有机肥替代化肥与丛枝菌根真菌互作对砂姜黑土和潮土N2O排放的影响

doi:10.3864/j.issn.0578-1752.2025.01.008

中国农业科学 ›› 2025, Vol. 58 ›› Issue (1): 101-116.doi: 10.3864/j.issn.0578-1752.2025.01.008

• 土壤肥料·节水灌溉·农业生态环境 • 上一篇下一篇

有机肥替代化肥与丛枝菌根真菌互作对砂姜黑土和潮土N₂O排放的影响

杜嘉琪¹(), 张紫薇¹, 王若飞¹, 黎星¹, 郭红艳², 杨硕¹, 冯成¹, 何堂庆¹, Giri Bhoopander³, 张学林¹^,^*()

¹ 河南农业大学农学院/省部共建小麦玉米作物学国家重点实验室/教育部作物生长发育调控重点实验室，中国郑州 450046
² 商丘市经济作物技术推广中心，中国河南商丘 476000
³ 印度德里大学Swami Shraddhanand学院植物系，印度德里 110021

收稿日期:2024-01-11 接受日期:2024-03-11 出版日期:2025-01-01 发布日期:2025-01-07
通信作者:
张学林，E-mail：xuelinzhang1998@163.com
联系方式: 杜嘉琪，E-mail：438905552@qq.com。
基金资助:
国家自然科学基金面上项目(32371633); 河南农业大学科技创新基金(30500712)

The Interactive Effects of Organic Fertilizer Substituting Chemical Fertilizers and Arbuscular Mycorrhizal Fungi on Soil Nitrous Oxide Emission in Shajiang Black Soil and Fluvo-Aquic Soil

DU JiaQi¹(), ZHANG ZiWei¹, WANG RuoFei¹, LI Xing¹, GUO HongYan², YANG Shuo¹, FENG Cheng¹, HE TangQing¹, Giri Bhoopander³, ZHANG XueLin¹^,^*()

¹ College of Agronomy, Henan Agricultural University/State Key Laboratory of Wheat and Maize Crop Science/Key Laboratory of Crop Growth and Development Regulation, Ministry of Education, Zhengzhou 450046, China
² Shangqiu Economic Crop Technology Promotion Center, Shangqiu 476000, Henan, China
³ Department of Botany, Swami Shraddhanand College, University of Delhi, Delhi 110021, India

Received:2024-01-11 Accepted:2024-03-11 Published:2025-01-01 Online:2025-01-07

摘要/Abstract

摘要：

【背景】有机肥替代化肥（organic fertilizer substitution：OF）是一种有效的农田化肥减量策略，可以改变土壤有机碳与无机氮的比例，调节土壤氧化亚氮（N₂O）排放。丛枝菌根真菌（arbuscular mycorrhizal fungi, AMF）通过与大多数陆生植物共生增加植物对土壤养分的吸收，调控N₂O排放。【目的】探索不同土壤类型有机肥替代化肥比例及其与丛枝菌根真菌互作对土壤N₂O排放的影响，分析其作用机制，为农田化肥减施提供技术支撑。【方法】选用砂姜黑土和潮土两种土壤类型，在玉米生育期设置有机肥替代化肥（仅施化学氮肥180 kg N·hm^-2，0%OF；等氮有机肥替代25%化学氮肥，25%OF；等氮有机肥替代50%化学氮肥，50%OF）和AMF（不接种丛枝菌根真菌：M-；接种丛枝菌根真菌：M+）双因素试验，并设不施肥对照（CK），于玉米生育期测定植株生物量、氮素累积量、籽粒产量、土壤无机氮和N₂O排放通量，同时测定砂姜黑土反硝化相关功能基因nirK和nirS的丰度。【结果】砂姜黑土上有机肥替代化肥不同比例之间玉米产量无显著差异，而潮土的玉米产量随替代比例增加呈降低趋势。与0%OF处理相比，砂姜黑土25%OF和50%OF处理N₂O累积排放量分别减少17.6%和18%；而潮土分别减少13.5%和3.9%。与M-相比，M+处理能够增加玉米籽粒硝酸还原酶、亚硝酸还原酶、谷氨酰胺合成酶、谷氨酸合成酶和籽粒产量，同时减少N₂O排放量，砂姜黑土N₂O累积排放量减少26.5%—28.2%，潮土减少2.7%—13.5%，其中砂姜黑土和潮土的25%OFM+处理N₂O累积排放量分别减少34.6%和22.5%，而根系总长度、根系表面积、根体积、根直径均显著增加。相关分析表明，砂姜黑土和潮土N₂O排放均与土壤NH₄⁺-N、NO₃^--N呈正相关，与AMF侵染率呈负相关；砂姜黑土N₂O排放与nirK和nirS基因拷贝数呈正相关。【结论】不同土壤类型有机肥替代化肥并接种AMF均能降低N₂O排放，这种影响主要通过AMF侵染扩大玉米根系氮素吸收范围和调控土壤反硝化关键功能微生物表达而实现。其中有机肥替代 25%化肥（25%OF）并接种AMF是实现玉米稳产、减肥、减排较为合理的施肥方式。

