外源海藻糖提高甘蔗幼苗抗旱能力并促进植株生长

doi:10.3864/j.issn.0578-1752.2023.21.006

中国农业科学 ›› 2023, Vol. 56 ›› Issue (21): 4208-4218.doi: 10.3864/j.issn.0578-1752.2023.21.006

• 耕作栽培·生理生化·农业信息技术 • 上一篇下一篇

外源海藻糖提高甘蔗幼苗抗旱能力并促进植株生长

罗正英¹^,²^,³(), 胡鑫¹^,²(), 刘新龙¹^,², 吴才文¹^,², 吴转娣¹^,², 刘家勇¹^,²(), 曾千春³()

¹ 云南省农业科学院甘蔗研究所/云南甘蔗遗传改良重点实验室/农业农村部甘蔗生物学与遗传育种重点实验室（云南），云南开远 661699
² 热带作物生物育种全国重点实验室，昆明 650221
³ 云南农业大学农学与生物技术学院，昆明 650201

收稿日期:2023-02-01 接受日期:2023-03-17 出版日期:2023-11-01 发布日期:2023-11-06
通信作者:
刘家勇，E-mail： lljjyy1976@163.com。
曾千春，E-mail：zengqianchun@qq.com
联系方式: 罗正英，E-mail：zhengyluo@163.com。胡鑫，E-mail：sugarhuxin@163.com。罗正英和胡鑫为同等贡献作者。
基金资助:
国家重点研发计划(2022YFD2301100); 云南基础研究项目(202201AU070200); 云南省种子种业联合实验室项目(202205AR070001-09); 云南省重大专项计划(202102AE090028); 国家糖料产业技术体系(CARS-170101)

Application of Trehalose Enhances Drought Resistance in Sugarcane Seedlings and Promotes Plant Growth

LUO ZhengYing¹^,²^,³(), HU Xin¹^,²(), LIU XinLong¹^,², WU CaiWen¹^,², WU ZhuanDi¹^,², LIU JiaYong¹^,²(), ZENG QianChun³()

¹ Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Key Laboratory of Sugarcane Genetic Improvement/Key Laboratory of Sugarcane Biology and Genetic Breeding (Yunnan), Ministry of Agriculture and Rural Affairs, Kaiyuan 661699, Yunnan
² National Key Laboratory for Biological Breeding of Tropical Crops, Kunming 650221
³ College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201

Received:2023-02-01 Accepted:2023-03-17 Published:2023-11-01 Online:2023-11-06

摘要/Abstract

摘要：

【目的】研究海藻糖处理能否通过增强甘蔗的防御反应来降低干旱胁迫的不利影响，为干旱条件下甘蔗高产、稳产提供理论依据。【方法】以甘蔗品种ROC22和YZ05-51组培苗为试验材料，以30% PEG6000和12% PEG6000模拟干旱胁迫，设置0、10、100和200 mg·L^-1 4个海藻糖处理浓度。处理9 d后测定组培苗鲜重、干重及不定根数目，明确干旱胁迫下海藻糖促进甘蔗生长的最优浓度。然后，以正常条件为对照，检测在30% PEG6000、12% PEG6000、30% PEG6000+最优浓度海藻糖以及12% PEG6000+最优浓度海藻糖等处理下组培苗丙二醛（MDA）含量、过氧化物酶（POD）和超氧化物歧化酶（SOD）活性。最后，在30% PEG6000和30% PEG6000+最优浓度海藻糖处理后0、12、24和48 h 4个时间点，通过qRT-PCR方法检测YZ05-51组培苗抗旱相关基因ScTPS1、ScSnRK2.3、ScSnRK2.4和ScDREB2b-1的表达水平。【结果】海藻糖处理可缓解30% PEG6000和12% PEG6000干旱胁迫导致的植株鲜重和干重的下降，100 mg·L^-1海藻糖对干旱条件下甘蔗组培苗生长的促进作用优于10和200 mg·L^-1海藻糖处理。30% PEG6000干旱胁迫会导致甘蔗组培苗的MDA含量上升，同时抗氧化酶POD和SOD的活性也发生上调。海藻糖处理则降低了干旱诱导的MDA上升幅度，且进一步增强了POD和SOD的活性，但海藻糖对12% PEG6000胁迫下组培苗的MDA含量和抗氧化酶活性影响不明显。在高浓度PEG6000胁迫下，海藻糖处理会提高甘蔗抗旱基因ScTPS1、ScSnRK2.3、ScSnRK2.4和ScDREB2b-1的表达水平。【结论】海藻糖处理可缓解干旱对甘蔗组培苗的生长抑制，促进不定根生长。干旱胁迫下，海藻糖通过提高抗氧化酶POD和SOD的活性，降低干旱胁迫引起的氧化毒性；同时，海藻糖处理诱导抗旱基因ScTPS1、ScSnRK2.3、ScSnRK2.4和ScDREB2b-1的表达，表明施加海藻糖可提高甘蔗的抗旱能力。研究结果可为甘蔗抗旱育种和生产实践中制定甘蔗抗旱策略提供参考。

