ZHANG Z P, ZHANG M Y, REN F, HU G J. Small RNA sequencing and RT-PCR detection of viruses infecting ‘Shine Muscat’ grapevines. Acta Phytopathologica Sinica, 2019, 49(6): 749-755. (in Chinese)
[4]	TARQUINI G, ZAINA G, ERMACORA P, DE AMICIS F, FRANCO- OROZCO B, LOI N, MARTINI M, BIANCHI G L, PAGLIARI L, FIRRAO G, DE PAOLI E, MUSETTI R. Agroinoculation of grapevine pinot gris virus in tobacco and grapevine provides insights on viral pathogenesis. PLoS ONE, 2019, 14(3): e0214010. doi: 10.1371/journal.pone.0214010
[5]	CUI Z H, BI W L, HAO X Y, XU Y, LI P M, WALKER M A, WANG Q C. Responses of in vitro-grown plantlets (Vitis vinifera) to grapevine leafroll-associated virus-3 and PEG-induced drought stress. Frontiers in Physiology, 2016, 7: 203.
[6]	MORINA F, MISHRA A, MIJOVILOVICH A, MATOUŠKOVÁ Š, BRÜCKNER D, ŠPAK J, KÜPPER H. Interaction between Zn deficiency, toxicity and turnip yellow mosaic virus infection in Noccaea ochroleucum. Frontiers in Plant Science, 2020, 11: 739. doi: 10.3389/fpls.2020.00739
[7]	AL RWAHNIH M, ALABI O J, WESTRICK N M, GOLINO D, ROWHANI A. Near-complete genome sequence of grapevine fabavirus, a novel putative member of the genus Fabavirus. Genome Announcements, 2016, 4(4): e00703-16.
[8]	FAN X D, ZHANG Z P, REN F, HU G J, LI Z N, DONG Y F. First report of grapevine fabavirus in grapevines in China. Plant Disease, 2017, 101(5): 847.
[9]	JO Y, SONG M K, CHOI H, PARK J S, LEE J W, CHO W K. First report of grapevine fabavirus in diverse Vitis species in Korea. Plant Disease, 2017, 101(10): 1829.
[10]	AL RWAHNIH M, ALABI O J, WESTRICK N M, GOLINO D, ROWHANI A. Description of a novel monopartite geminivirus and its defective subviral genome in grapevine. Phytopathology, 2017, 107: 240-251. doi: 10.1094/PHYTO-07-16-0282-R pmid: 27670772
[11]	CHIAKI Y, ITO T, SATO A, SUGIURA H, NISHIMURA R. Dwarfing caused by viral pathogens and leaf malformations in ‘Shine Muscat’ grapevine. Journal of General Plant Pathology, 2020, 86(1): 34-38. doi: 10.1007/s10327-019-00888-0
[12]	沈喜, 李红玉. 病毒侵染后植物叶绿体光合作用变化的分子机制. 植物病理学报, 2003, 33(4): 289-291.
	SHEN X, LI H Y. The molecular mechanism of plant chlorophyll photosynthetic changes after virus infection. Acta Phytopathologica Sinica, 2003, 33(4): 289-291. (in Chinese)
[13]	张梦妍. 葡萄蚕豆萎蔫病毒荧光定量PCR检测及对寄主的影响研究[D]. 北京: 中国农业科学院, 2020.
	ZHANG M Y. Detection of grapevine fabavirus by quantitative PCR and its effect on host[D]. Beijing: Chinese Academy of Agricultural Sciences, 2020. (in Chinese)
[14]	GAMBINO G, CUOZZO D, FASOLI M, PAGLIARANI C, VITALI M, BOCCACCI P, PEZZOTTI M, MANNINI F. Co-evolution between grapevine rupestris stem pitting-associated virus and Vitis vinifera L. leads to decreased defence responses and increased transcription of genes related to photosynthesis. Journal of Experimental Botany, 2012, 63(16): 5919-5933. doi: 10.1093/jxb/ers244
[15]	BERTAMINI M, MUTHUCHELIAN K, NEDUNCHEZHIAN N. Effect of grapevine leafroll on the photosynthesis of field grown grapevine plants (Vitis vinifera L. cv. Lagrein). Journal of Phytopathology, 2004, 152(3): 145-152. doi: 10.1111/jph.2004.152.issue-3
[16]	ZHANG B D, ZHANG M Y, JIA X J, HU G J, REN F, FAN X D, DONG Y F. Integrated transcriptome and metabolome dissecting interaction between Vitis vinifera L. and grapevine fabavirus. International Journal of Molecular Sciences, 2023, 24(4): 3247. doi: 10.3390/ijms24043247
[17]	SONG Y S, HANNER R H, MENG B Z. Transcriptomic analyses of grapevine leafroll-associated virus 3 infection in leaves and berries of ‘Cabernet Franc’. Viruses, 2022, 14(8): 1831. doi: 10.3390/v14081831
[18]	李惠, 刘利, 李秀杰, 孙庆华, 韩燕, 朱自果, 韩真, 李勃. 盐碱胁迫对‘阳光玫瑰’葡萄氮素吸收利用的影响. 中外葡萄与葡萄酒, 2024(6): 32-39.