关键词: N₂O排放, 有机肥替代, 丛枝菌根真菌, 反硝化微生物, 砂姜黑土, 潮土

Abstract:

【Background】Organic fertilizer substitution (OF) is an effective chemical fertilizer reduction strategy, which can change the ratio of soil organic carbon to inorganic nitrogen (N), so as to regulate nitrous oxide (N₂O) emission. Similarly, arbuscular mycorrhizal fungi (AMF) forms a symbiotic strategy with most terrestrial plants, increases plant soil nutrient uptake, and affects soil N₂O emission. However, the interactive effects of both OF and AMF on soil N₂O emissions are poorly understood, especially in different agricultural soil environments, and the mechanism of their interaction is also unclear. 【Objective】 This study aimed to explore the mechanical effects of both OF and AMF on soil N₂O emissions during maize growth periods in different soil types, so as to provide the appropriate methods to reduce chemical fertilizer application for farmland management.【Method】Taking Shajiang black soil (SJ) and Fluvo-aquic soils (CT) as research object, a two factor experiment with organic fertilizers replacing chemical fertilizers (0%OF: only chemical N fertilizer; 25%OF: equal N organic fertilizer replacing 25% chemical N fertilizer; 50%OF: equal N organic fertilizer replacing 50% chemical N fertilizer) and AMF (M+, inoculation of arbuscular mycorrhizal fungi; M-, no inoculation of arbuscular mycorrhizal fungi) was carried out in 2020, with a non-fertilization control (CK). The maize biomass, N accumulation, grain yield, soil inorganic N, and soil N₂O emission flux were measured during maize growth period in both the soil types, and a relative abundance of denitrification functional genes, such as nirK and nirS, was investigated too. 【Result】There was no significant difference in maize grain yield among different OF treatments under the conditions of Shajiang black soil, while grain yield showed decreasing tendency with the OFS ratio increasing in Fluvo-aquic soils. Compared with 0%OF treatment, the cumulative N₂O emissions under 25%OF and 50%OF treatments in Shajiang black soil reduced by 17.6% and 18%, respectively, and by 13.5% and 3.9% in the Fluvo-aquic soil. Compared with non-AMF (M-treatment), the presence of AMF (M+ treatment) increased maize grain nitrate reductase, nitrite reductase, glutamine synthase, glutamate synthase, and maize grain yield, while reduced soil N₂O emissions. The reduction of cumulative N₂O emissions in Shajiang black soil was by 26.5%-28.2%, and by 2.7%-13.5% in Fluvo-aquic soil; the reduction in case of 25% OFM+ treatment was 34.6% and 22.5% in Shajiang black soil and Fluvo-aquic soils respectively, while the root total length, root surface area, root volume and root diameter per plant increased significantly. Correlation analysis showed that N₂O emissions were positively related with soil NH₄⁺and NO₃^- under both Shajiang black soil and Fluvo-aquic soil conditions, while negatively related with AMF infection rate in both Shajiang black and Fluvo-aquic soils; whereas, the emissions were positively related with the copy numbers of nirK and nirS genes under Shajiang black soil conditions.【Conclusion】The replacing chemical fertilizers with organic fertilizers and inoculation with AMF could reduce N₂O emissions under different soil types. This interactive effect might be the result of expanding root N absorption area through AMF colonization, and by regulating the expression of key functional microorganisms in soil denitrification. Therefore, the study recommends replacement of 25% chemical fertilizer (OF25%) with organic fertilizers and inoculation with AMF could be an ideal fertilizer management method to maintain maize production stable and reduce chemical fertilizer application rate and greenhouse gas emissions.