关键词: 甘蔗, 海藻糖, 抗旱性, 抗氧化酶, 基因表达

Abstract:

【Objective】This study aims to investigate whether application of trehalose could mitigate the adverse effects of drought stress by enhancing the defense response in sugarcane (Saccharum spp. hybrid), and to provide a theoretical basis for stable high yield of sugarcane under drought conditions.【Method】The present investigation was conducted to assess ameliorative effects of adding trehalose on the growth, malondialdehyde (MDA) content, and antioxidant responses of two sugarcane cultivars ROC22 and YZ05-51 under the treatment of 30% PEG6000 and 12% PEG6000. In the preliminary experiment, four treatment concentrations of trehalose were set at 0, 10, 100 and 200 mg·L^-1. The dry weight, fresh weight and adventitious roots of tissue-cultured sugarcane seedlings were measured at 9 d after treatment to clarify the optimal concentration of trehalose in promoting sugarcane growth under drought stress. Then, the MDA content and the activity of antioxidant enzyme peroxidase (POD) and superoxide dismutase (SOD) were determined under control, drought stress and drought stress combined with optimal trehalose treatment. Finally, the expression level of drought-resistance genes ScTPS1, ScSnRK2.3, ScSnRK2.4 and ScDREB2b-1 was quantified by qRT-PCR at 0, 12, 24 and 48 h in YZ05-51 after exposed to 30% PEG6000 and 30% PEG6000 adding with optimal trehalose concentration, respectively.【Result】Application of trehalose could alleviate the drought-induced decrease of fresh weight and dry weight of two sugarcane cultivars, and the growth recovery of plantlet was better in 100 mg·L^-1 trehalose group than that in 10 and 200 mg·L^-1 trehalose groups. Under 30% PEG6000 stress, the MDA content was notably increased, and a considerable improvement was recorded in the activity of the antioxidant enzymes POD and SOD. Adding trehalose significantly reduced the content of drought-induced MDA, and enhanced the activity of POD and SOD, respectively. However, external trehalose had little effect on the MDA content and antioxidant enzyme activity of tissue-cultured sugarcane seedlings under 12% PEG6000 stress. Compared with drought controls, the expression of drought-resistance genes ScTPS1, ScSnRK2.3, ScSnRK2.4 and ScDREB2b-1 was up-regulated in the trehalose-treated plantlet.【Conclusion】Application of trehalose can alleviate the negative impact of drought on the growth of sugarcane seedlings, and promote adventitious root growth. External addition of trehalose may reduce the oxidative toxicity caused by drought stress by increasing the activity of antioxidant enzymes POD and SOD. Meanwhile, application of trehalose induces the expression of drought-resistance genes ScTPS1, ScSnRK2.3, ScSnRK2.4, and ScDREB2b-1, indicating that applying trehalose can improve the drought resistance of sugarcane. The results will provide a reference for developing drought resistance strategies in sugarcane breeding and productive practice.

