外源海藻糖提高甘蔗幼苗抗旱能力并促进植株生长

doi:10.3864/j.issn.0578-1752.2023.21.006

中国农业科学 ›› 2023, Vol. 56 ›› Issue (21): 4208-4218.doi: 10.3864/j.issn.0578-1752.2023.21.006

• 耕作栽培·生理生化·农业信息技术 • 上一篇下一篇

外源海藻糖提高甘蔗幼苗抗旱能力并促进植株生长

罗正英¹^,²^,³(), 胡鑫¹^,²(), 刘新龙¹^,², 吴才文¹^,², 吴转娣¹^,², 刘家勇¹^,²(), 曾千春³()

¹ 云南省农业科学院甘蔗研究所/云南甘蔗遗传改良重点实验室/农业农村部甘蔗生物学与遗传育种重点实验室（云南），云南开远 661699
² 热带作物生物育种全国重点实验室，昆明 650221
³ 云南农业大学农学与生物技术学院，昆明 650201

收稿日期:2023-02-01 接受日期:2023-03-17 出版日期:2023-11-01 发布日期:2023-11-06
通信作者:
刘家勇，E-mail： lljjyy1976@163.com。
曾千春，E-mail：zengqianchun@qq.com
联系方式: 罗正英，E-mail：zhengyluo@163.com。胡鑫，E-mail：sugarhuxin@163.com。罗正英和胡鑫为同等贡献作者。
基金资助:
国家重点研发计划(2022YFD2301100); 云南基础研究项目(202201AU070200); 云南省种子种业联合实验室项目(202205AR070001-09); 云南省重大专项计划(202102AE090028); 国家糖料产业技术体系(CARS-170101)

Application of Trehalose Enhances Drought Resistance in Sugarcane Seedlings and Promotes Plant Growth

LUO ZhengYing¹^,²^,³(), HU Xin¹^,²(), LIU XinLong¹^,², WU CaiWen¹^,², WU ZhuanDi¹^,², LIU JiaYong¹^,²(), ZENG QianChun³()

¹ Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Key Laboratory of Sugarcane Genetic Improvement/Key Laboratory of Sugarcane Biology and Genetic Breeding (Yunnan), Ministry of Agriculture and Rural Affairs, Kaiyuan 661699, Yunnan
² National Key Laboratory for Biological Breeding of Tropical Crops, Kunming 650221
³ College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201

Received:2023-02-01 Accepted:2023-03-17 Published:2023-11-01 Online:2023-11-06

摘要/Abstract

摘要：

【目的】研究海藻糖处理能否通过增强甘蔗的防御反应来降低干旱胁迫的不利影响，为干旱条件下甘蔗高产、稳产提供理论依据。【方法】以甘蔗品种ROC22和YZ05-51组培苗为试验材料，以30% PEG6000和12% PEG6000模拟干旱胁迫，设置0、10、100和200 mg·L^-1 4个海藻糖处理浓度。处理9 d后测定组培苗鲜重、干重及不定根数目，明确干旱胁迫下海藻糖促进甘蔗生长的最优浓度。然后，以正常条件为对照，检测在30% PEG6000、12% PEG6000、30% PEG6000+最优浓度海藻糖以及12% PEG6000+最优浓度海藻糖等处理下组培苗丙二醛（MDA）含量、过氧化物酶（POD）和超氧化物歧化酶（SOD）活性。最后，在30% PEG6000和30% PEG6000+最优浓度海藻糖处理后0、12、24和48 h 4个时间点，通过qRT-PCR方法检测YZ05-51组培苗抗旱相关基因ScTPS1、ScSnRK2.3、ScSnRK2.4和ScDREB2b-1的表达水平。【结果】海藻糖处理可缓解30% PEG6000和12% PEG6000干旱胁迫导致的植株鲜重和干重的下降，100 mg·L^-1海藻糖对干旱条件下甘蔗组培苗生长的促进作用优于10和200 mg·L^-1海藻糖处理。30% PEG6000干旱胁迫会导致甘蔗组培苗的MDA含量上升，同时抗氧化酶POD和SOD的活性也发生上调。海藻糖处理则降低了干旱诱导的MDA上升幅度，且进一步增强了POD和SOD的活性，但海藻糖对12% PEG6000胁迫下组培苗的MDA含量和抗氧化酶活性影响不明显。在高浓度PEG6000胁迫下，海藻糖处理会提高甘蔗抗旱基因ScTPS1、ScSnRK2.3、ScSnRK2.4和ScDREB2b-1的表达水平。【结论】海藻糖处理可缓解干旱对甘蔗组培苗的生长抑制，促进不定根生长。干旱胁迫下，海藻糖通过提高抗氧化酶POD和SOD的活性，降低干旱胁迫引起的氧化毒性；同时，海藻糖处理诱导抗旱基因ScTPS1、ScSnRK2.3、ScSnRK2.4和ScDREB2b-1的表达，表明施加海藻糖可提高甘蔗的抗旱能力。研究结果可为甘蔗抗旱育种和生产实践中制定甘蔗抗旱策略提供参考。

关键词: 甘蔗, 海藻糖, 抗旱性, 抗氧化酶, 基因表达

Abstract:

【Objective】This study aims to investigate whether application of trehalose could mitigate the adverse effects of drought stress by enhancing the defense response in sugarcane (Saccharum spp. hybrid), and to provide a theoretical basis for stable high yield of sugarcane under drought conditions.【Method】The present investigation was conducted to assess ameliorative effects of adding trehalose on the growth, malondialdehyde (MDA) content, and antioxidant responses of two sugarcane cultivars ROC22 and YZ05-51 under the treatment of 30% PEG6000 and 12% PEG6000. In the preliminary experiment, four treatment concentrations of trehalose were set at 0, 10, 100 and 200 mg·L^-1. The dry weight, fresh weight and adventitious roots of tissue-cultured sugarcane seedlings were measured at 9 d after treatment to clarify the optimal concentration of trehalose in promoting sugarcane growth under drought stress. Then, the MDA content and the activity of antioxidant enzyme peroxidase (POD) and superoxide dismutase (SOD) were determined under control, drought stress and drought stress combined with optimal trehalose treatment. Finally, the expression level of drought-resistance genes ScTPS1, ScSnRK2.3, ScSnRK2.4 and ScDREB2b-1 was quantified by qRT-PCR at 0, 12, 24 and 48 h in YZ05-51 after exposed to 30% PEG6000 and 30% PEG6000 adding with optimal trehalose concentration, respectively.【Result】Application of trehalose could alleviate the drought-induced decrease of fresh weight and dry weight of two sugarcane cultivars, and the growth recovery of plantlet was better in 100 mg·L^-1 trehalose group than that in 10 and 200 mg·L^-1 trehalose groups. Under 30% PEG6000 stress, the MDA content was notably increased, and a considerable improvement was recorded in the activity of the antioxidant enzymes POD and SOD. Adding trehalose significantly reduced the content of drought-induced MDA, and enhanced the activity of POD and SOD, respectively. However, external trehalose had little effect on the MDA content and antioxidant enzyme activity of tissue-cultured sugarcane seedlings under 12% PEG6000 stress. Compared with drought controls, the expression of drought-resistance genes ScTPS1, ScSnRK2.3, ScSnRK2.4 and ScDREB2b-1 was up-regulated in the trehalose-treated plantlet.【Conclusion】Application of trehalose can alleviate the negative impact of drought on the growth of sugarcane seedlings, and promote adventitious root growth. External addition of trehalose may reduce the oxidative toxicity caused by drought stress by increasing the activity of antioxidant enzymes POD and SOD. Meanwhile, application of trehalose induces the expression of drought-resistance genes ScTPS1, ScSnRK2.3, ScSnRK2.4, and ScDREB2b-1, indicating that applying trehalose can improve the drought resistance of sugarcane. The results will provide a reference for developing drought resistance strategies in sugarcane breeding and productive practice.

Key words: sugarcane, trehalose, drought resistance, antioxidant enzyme, gene expression

罗正英, 胡鑫, 刘新龙, 吴才文, 吴转娣, 刘家勇, 曾千春. 外源海藻糖提高甘蔗幼苗抗旱能力并促进植株生长[J]. 中国农业科学, 2023, 56(21): 4208-4218.

LUO ZhengYing, HU Xin, LIU XinLong, WU CaiWen, WU ZhuanDi, LIU JiaYong, ZENG QianChun. Application of Trehalose Enhances Drought Resistance in Sugarcane Seedlings and Promotes Plant Growth[J]. Scientia Agricultura Sinica, 2023, 56(21): 4208-4218.

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[44]	BOUDSOCQ M, BARBIER-BRYGOO H, LAURIERE C. Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana. The Journal of Biological Chemistry, 2004, 279(40): 41758-41766. doi: 10.1074/jbc.M405259200
[45]	KOBAYASHI Y, YAMAMOTO S, MINAMI H, KAGAYA Y, HATTORI T. Differential activation of the rice sucrose nonfermenting1- related protein kinase 2 family by hyperosmotic stress and abscisic acid. The Plant Cell, 2004, 16(5): 1163-1177. doi: 10.1105/tpc.019943
[46]	谭秦亮, 李长宁, 杨丽涛, 李杨瑞. 甘蔗ABA信号转导关键酶SoSnRK2.1基因的克隆与表达分析. 作物学报, 2013, 39(12): 2162-2170. doi: 10.3724/SP.J.1006.2013.02162
	TAN Q L, LI C N, YANG L T, LI Y R. Cloning and expression analysis of abscisic acid signal transduction key enzyme gene SoSnRK2.1 from sugarcane. Acta Agronomica Sinica, 2013, 39(12): 2162-2170. (in Chinese) doi: 10.3724/SP.J.1006.2013.02162

基因编号 Gene ID	正向引物序列 Forward primer sequence (5°-3°)	反向引物序列 Reverse primer sequence (5°-3°)
GAPDH	CACGGCCACTGGAAGCA	TCCTCAGGGTTCCTGATGCC
ScTPS1	TGTGCCAACAAGAACTGACG	GCTCACAAGGTTCATCCCATC
ScSnRK2.3	ACCACAGCAGGGCGATTC	ATGCAGCAAACCAGACTTTGAGTA
ScSnRK2.4	ACCTACCACGGGAACTCA	TCATCCGAATACTCACTGCT
ScDREB2b-1	ACACAATGTGGGTACCGTGG	ATAGGCCCTAGCTGCATCCT

外源海藻糖提高甘蔗幼苗抗旱能力并促进植株生长

Application of Trehalose Enhances Drought Resistance in Sugarcane Seedlings and Promotes Plant Growth

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