Key words: N₂O emissions, organic fertilizer substitution, arbuscular mycorrhizal fungi, denitrifying microorganisms, Shajiang black soil, Fluvo-aguic soil

杜嘉琪, 张紫薇, 王若飞, 黎星, 郭红艳, 杨硕, 冯成, 何堂庆, Giri Bhoopander, 张学林. 有机肥替代化肥与丛枝菌根真菌互作对砂姜黑土和潮土N₂O排放的影响[J]. 中国农业科学, 2025, 58(1): 101-116.

DU JiaQi, ZHANG ZiWei, WANG RuoFei, LI Xing, GUO HongYan, YANG Shuo, FENG Cheng, HE TangQing, Giri Bhoopander, ZHANG XueLin. The Interactive Effects of Organic Fertilizer Substituting Chemical Fertilizers and Arbuscular Mycorrhizal Fungi on Soil Nitrous Oxide Emission in Shajiang Black Soil and Fluvo-Aquic Soil[J]. Scientia Agricultura Sinica, 2025, 58(1): 101-116.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2025.01.008

https://www.chinaagrisci.com/CN/Y2025/V58/I1/101

图/表 9

表1

图1

图2

图3

图4

图5

图6

表2

图7

参考文献 44

[1]	RAVISHANKARA A R, DANIEL J S, PORTMANN R W. Nitrous oxide (N₂O): The dominant ozone-depleting substance emitted in the 21st century. Science, 2009, 326(5949): 123-125.
[2]	谢瑞芝, 明博. 玉米生产系统对气候变化的响应与适应. 中国农业科学, 2021, 54(17): 3587-3591. doi: 10.3864/j.issn.0578-1752.2021.17.003.
	XIE R Z, MING B. Response and adaptation of maize production system to climate change. Scientia Agricultura Sinica, 2021, 54(17): 3587-3591. doi: 10.3864/j.issn.0578-1752.2021.17.003. (in Chinese)
[3]	AGUILERA E, LASSALETTA L, SANZ-COBENA A, GARNIER J, VALLEJO A. The potential of organic fertilizers and water management to reduce N₂O emissions in Mediterranean climate cropping systems. A review. Agriculture, Ecosystems and Environment, 2013, 164: 32-52.
[4]	XIAO L L, SUN Q B, YUAN H T, LIAN B. A practical soil management to improve soil quality by applying mineral organic fertilizer. Acta Geochimica, 2017, 36(2): 198-204.
[5]	SONG H, WANG J, ZHANG K, ZHANG M Y, HUI R, SUI T Y, YANG L, DU W B, DONG Z R. A 4-year field measurement of N₂O emissions from a maize-wheat rotation system as influenced by partial organic substitution for synthetic fertilizer. Journal of Environmental Management, 2020, 263: 110384.
[6]	HUANG R, WANG Y Y, GAO X S, LIU J, WANG Z F, GAO M. Nitrous oxide emission and the related denitrifier community: a short-term response to organic manure substituting chemical fertilizer. Ecotoxicology and Environmental Safety, 2020, 192: 110291.
[7]	卜容燕, 李敏, 韩上, 程文龙, 王慧, 孙志祥, 唐杉, 武际. 有机无机肥配施对双季稻轮作系统产量、温室气体排放和土壤养分的综合效应. 应用生态学报, 2021, 32(1): 145-153. doi: 10.13287/j.1001-9332.202101.023
	BU R Y, LI M, HAN S, CHENG W L, WANG H, SUN Z R, TANG S, WU J. Comprehensive effects of combined application of organic and inorganic fertilizer on yield, greenhouse gas emissions, and soil nutrient in double-cropping rice systems. Chinese Journal of Applied Ecology, 2021, 32(1): 145-153. (in Chinese)
[8]	HODGE A, CAMPBELL C D, FITTER A H. An arbuscular mycorrhizal fungus accelerates decomposition and acquires nitrogen directly from organic material. Nature, 2001, 413(6853): 297-299.
[9]	JIANG S T, AN X R, SHAO Y D, KANG Y L, CHEN T S, MEI X L, DONG C X, XU Y C, SHEN Q R. Responses of arbuscular mycorrhizal fungi occurrence to organic fertilizer: A meta-analysis of field studies. Plant and Soil, 2021, 469(1/2): 1-17.