Key words: sugarcane, trehalose, drought resistance, antioxidant enzyme, gene expression

罗正英, 胡鑫, 刘新龙, 吴才文, 吴转娣, 刘家勇, 曾千春. 外源海藻糖提高甘蔗幼苗抗旱能力并促进植株生长[J]. 中国农业科学, 2023, 56(21): 4208-4218.

LUO ZhengYing, HU Xin, LIU XinLong, WU CaiWen, WU ZhuanDi, LIU JiaYong, ZENG QianChun. Application of Trehalose Enhances Drought Resistance in Sugarcane Seedlings and Promotes Plant Growth[J]. Scientia Agricultura Sinica, 2023, 56(21): 4208-4218.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2023.21.006

https://www.chinaagrisci.com/CN/Y2023/V56/I21/4208

图/表 6

表1

图1

图2

图3

图4

图5

参考文献 46

[1]	TAI P Y P, MILLER J D. Germplasm diversity among four sugarcane species for sugar composition. Crop Science, 2002, 42(3): 958-964. doi: 10.2135/cropsci2002.9580
[2]	刘三梅, 杨清辉, 李秀年, 黄桂萍, 肖关丽. 不同生育时期干旱胁迫对甘蔗形态指标及生理特性的影响. 南方农业学报, 2016, 47(8): 1273-1278.
	LIU S M, YANG Q H, LI X N, HUANG G P, XIAO G L. Effects of drought stress on morphological index and physiological characteristics of sugarcane at different growth stages. Journal of Southern Agriculture, 2016, 47(8): 1273-1278. (in Chinese)
[3]	DEVI K, GOMATHI R, ARUN KUMAR R, MANIMEKALAI R, SELVI A. Field tolerance and recovery potential of sugarcane varieties subjected to drought. Indian Journal of Plant Physiology, 2018, 23(2): 271-282. doi: 10.1007/s40502-018-0367-7
[4]	DINH H T, WATANABLE K, TAKARAGAWA H, KAWAMITSU Y. Effects of drought stress at early growth stage on response of sugarcane to different nitrogen application. Sugar Tech, 2018, 20(4): 420-430. doi: 10.1007/s12355-017-0566-y
[5]	李海碧, 桂意云, 张荣华, 韦金菊, 杨荣仲, 张小秋, 李杨瑞, 周会, 刘昔辉. 甘蔗抗旱性及抗旱育种研究进展. 分子植物育种, 2019, 17(10): 3406-3415.
	LI H B, GUI Y Y, ZHANG R H, WEI J J, YANG R Z, ZHANG X Q, LI Y R, ZHOU H, LIU X H. Research progress on drought resistance and drought-resistant breeding of sugarcane. Molecular Plant Breeding, 2019, 17(10): 3406-3415. (in Chinese)
[6]	陆珍, 杨丽涛, 邢永秀, 李杨瑞. 甘蔗响应干旱胁迫研究进展. 分子植物育种, 2023, 21(6): 2051-2060.
	LU Z, YANG L T, XING Y X, LI Y R. Research advances on sugarcane response to drought stress. Molecular Plant Breeding, 2023, 21(6): 2051-2060. (in Chinese)
[7]	HASSAN M U, NAWAZ M, SHAH A N, RAZA A, BARBANTI L, SKALICKY M, HASHEM M, BRESTIC M, PANDEY S, ALAMRI S, MOSTAFA Y, SABAGH A E L, QARI S H. Trehalose: A key player in plant growth regulation and tolerance to abiotic stresses. Journal of Plant Growth Regulation, 2023, 42(8): 4935-4957. doi: 10.1007/s00344-022-10851-7
[8]	SHAO J, WU W, RASUL F, MUNIR H, HUANG K, AWAN M I, ALBISHI T S, ARSHAD M, HU Q, HUANG G, HASSAN M U, AAMER M, QARI S H. Trehalose induced drought tolerance in plants: Physiological and molecular responses. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2022, 50(1): 12584. doi: 10.15835/nbha50112584