	LI H, LIU L, LI X J, SUN Q H, HAN Y, ZHU Z G, HAN Z, LI B. Effects of salt-alkali stress on nitrogen absorption and utilization in ‘Shine Muscat’ grapevine. Sino-Overseas Grapevine & Wine, 2024(6): 32-39. (in Chinese)
[19]	王佳悦, 李光宗, 李娟, 单守明, 李翔. 水分胁迫对北红葡萄果实品质及有机酸合成基因表达的影响. 果树学报, 2025, 42(2): 266-275.
	WANG J Y, LI G Z, LI J, SHAN S M, LI X. Effects of water stress on berry quality and organic acid synthesis gene expression in Beihong grape. Journal of Fruit Science, 2025, 42(2): 266-275. (in Chinese)
[20]	付伟. 锌肥对‘阳光玫瑰’葡萄叶片光合特性和果实品质的影响研究[D]. 雅安: 四川农业大学, 2019.
	FU W. Effects of zinc fertilizer on photosynthetic characteristics of leaves and fruit quality of ‘Shine Muscat’ grape[D]. Yaan: Sichuan Agricultural University, 2019. (in Chinese)
[21]	EL AOU-OUAD H, POU A, TOMÁS M, MONTERO R, RIBAS- CARBO M, MEDRANO H, BOTA J. Combined effect of virus infection and water stress on water flow and water economy in grapevines. Physiologia Plantarum, 2017, 160(2): 171-184. doi: 10.1111/ppl.2017.160.issue-2
[22]	崔振华. 葡萄试管苗对葡萄卷叶病和PEG诱导的干旱胁迫的响应及耐病毒砧木评价的研究[D]. 杨凌: 西北农林科技大学, 2017.
	CUI Z H. Responses of in vitro grapevine plantlets to grapevine leafroll-associated virus infection and PEG-induced drought stress, and preliminary evaluations of tolerance of grapevine rootstocks to grapevine leafroll disease[D]. Yangling: Northwest A&F University, 2017. (in Chinese)
[23]	BUOSO S, PAGLIARI L, MUSETTI R, FORNASIER F, MARTINI M, LOSCHI A, FONTANELLA M C, ERMACORA P. With or without you: Altered plant response to boron-deficiency in hydroponically grown grapevines infected by grapevine pinot gris virus suggests a relation between grapevine leaf mottling and deformation symptom occurrence and boron plant availability. Frontiers in Plant Science, 2020, 11: 226. doi: 10.3389/fpls.2020.00226 pmid: 32194603
[24]	HAO X Y, JIAO B L, LIU Z M, WANG X W, WANG J Y, ZHANG J X, WANG Q C, XU Y, WANG Q C. Crosstalk between grapevine leafroll-associate virus-3 (GLRaV-3) and NaCl-induced salt stress in in vitro cultures of the red grape ‘Cabernet Sauvignon’. Plant Cell, Tissue and Organ Culture, 2021, 144(3): 649-660. doi: 10.1007/s11240-020-01987-z
[25]	FAN X D, ZHANG M Y, ZHANG Z P, REN F, HU G J, DONG Y F. Prevalence and genetic diversity of grapevine fabavirus isolates from different grapevine cultivars and regions in China. Journal of Integrative Agriculture, 2020, 19(3): 768-774. doi: 10.1016/S2095-3119(19)62677-8
[26]	AHMED N, ZHANG B G, CHACHAR Z, LI J, XIAO G S, WANG Q, HAYAT F, DENG L S, NAREJO M, BOZDAR B, TU P F. Micronutrients and their effects on horticultural crop quality, productivity and sustainability. Scientia Horticulturae, 2024, 323: 112512. doi: 10.1016/j.scienta.2023.112512
[27]	郭世荣. 无土栽培学. 北京: 中国农业出版社, 2003: 98-112.
	GUO S R. Soilless Culture. Beijing: China Agriculture Press, 2003: 98-112. (in Chinese)
[28]	王学奎. 植物生理生化实验原理和技术. 北京: 高等教育出版社, 2006: 134-136.