[10]	LI Y J, ZHENG Q, YANG R, ZHUANG S, LIN W, LI Y Z. Evaluating microbial role in reducing N₂O emission by dual isotopocule mapping following substitution of inorganic fertilizer for organic fertilizer. Journal of Cleaner Production, 2021, 326: 129442.
[11]	BUCHEN C, LEWICKA-SZCZEBAK D, FLESSA H, WELL R. Estimating N₂O processes during grassland renewal and grassland conversion to maize cropping using N₂O isotopocules. Rapid Communications in Mass Spectrometry, 2018, 32(13): 1053-1067.
[12]	LIANG D, ROBERTSON G P. Nitrification is a minor source of nitrous oxide (N₂O) in an agricultural landscape and declines with increasing management intensity. Global Change Biology, 2021, 27(21): 5599-5613.
[13]	QIU Y P, JIANG Y, GUO L J, ZHANG L, BURKEY K O, ZOBEL R W, REBERG-HORTON S C, SHEW H D, HU S J. Shifts in the composition and activities of denitrifiers dominate CO₂ stimulation of N₂O emissions. Environmental Science and Technology, 2019, 53(19): 11204-11213.
[14]	谢军, 赵亚南, 陈轩敬, 李丹萍, 徐春丽, 王珂, 张跃强, 石孝均. 有机肥氮替代化肥氮提高玉米产量和氮素吸收利用效率. 中国农业科学, 2016, 49(20): 3934-3943. doi:10.3864/j.issn.0578-1752.2016.20.008.
	XIE J, ZHAO Y N, CHEN X J, LI D P, XU C L, WANG K, ZHANG Y Q, SHI X J. Nitrogen of organic manure replacing chemical nitrogenous fertilizer improve maize yield and nitrogen uptake and utilization efficiency. Scientia Agricultura Sinica, 2016, 49(20): 3934-3943. doi:10.3864/j.issn.0578-1752.2016.20.008. (in Chinese)
[15]	赵吉霞, 禹妍彤, 周芸, 李永梅, 范茂攀. 有机肥等氮替代化肥对玉米产量和氮素吸收利用效率的影响. 水土保持研究, 2022, 29(5): 374-381.
	ZHAO J X, YU Y T, ZHOU Y, LI Y M, FAN M P. Effect of organic manure replacing chemical nitrogenous fertilizer on main yield and nitrogen uptake and utilization efficiency. Research of Soil and Water Conservation, 2022, 29(5): 374-381. (in Chinese)
[16]	ZHANG X Y, FANG Q C, ZHANG T, MA W Q, VELTHOF G L, HOU Y, OENEMA O, ZHANG F S. Benefits and trade-offs of replacing synthetic fertilizers by animal manures in crop production in China: A meta-analysis. Global Change Biology, 2020, 26(2): 888-900. doi: 10.1111/gcb.14826 pmid: 31495039
[17]	LUO G W, LI L, FRIMAN V P, GUO J J, GUO S W, SHEN Q R, LING N. Organic amendments increase crop yields by improving microbe-mediated soil functioning of agroecosystems: A meta-analysis. Soil Biology and Biochemistry, 2018, 124: 105-115.
[18]	ZHANG Y R, NIE Y, LIU Y L, HUANG X C, YANG Y H, XIONG H, ZHU H Q, LI Y. Characteristics of greenhouse gas emissions from yellow paddy soils under long-term organic fertilizer application. Sustainability, 2022, 14(19): 12574.
[19]	孟磊, 蔡祖聪, 丁维新. 长期施肥对华北典型潮土N分配和N₂O排放的影响. 生态学报, 2008, 28(12): 6197-6203.
	MENG L, CAI Z C, DING W X. Effects of long-term fertilization on N distribution and N₂O emission in fluvo-aquci soil in North China. Acta Ecologica Sinica, 2008, 28(12): 6197-6203. (in Chinese)
[20]	JIA J X, LI B, CHEN Z Z, XIE Z B, XIONG Z Q. Effects of biochar application on vegetable production and emissions of N₂O and CH₄. Soil Science and Plant Nutrition, 2012, 58(4): 503-509.
[21]	GUI H, GAO Y, WANG Z H, SHI L L, YAN K, XU J C. Arbuscular mycorrhizal fungi potentially regulate N₂O emissions from agricultural soils via altered expression of denitrification genes. The Science of the Total Environment, 2021, 774: 145133.