[9]	KOSAR F, AKRAM N A, ASHRAF M, AHMAD A, ALYEMENI M N, AHMAD P. Impact of exogenously applied trehalose on leaf biochemistry, achene yield and oil composition of sunflower under drought stress. Physiologia Plantarum, 2021, 172(2): 317-333. doi: 10.1111/ppl.v172.2
[10]	LIN Q, WANG S, DAO Y, WANG J, WANG K. Arabidopsis thaliana trehalose-6-phosphate phosphatase gene TPPI enhances drought tolerance by regulating stomatal apertures. Journal of Experimental Botany, 2020, 71(14): 4285-4297. doi: 10.1093/jxb/eraa173
[11]	ALAM M M, NAHAR K, HASANUZZAMAN M, FUJITA M. Trehalose-induced drought stress tolerance: A comparative study among different Brassica species. Plant Omics, 2014, 7(4): 271-283.
[12]	叶玉秀, 陆大雷, 王飞兵, 陈新红, 戚名扬, 何润, 陆卫平. 干旱胁迫下外源海藻糖对糯玉米幼苗生理特性的影响. 玉米科学, 2020, 28(3): 80-86.
	YE Y X, LU D L, WANG F B, CHEN X H, QI M Y, HE R, LU W P. Effects of exogenous trehalose on physiological characteristics in waxy maize seedlings under drought stress. Journal of Maize Sciences, 2020, 28(3): 80-86. (in Chinese)
[13]	李佳馨, 李霞, 谢寅峰. 外源海藻糖增强高表达转玉米C₄型PEPC水稻耐旱性的机制. 植物学报, 2021, 56(3): 296-314.
	LI J X, LI X, XIE Y F. Mechanism on drought tolerance enhanced by exogenous trehalose in C₄-PEPC rice. Chinese Bulletin of Botany, 2021, 56(3): 296-314. (in Chinese)
[14]	徐向丽, 易克, 蒋红梅, 李永平, 马文广, 郑昀晔, 姚恒. 外源海藻糖对干旱胁迫下烟草幼苗抗旱性的影响. 安徽农业科学, 2010, 38(33): 18675-18677.
	XU X L, YI K, JIANG H M, LI Y P, MA W G, ZHENG Y Y, YAO H. Effect of exogenous trehalose on the drought-resisting of tobacco seedling under drought stress. Journal of Anhui Agricultural Sciences, 2010, 38(33): 18675-18677. (in Chinese)
[15]	MAHMOUD R A, HASSAN O S, ABOU-HASHISH A, AMIN A. Role of trehalose during recovery from drought stress in micropropagated banana (Musa spp.) transplants. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2017, 8(2): 1335-1345.
[16]	韩俊艳, 何丹, 邹春静, 罗音. 海藻糖响应植物非生物胁迫反应机制研究进展. 生物学教学, 2021, 46(10): 2-4.
	HAN J Y, HE D, ZOU C J, LUO Y. Research progress on the mechanism of trehalose in response to plant abiotic stress. Biology Teaching, 2021, 46(10): 2-4. (in Chinese)
[17]	ZULFIQAR F, CHEN J J, FINNEGAN P M, YOUNIS A, NAFEES M, ZORRIG W, HAMED K B. Application of trehalose and salicylic acid mitigates drought stress in sweet basil and improves plant growth. Plants, 2021, 10(6): 1078. doi: 10.3390/plants10061078
[18]	ZULFIQAR F, CHEN J, FINNEGAN P M, NAFEES M, YOUNIS A, SHAUKAT N, LATIF N, ABIDEEN Z, ZAID A, RAZA A, SIDDIQUI Z S, HAMED K B. Foliar application of trehalose or 5-aminolevulinic acid improves photosynthesis and biomass production in drought stressed Alpinia zerumbet. Agriculture, 2021, 11(10): 908. doi: 10.3390/agriculture11100908