	WANG X K. Principles and Techniques of Plant Physiological Biochemical Experiment. Beijing: Higher Education Press, 2006: 134-136. (in Chinese)
[29]	马宗桓, 高静涛, 边志远, 陈佰鸿, 毛娟, 郭艳兰, 陈胜, 梁国平, 李蔚, 赵彦红. 葡萄叶片营养诊断技术规程: DB62/T 5049-2024[S]. (2024-11-20) [2025-08-17].
	MA Z H, GAO J T, BIAN Z Y, CHEN B H, MAO J, GUO Y L, CHEN S, LIANG G P, LI W, ZHAO Y H. Technical specification for nutrition diagnosis of grape leaves: DB62/T 5049-2024[S]. (2024-11- 20) [2025-08-17]. (in Chinese)
[30]	余茜. 不同苹果品种锌含量差异及‘长富2号’苹果补锌措施研究[D]. 杨凌: 西北农林科技大学, 2024.
	YU Q. Study on the differences of zinc content in different apple varieties and the method of supplementing zinc in ‘Nagano Fuji No.2’[D]. Yangling: Northwest A&F University, 2024. (in Chinese)
[31]	ZANINI A, DI FEO L, LUNA D F, PACCIORETTI P, COLLAVINO A, RODRIGUEZ M S. Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants. Plant Pathology, 2021, 70(1): 195-205. doi: 10.1111/ppa.v70.1
[32]	孙明飞, 朱杰, 李潞, 高美娜, 梁博文, 周莎莎, 徐继忠, 李中勇. 缺锌胁迫对‘天红2号/冀砧2号’苹果幼树生长、光合特性和内源激素含量的影响. 植物营养与肥料学报, 2023, 29(3): 544-552.
	SUN M F, ZHU J, LI L, GAO M N, LIANG B W, ZHOU S S, XU J Z, LI Z Y. Effects of zinc deficiency on growth, photosynthetic characteristics, and endogenous hormone content of apple sapling ‘Tianhong 2/Jizhen 2’. Journal of Plant Nutrition and Fertilizers, 2023, 29(3): 544-552. (in Chinese)
[33]	GRISHAM M P, JOHNSON R M, ZIMBA P V. Detecting sugarcane yellow leaf virus infection in asymptomatic leaves with hyperspectral remote sensing and associated leaf pigment changes. Journal of Virological Methods, 2010, 167(2): 140-145. doi: 10.1016/j.jviromet.2010.03.024 pmid: 20362003
[34]	MARTINELLI F, SCALENGHE R, DAVINO S, PANNO S, SCUDERI G, RUISI P, VILLA P, STROPPIANA D, BOSCHETTI M, GOULART L, DAVIS C E, DANDEKAR A M. Advanced methods of plant disease detection. A review. Agronomy for Sustainable Development, 2015, 35: 1-25. doi: 10.1007/s13593-014-0246-1
[35]	HUSEYNOVA I M, MIRZAYEVA S M, SULTANOVA N F, ALIYEVA D R, MUSTAFAYEV N S, ALIYEV J A. Virus-induced changes in photosynthetic parameters and peroxidase isoenzyme contents in tomato (Solanum lycopersicum L.) plants. Photosynthetica, 2018, 56: 841-850. doi: 10.1007/s11099-017-0737-9
[36]	BIANCHI G L, AMICIS F, SABBATA L, BERNARDO N, GOVERNATORI G, NONINO F, PRETE G, MARRAZZO T, VERSOLATTO S, FRAUSIN C. Occurrence of grapevine pinot gris virus in Friuli Venezia Giulia (Italy): Field monitoring and virus quantification by real-time RT-PCR. EPPO Bulletin, 2015, 45(1): 22-32. doi: 10.1111/epp.2015.45.issue-1
[37]	SHAFIQ M, IQBAL Z, ALI I, ABBAS Q, MANSOOR S, BRIDDON R W, AMIN I. Real-time quantitative PCR assay for the quantification of virus and satellites causing leaf curl disease in cotton in Pakistan. Journal of Virological Methods, 2017, 248: 54-60. doi: S0166-0934(16)30523-7 pmid: 28572041
[38]	付春霞, 张元珍, 王衍安, 范晓丹, 闫玉静, 张友朋. 缺锌胁迫对苹果叶片光合速率及叶绿素荧光特性的影响. 中国农业科学, 2013, 46(18): 3826-3833. doi:10.3864/j.issn.0578-1752.2013.18.011.
	FU C X, ZHANG Y Z, WANG Y A, FAN X D, YAN Y J, ZHANG Y P. Effects of zinc deficiency on photosynthetic rate and chlorophyll fluorescence characteristics of apple leaves. Scientia Agricultura Sinica, 2013, 46(18): 3826-3833. doi:10.3864/j.issn.0578-1752.2013.18.011. (in Chinese)
[39]	张莉, 任媛媛, 张岁岐. 锌缺乏对植物生长发育的影响. 现代农业研究, 2020, 26(5): 54-55.