[22]	ZHANG X L, QIU Y P, GILLIAM F S, GILLESPIE C J, TU C, REBERG-HORTON S C, HU S J. Arbuscular mycorrhizae shift community composition of N-cycling microbes and suppress soil N₂O emission. Environmental Science and Technology, 2022, 56(18): 13461-13472.
[23]	CHEN H, ZHOU J, LI B, XIONG Z Q. Yield-scaled N₂O emissions as affected by nitrification inhibitor and overdose fertilization under an intensively managed vegetable field: A three-year field study. Atmospheric Environment, 2019, 206: 247-257.
[24]	XU F, LIU Y L, DU W C, LI C L, XU M L, XIE T C, YIN Y, GUO H Y. Response of soil bacterial communities, antibiotic residuals, and crop yields to organic fertilizer substitution in North China under wheat-maize rotation. Science of the Total Environment, 2021, 785: 147248.
[25]	李晓立, 何堂庆, 张晨曦, 田明慧, 吴梅, 李潮海, 杨青华, 张学林. 等氮量条件下有机肥替代化肥对玉米农田温室气体排放的影响. 中国农业科学, 2022, 55(5): 948-961. doi:10.3864/j.issn.0578-1752. 2022.05.009.
	LI X L, HE T Q, ZHANG C X, TIAN M H, WU M, LI C H, YANG Q H, ZHANG X L. Effect of organic fertilizer replacing chemical fertilizers on greenhouse gas emission under the conditions of same nitrogen fertilizer input in maize farmland. Scientia Agricultura Sinica, 2022, 55(5): 948-961. doi:10.3864/j.issn.0578-1752.2022.05.009. (in Chinese)
[26]	温延臣, 张曰东, 袁亮, 李伟, 李燕青, 林治安, 赵秉强. 商品有机肥替代化肥对作物产量和土壤肥力的影响. 中国农业科学, 2018, 51(11): 2136-2142. doi:10.3864/j.issn.0578-1752.2018.11.011.
	WEN Y C, ZHANG Y D, YUAN L, LI W, LI Y Q, LIN Z, ZHAO B Q. Crop yield and soil fertility response to commercial organic fertilizer substituting chemical fertilizer. Scientia Agricultura Sinica, 2018, 51(11): 2136-2142. doi: 10.3864/j.issn.0578-1752.2018.11.011. (in Chinese)
[27]	刘占军, 谢佳贵, 张宽, 王秀芳, 侯云鹏, 尹彩侠, 李书田. 不同氮肥管理对吉林春玉米生长发育和养分吸收的影响. 植物营养与肥料学报, 2011, 17(1): 38-47.
	LIU J Z, XIE J G, ZHANG K, WANG X F, HOU Y P, YIN C X, LI S T. Maize growth and nutrient uptake as influenced by nitrogen management in Jilin province. Journal of Plant Nutrition and Fertilizers, 2011, 17(1): 38-47. (in Chinese)
[28]	米国华, 陈范骏, 吴秋平, 赖宁薇, 袁力行, 张福锁. 玉米高效吸收氮素的理想根构型. 中国科学:生命科学, 2010, 40(12): 1112-1116.
	MI G H, CHEN F J, WU Q P, LAI N W, YUAN L X, ZHANG F S. Ideotype root architecture for efficient nitrogen acquisition by maize in intensive cropping systems. Science China: Life Sciences, 2010, 53(12): 1369-1373. (in Chinese)
[29]	HATI K M, MANDAL K G, MISRA A K, GHOSH P K, BANDYOPADHYAY K K. Effect HATI K M, MANDAL K G, MISRA A K, GHOSH P K, BANDYOPADHYAY K K. Effect of inorganic fertilizer and farmyard manure on soil physical properties, root distribution, and water-use efficiency of soybean in Vertisols of central India. Bioresource Technology, 2006, 97(16): 2182-2188. doi: 10.1016/j.biortech.2005.09.033 pmid: 16289791
[30]	郭喜军, 谢军红, 李玲玲, 王嘉男, 康彩睿, 彭正凯, 王进斌, Setorkwami Fudjoe, 王林林. 氮肥用量及有机无机肥配比对陇中旱农区玉米光合特性及产量的影响. 植物营养与肥料学报, 2020, 26(5): 806-816.
	GUO X J, XIE H J, LI L L, WANG J N, KANG C R, PENG Z K, WANG J B, FUDJOE S, WANG L L. Appropriate nitrogen fertilizer rate and organic N ratio for satisfactory photosynthesis and yield of maize in dry farming area of Longzhong, Gansu Province. Journal of Plant Nutrition and Fertilizers, 2020, 26(5): 806-816. (in Chinese)