[19]	KOSAR F, AKRAM N A, SADIQ M, AL-QURAINY F, ASHRAF M. Trehalose: A key organic osmolyte effectively involved in plant abiotic stress tolerance. Journal of Plant Growth Regulation, 2019, 38(2): 606-618. doi: 10.1007/s00344-018-9876-x
[20]	JUNIOR N N, NICOLAU M S, MANTOVANINI L J, ZINGARETTI S M. Expression analysis of two genes coding for trehalose-6- phosphate synthase (TPS), in sugarcane (Saccharum spp.) under water stress. American Journal of Plant Sciences, 2013, 4(12): 91-99. doi: 10.4236/ajps.2013.412A3011
[21]	HU X, WU Z D, LUO Z Y, BURNER D M, PAN Y B, WU C W. Genome-wide analysis of the trehalose-6-phosphate synthase (TPS) gene family and expression profiling of ScTPS genes in sugarcane. Agronomy, 2020, 10(7): 969. doi: 10.3390/agronomy10070969
[22]	YU W, ZHAO R, WANG L, ZHANG S, LI R, SHENG J, SHEN L. ABA signaling rather than ABA metabolism is involved in trehalose-induced drought tolerance in tomato plants. Planta, 2019, 250(2): 643-655. doi: 10.1007/s00425-019-03195-2 pmid: 31144110
[23]	MACINTYRE A M, MELINE V, GORMAN Z, AUGUSTINE S P, DYE C J, HAMILTON C D, IYER-PASCUZZI A S, KOLOMIETS M V, MCCULLOH K A, ALLEN C. Trehalose increases tomato drought tolerance, induces defenses, and increases resistance to bacterial wilt disease. PLoS ONE, 2022, 17(4): e0266254.
[24]	PHAN T T, SUN B, NIU J Q, TAN Q L, LI J, YANG L T, LI Y R. Overexpression of sugarcane gene SoSnRK2.1 confers drought tolerance in transgenic tobacco. Plant Cell Reports, 2016, 35(9): 1891-1905. doi: 10.1007/s00299-016-2004-0
[25]	CHEN Y, LI Z, SUN T, WANG D, WANG Z, ZHANG C, QUE Y, GUO J, XU L, SU Y. Sugarcane ScDREB2B-1 confers drought stress tolerance in transgenic Nicotiana benthamiana by regulating the ABA signal, ROS level and stress-related gene expression. International Journal of Molecular Sciences, 2022, 23(17): 9557. doi: 10.3390/ijms23179557
[26]	刘家勇, 赵培方, 杨昆, 夏红明, 吴才文, 陈学宽, 赵俊, 杨洪昌, 昝逢刚, 姚丽, 吴转娣, 赵丽萍, 覃伟. 甘蔗新品种云蔗05-51的选育. 中国糖料, 2016, 38(1): 8-10.
	LIU J Y, ZHAO P F, YANG K, XIA H M, WU C W, CHEN X K, ZHAO J, YANG H C, ZAN F G, YAO L, WU Z D, ZHAO L P, QIN W. Breeding of new sugarcane variety, Yunzhe05-51. Sugar Crops of China, 2016, 38(1): 8-10. (in Chinese)
[27]	王志恒, 赵延蓉, 黄思麒, 王悦娟, 起金娥, 魏玉清. 外源海藻糖影响甜高粱幼苗抗旱性的生理生化机制. 植物生理学报, 2022, 58(4): 654-666.
	WANG Z H, ZHAO Y R, HUANG S Q, WANG Y J, QI J E, WEI Y Q. Physiological and biochemical mechanism of exogenous trehalose on drought resistance in sweet sorghum seedlings. Plant Physiology Journal, 2022, 58(4): 654-666. (in Chinese)