	ZHANG L, REN Y Y, ZHANG S Q. Effects of zinc deficiency on plant growth and development. Modern Agriculture Research, 2020, 26(5): 54-55. (in Chinese)
[40]	BERTAMINI M, MALOSSINI U, MUTHUCHELIAN K, NEDUNCHEZHIAN N. Physiological response of field grown grapevine (Vitis vinifera L. cv. Marzernino) to grapevine leaf roll- associated virus (GLRaV-1). Phytopathologia Mediterranea, 2005, 44(3): 256-265.
[41]	QIU S, ZHANG Y J, SUN H C, LIU L T, LI C D, HUA Z Q, DONG H Z. Integrated transcriptomics and metabolomics elucidate additive inhibitory effects of combined salinity-waterlogging stress on soybean growth and metabolic adaptations. Plant Physiology and Biochemistry, 2025, 223: 109847. doi: 10.1016/j.plaphy.2025.109847
[42]	CHEN L J, SUN D L, ZHANG X X, SHAO D Q, LU Y L, AN Y X. Transcriptome analysis of yellow passion fruit in response to cucumber mosaic virus infection. PLoS ONE, 2021, 16(2): e0247127. doi: 10.1371/journal.pone.0247127

化合物 Compound	含量Content (mg·L^-1)
化合物 Compound	锌充足 Zn sufficiency	锌缺乏 Zn deficiency
Ca(NO₃)₂·4H₂O	945	945
KNO₃	607	607
NH₄H₂PO₄	115	115
MgSO₄·7H₂O	493	493
乙二胺四乙酸二钠铁NaFe-EDTA	40	40
硼酸H₃BO₃	2.86	2.86
MnSO₄·4H₂O	2.13	2.13
ZnSO₄·7H₂O	0.22	0
CuSO₄·5H₂O	0.08	0.08
钼酸铵(NH₄)₂Mo₇O₂₄·4H₂O	0.025	0.025

基因名称 Gene name	引物序列 Primer sequence (5′-3′)	产物大小 Product size (bp)
petC	F: GCCACCTATGGTATTAACGCGG R: GTCCTCTGACAACCCTGCCT	130
PSBO	F: TCGCTTGACCTACACCCTCG R: CCCGATACGAGGGGACCAAG	218
ATPD	F: AACGCAACGTTTTGGACGAG R: TGCTCCGACTCCAGTTTCAC	196
psaD	F: TGAGTTGGACCCCAATACGC R: GCACTGCTCTTTCCTAGCCA	196
PsbP	F: TTTCCAGTGCAGACAGCGTG R: TGTCTCGGGTCCATTCTGGT	174
SEND33	F: GTGAGCACCTCCTTCATCCG R: CCAAACAGAGCCTGACCCAC	77

处理 Treatment	叶绿素a含量 Chla content (mg·g^-1)	叶绿素b含量 Chlb content (mg·g^-1)	总叶绿素含量 Chl a+b content (mg·g^-1)	PSII最大光化学效率 Fv/Fm	PSII实际光化学效率 ΦPSII
GFabV+/Zn-	1.22±0.07c	0.35±0.06c	1.57±0.12c	0.40±0.02c	0.04±0.01c
GFabV+/Zn+	1.64±0.03b	0.64±0.01b	2.28±0.04b	0.58±0.08b	0.08±0.02b
GFabV-/Zn-	1.59±0.02b	0.63±0.03b	2.21±0.03b	0.60±0.09b	0.07±0.01b
GFabV-/Zn+	2.00±0.04a	0.80±0.05a	2.80±0.09a	0.75±0.03a	0.14±0.03a

基因ID Gene ID	基因名称 Gene name	Log₂FC
基因ID Gene ID	基因名称 Gene name	GFabV+/Zn- vs GFabV-/Zn+	GFabV-/Zn- vs GFabV-/Zn+	GFabV+/Zn+ vs GFabV-/Zn+	GFabV+/Zn- vs GFabV+/Zn+
LOC100252244	PPD4	-1.10	-0.64	-0.98	—
LOC132254568	psbA	-0.87	-0.87	-1.04	—
LOC100258958	ATPC	2.37	2.07	1.48	—
LOC100260811	PSBO	-1.09	—	—	-0.79
LOC100242600	psaD	-1.41	—	—	-1.08
LOC100261871	PsbP	-1.00	—	—	-0.61
LOC100245219	petC	-1.03	—	-0.66	—
LOC100251062	ATPD	-1.04	—	-0.56	—
LOC100249080	SEND33	-1.03	—	-0.74	—
LOC104878430	psbH	-3.64	—	—	-3.30