[31]	WEI Z B, YING H, GUO X W, ZHUANG M H, CUI Z L, ZHANG F S. Substitution of mineral fertilizer with organic fertilizer in maize systems: A meta-analysis of reduced nitrogen and carbon emissions. Agronomy, 2020, 10(8): 1149-1163.
[32]	VERBRUGGEN E, RÖLING W F M, GAMPER H A, KOWALCHUK G A, VERHOEF H A, VAN DER HEIJDEN M G A. Positive effects of organic farming on below-ground mutualists: Large-scale comparison of mycorrhizal fungal communities in agricultural soils. New Phytologist, 2010, 186(4): 968-979. doi: 10.1111/j.1469-8137.2010.03230.x pmid: 20345633
[33]	BI Y L, QIU L, ZHAKYPBEK Y, JIANG B, CAI Y, SUN H. Combination of plastic film mulching and AMF inoculation promotes maize growth, yield and water use efficiency in the semiarid region of Northwest China. Agricultural Water Management, 2018, 201: 278-286.
[34]	BONFANTE P, GENRE A. Mechanisms underlying beneficial plant-fungus interactions in mycorrhizal symbiosis. Nature Communications, 2010, 1(1): 48-59.
[35]	HODGE A, FITTER A H. Substantial nitrogen acquisition by arbuscular mycorrhizal fungi from organic material has implications for N cycling. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107(31): 13754-13759.
[36]	LIU S W, LIN F, WU S, JI C, SUN Y, JIN Y G, LI S Q, LI Z F, ZOU J W. A meta-analysis of fertilizer-induced soil NO and combined NO+N₂O emissions. Global Change Biology, 2017, 23(6): 2520-2532.
[37]	SENBAYRAM M, CHEN R, BUDAI A, BAKKEN L, DITTERT K. N₂O emission and the N₂O/(N₂O+N₂) product ratio of denitrification as controlled by available carbon substrates and nitrate concentrations. Agriculture, Ecosystems and Environment, 2011, 147(1): 4-12.
[38]	YANG Y D, NIE J W, WANG S, SHI L L, LI Z Z, ZENG Z H, ZANG H D. Differentiated responses of nirS- and nirK-type denitrifiers to 30 years of combined inorganic and organic fertilization in a paddy soil. Archives of Agronomy and Soil Science, 2021, 67(1): 79-92.
[39]	STORER K, COGGAN A, INESON P, HODGE A. Arbuscular mycorrhizal fungi reduce nitrous oxide emissions from N₂O hotspots. New Phytologist, 2018, 220(4): 1285-1295.
[40]	BENDER S F, PLANTENGA F, NEFTEL A, JOCHER M, OBERHOLZER H R, KÖHL L, GILES M, DANIELL T J, VAN DER HEIJDEN M G A. Symbiotic relationships between soil fungi and plants reduce N₂O emissions from soil. The ISME Journal, 2014, 8(6): 1336-1345.
[41]	WU Q S, SRIVASTAVA A K, ZOU Y N. AMF-induced tolerance to drought stress in citrus: A review. Scientia Horticulturae, 2013, 164: 77-87.
[42]	BARRETT G, CAMPBELL C D, FITTER A H, HODGE A. The arbuscular mycorrhizal fungus Glomus hoi can capture and transfer nitrogen from organic patches to its associated host plant at low temperature. Applied Soil Ecology, 2011, 48(1): 102-105.
[43]	VERESOGLOU S D, SEN R, MAMOLOS A P, VERESOGLOU D S. Plant species identity and arbuscular mycorrhizal status modulate potential nitrification rates in nitrogen-limited grassland soils. Journal of Ecology, 2011, 99(6): 1339-1349.
[44]	CHEN Y L, CHEN B D, HU Y J, LI T, ZHANG X, HAO Z P, WANG Y S. Direct and indirect influence of arbuscular mycorrhizal fungi on abundance and community structure of ammonia oxidizing bacteria and archaea in soil microcosms. Pedobiologia, 2013, 56(4/5/6): 205-212.