[28]	ALDESUQUY H S, GHANEM H E. Exogenous salicylic acid and trehalose ameliorate short term drought stress in wheat cultivars by up-regulating membrane characteristics and antioxidant defense system. Journal of Horticulture, 2015, 2(2): 1000139.
[29]	EISA G S A, IBRAHIM S A. Mitigation the harmful effects of diluted sea water salinity on physiological and anatomical traits by foliar spray with trehalose and sodium nitroprusside of cowpea plants. Middle East Journal of Agriculture, 2016, 5(4): 672-686.
[30]	REDILLAS M C, PARK S H, LEE J W, KIM Y S, JEONG J S, JUNG H, BANG S W, HAHN T R, KIM J K. Accumulation of trehalose increases soluble sugar contents in rice plants conferring tolerance to drought and salt stress. Plant Biotechnology Reports, 2012, 6: 89-96. doi: 10.1007/s11816-011-0210-3
[31]	REZVANI S, SHARIATI S. Analysis of trehalose in Arabidopsis thaliana L. in addition, helpful at all stages of HD. Rasayan Journal of Chemistry, 2009, 2: 267-270.
[32]	FICHTNER F, LUNN J E. The role of trehalose 6-phosphate (Tre6P) in plant metabolism and development. Annual Review of Plant Biology, 2021, 72: 737-760. doi: 10.1146/annurev-arplant-050718-095929 pmid: 33428475
[33]	吴凯朝, 黄诚梅, 邓智年, 曹辉庆, 魏源文, 徐林, 李杨瑞, 杨丽涛. 干旱后复水对甘蔗伸长期生理生化特性的影响. 南方农业学报, 2015, 46(7): 1166-1172.
	WU K C, HUANG C M, DENG Z N, CAO H Q, WEI Y W, XU L, LI Y R, YANG L T. Effects of drought stress and re-watering on physiological-biochemical characteristics in sugarcane at elongation stage. Journal of Southern Agriculture, 2015, 46(7): 1166-1172. (in Chinese)
[34]	黄有总, 徐建云, 陈超君, 何雪银. 几个甘蔗新品种的抗旱性和抗寒性比较研究. 广西农业生物科学, 2002, 21(2): 101-104.
	HUANG Y Z, XU J Y, CHEN C J, HE X Y. Comparative tests on drought resistance and frost resistance of several new sugarcane varieties. Journal of Guangxi Agricultural and Biological Science, 2002, 21(2): 101-104. (in Chinese)
[35]	刘硕, 樊仙, 李如丹, 邓军, 刀静梅, 全怡吉, 杨绍林, 张跃彬. 4个甘蔗主栽品种对干旱胁迫的生理响应. 热带作物学报, 2022, 43(9): 1812-1823.
	LIU S, FAN X, LI R D, DENG J, DAO J M, QUAN Y J, YANG S L, ZHANG Y B. Physiological responses of four major sugarcane cultivars to drought stress. Chinese Journal of Tropical Crops, 2022, 43(9): 1812-1823. (in Chinese)
[36]	谭秦亮, 程琴, 潘成列, 朱鹏锦, 李佳慧, 宋奇琦, 农泽梅, 周全光, 庞新华, 吕平. 干旱胁迫对甘蔗新品种桂热2号生理指标的影响. 作物杂志, 2022(3): 161-167.
	TAN Q L, CHENG Q, PAN C L, ZHU P J, LI J H, SONG Q Q, NONG Z M, ZHOU Q G, PANG X H, LÜ P. Effects of drought stress on physiological indexes of new sugarcane variety Guire 2. Crops, 2022(3): 161-167. (in Chinese)
[37]	SHAFIQ S, AKRAM N A, ASHRAF M. Does exogenously-applied trehalose alter oxidative defense system in the edible part of radish (Raphanus sativus L.) under water-deficit conditions?. Scientia Horticulturae, 2015, 185: 68-75. doi: 10.1016/j.scienta.2015.01.010