项目 Item	土壤类型 Soil type	有机肥替代化肥 OF	菌根处理 M	施肥×菌根 OF×M
籽粒产量 Grain yield (g/plant)	砂姜黑土SJ	18.77***	5.85*	0.23
籽粒产量 Grain yield (g/plant)	潮土CT	90.44***	13.99**	0.74
籽粒氮素累积量 Grain N accumulation(mg/plant)	砂姜黑土SJ	30.55***	26.79***	6.24**
籽粒氮素累积量 Grain N accumulation(mg/plant)	潮土CT	40.60***	28.73***	3.17*
N₂O排放通量 N₂O flux rate (μg·m^-2·h^-1)	砂姜黑土SJ	23.74***	46.02***	4.56*
N₂O排放通量 N₂O flux rate (μg·m^-2·h^-1)	潮土CT	8.33**	6.13*	0.51

处理 Treatments		铵态氮 NH₄⁺-N (mg·kg^-1)		硝态氮 NO₃⁻-N (mg·kg^-1)		pH		有机碳 Soil organic carbon (g·kg^-1)		nirK (×10⁶ copies·g^-1)		nirS (×10⁶ copies·g^-1)		nirK/nirS
处理 Treatments		砂姜黑土 SJ	潮土 CT	砂姜黑土 SJ	潮土 CT	砂姜黑土 SJ	潮土 CT	砂姜黑土 SJ	潮土 CT	砂姜黑土 SJ	潮土 CT	砂姜黑土 SJ	潮土 CT	砂姜黑土 SJ	潮土 CT
M-	CK	6.39±0.61de	1.07±0.01	3.91±0.04	3.86±0.05	5.9±0.16	7.8±0.26	12.1±0.2c	8.2±0.1b	5.7±0.5bc	-	55.3±0.9d	-	0.1±0.01b	-
	0%OF	5.72±0.29e	1.08±0.01	3.89±0.03	3.89±0.12	6.0±0.10	7.8±0.28	12.9±0.2b	8.5±0.1ab	6.4±0.6ab	-	78.4±0.6b	-	0.08±0.01bc	-
	25%OF	8.66±0.38a	1.07±0.01	3.98±0.04	3.83±0.04	5.9±0.23	7.8±0.23	12.7±0.3bc	8.4±0.2b	6.0±0.6bc	-	68.1±3.0c	-	0.09±0.01bc	-
	50%OF	8.24±0.32ab	1.05±0.02	3.95±0.04	3.76±0.03	6.0±0.19	7.9±0.24	12.9±0.2b	8.6±0.1ab	7.8±0.7a		100.0±3.5a	-	0.08±0.01bc	-
M+	CK	7.69±0.37abc	1.05±0.01	3.98±0.04	3.71±0.06	6.0±0.23	7.7±0.26	13.1±0.2b	8.5±0.1ab	4.4±0.3c	-	54.8±3.5d	-	0.08±0.01bc	-
	0%OF	7.00±0.26cd	1.07±0.01	3.93±0.09	3.82±0.08	6.2±0.20	7.9±0.12	13.8±0.2a	8.6±0.1ab	4.3±0.4bc	-	37.0±2.4e	-	0.14±0.02a	-
	25%OF	7.23±0.26bcd	1.06±0.01	3.92±0.05	3.69±0.06	6.0±0.05	7.7±0.14	13.8±0.3a	8.6±0.2ab	4.9±0.3bc	-	54.6±3.9d	-	0.09±0.01bc	-
	50%OF	6.58±0.21cde	1.04±0.01	3.91±0.02	3.71±0.11	6.0±0.17	8.2±0.19	13.9±0.3a	8.8±0.1a	5.4±0.8bc	-	81.3±5.1b	-	0.07±0.01c	-
有机肥替代处理OF		15.45***	2.33	0.955	0.95	0.30	0.13	13.58***	3.04*	2.71	-	53.34***	-	5.27**	-
菌根处理 M		5.78*	0.66	0.77	3.95	0.52	2.17	7.89*	5.90*	14.69***	-	67.30***	-	1.16	-
OF×M		0.06	0.06	0.06	0.24	0.03	0.25	1.27	0.17	0.58	-	14.26***	-	6.85**	-

有机肥替代化肥与丛枝菌根真菌互作对砂姜黑土和潮土N₂O排放的影响

The Interactive Effects of Organic Fertilizer Substituting Chemical Fertilizers and Arbuscular Mycorrhizal Fungi on Soil Nitrous Oxide Emission in Shajiang Black Soil and Fluvo-Aquic Soil

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 44

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	牟树佳, 董莉霞, 李广, 燕振刚, 逯玉兰. 基于鲸鱼算法的旱地春小麦农田土壤N₂O排放模型参数优化[J]. 中国农业科学, 2025, 58(3): 537-547.
[2]	李连燚, 王世纪, 姜桂英, 李洋, 杨锦, 朱宣霖, 朱长伟, 王仁卓, 刘芳, 介晓磊, 刘世亮. 轮耕促进豫北潮土区小麦根系生长和产量增加[J]. 中国农业科学, 2024, 57(18): 3626-3641.
[3]	鲁科丹, 路远, 王蕊, 党廷辉. 不同氮素管理模式对黄土旱塬春玉米产量及N₂O排放的影响[J]. 中国农业科学, 2024, 57(18): 3642-3653.
[4]	王宇, 宋一凡, 张荣, 牟海萌, 孙丽芳, 付凯霞, 武紫君, 黄青青, 徐应明, 李鸽子, 王永华, 郭天财. 土壤施用钝化剂与复合微生物肥对冬小麦镉积累的影响[J]. 中国农业科学, 2024, 57(1): 126-141.
[5]	栗晗, 江尚焘, 彭海英, 李培根, 顾长宜, 张金莲, 陈廷速, 徐阳春, 沈其荣, 董彩霞. 接种土著和外源AM真菌对杜梨抗旱性的影响及其适应机制[J]. 中国农业科学, 2024, 57(1): 159-172.
[6]	王箫璇, 张敏, 张鑫尧, 魏鹏, 柴如山, 张朝春, 张亮亮, 罗来超, 郜红建. 不同磷肥对砂姜黑土和红壤磷库转化及冬小麦磷素吸收利用的影响[J]. 中国农业科学, 2023, 56(6): 1113-1126.
[7]	张灵菲, 马垒, 李玉东, 郑福丽, 魏建林, 谭德水, 崔秀敏, 李燕. 有机物料与化肥长期配施对小麦玉米轮作潮土细菌群落和酶活性的影响[J]. 中国农业科学, 2023, 56(19): 3843-3855.
[8]	刘高远, 和爱玲, 杜君, 吕金岭, 聂胜委, 潘秀燕, 许纪东, 李珏, 杨占平. 有机肥替代化肥对砂姜黑土区小麦-玉米轮作系统N₂O排放的影响[J]. 中国农业科学, 2023, 56(16): 3156-3167.
[9]	王树会,陶雯,梁硕,张旭博,孙楠,徐明岗. 长期施用有机肥情景下华北平原旱地土壤固碳及N₂O排放的空间格局[J]. 中国农业科学, 2022, 55(6): 1159-1171.
[10]	李晓立,何堂庆,张晨曦,田明慧,吴梅,李潮海,杨青华,张学林. 等氮量条件下有机肥替代化肥对玉米农田温室气体排放的影响[J]. 中国农业科学, 2022, 55(5): 948-961.
[11]	张学林, 吴梅, 何堂庆, 张晨曦, 田明慧, 李晓立, 侯小畔, 郝晓峰, 杨青华, 李潮海. 秸秆分解对两种类型土壤无机氮和氧化亚氮排放的影响[J]. 中国农业科学, 2022, 55(4): 729-742.
[12]	张英强,张水勤,李燕婷,赵秉强,袁亮. 不同含羧基有机酸改性尿素在石灰性潮土中的转化特征[J]. 中国农业科学, 2022, 55(17): 3355-3364.
[13]	张晨曦, 田明慧, 杨硕, 杜嘉琪, 何堂庆, 仇云鹏, 张学林. 酸性土壤中丛枝菌根真菌菌剂多样性对玉米产量及其磷钾吸收的影响[J]. 中国农业科学, 2022, 55(15): 2899-2910.
[14]	万连杰,何满,李俊杰,田洋,张绩,郑永强,吕强,谢让金,马岩岩,邓烈,易时来. 有机肥替代部分化肥对椪柑生长、品质及土壤特性的影响[J]. 中国农业科学, 2022, 55(15): 2988-3001.
[15]	张学林,何堂庆,张晨曦,田明慧,李晓立,吴梅,周亚男,郝晓峰. 丛枝菌根真菌对玉米生育期土壤N₂O排放的影响[J]. 中国农业科学, 2022, 55(10): 2000-2012.