[38]	SADAK M S, EL-BASSIOUNY H M S, DAWOOD M G. Role of trehalose on antioxidant defense system and some osmolytes of quinoa plants under water deficit. Bulletin of the National Research Centre, 2019, 43: 5. doi: 10.1186/s42269-018-0039-9
[39]	GOLLDACK D, LUKING L, YANG O. Plant tolerance to drought and salinity: Stress regulating transcription factors and their functional significance in the cellular transcriptional network. Plant Cell Reports, 2011, 30(8): 1383-1391. doi: 10.1007/s00299-011-1068-0 pmid: 21476089
[40]	LI Z, WEI X, TONG X, ZHAO J, LIU X, WANG H, TANG L, SHU Y, LI G, WANG Y, YING J, JIAO G, HU H, HU P, ZHANG J. The OsNAC23-Tre6P-SnRK1a feed-forward loop regulates sugar homeostasis and grain yield in rice. Molecular Plant, 2022, 15: 706-722. doi: 10.1016/j.molp.2022.01.016
[41]	GRIFFITHS C A, SAGAR R, GENG Y, PRIMAVESI L F, PATEL M K, PASSARELLI M K, GILMORE I S, STEVEN R T, BUNCH J, PAUL M J, DAVIS B G. Chemical intervention in plant sugar signalling increases yield and resilience. Nature, 2016, 540(7634): 574-578. doi: 10.1038/nature20591
[42]	LI H W, ZANG B S, DENG X W, WANG X P. Overexpression of the trehalose-6-phosphate synthase gene OsTPS1 enhances abiotic stress tolerance in rice. Planta, 2011, 234(5): 1007-1018. doi: 10.1007/s00425-011-1458-0
[43]	FENG Y, CHEN X, HE Y, KOU X, XUE Z. Effects of exogenous trehalose on the metabolism of sugar and abscisic acid in tomato seedlings under salt stress. Transactions of Tianjin University, 2019, 25(5): 451-471. doi: 10.1007/s12209-019-00214-x
[44]	BOUDSOCQ M, BARBIER-BRYGOO H, LAURIERE C. Identification of nine sucrose nonfermenting 1-related protein kinases 2 activated by hyperosmotic and saline stresses in Arabidopsis thaliana. The Journal of Biological Chemistry, 2004, 279(40): 41758-41766. doi: 10.1074/jbc.M405259200
[45]	KOBAYASHI Y, YAMAMOTO S, MINAMI H, KAGAYA Y, HATTORI T. Differential activation of the rice sucrose nonfermenting1- related protein kinase 2 family by hyperosmotic stress and abscisic acid. The Plant Cell, 2004, 16(5): 1163-1177. doi: 10.1105/tpc.019943
[46]	谭秦亮, 李长宁, 杨丽涛, 李杨瑞. 甘蔗ABA信号转导关键酶SoSnRK2.1基因的克隆与表达分析. 作物学报, 2013, 39(12): 2162-2170. doi: 10.3724/SP.J.1006.2013.02162
	TAN Q L, LI C N, YANG L T, LI Y R. Cloning and expression analysis of abscisic acid signal transduction key enzyme gene SoSnRK2.1 from sugarcane. Acta Agronomica Sinica, 2013, 39(12): 2162-2170. (in Chinese) doi: 10.3724/SP.J.1006.2013.02162

基因编号 Gene ID	正向引物序列 Forward primer sequence (5°-3°)	反向引物序列 Reverse primer sequence (5°-3°)
GAPDH	CACGGCCACTGGAAGCA	TCCTCAGGGTTCCTGATGCC
ScTPS1	TGTGCCAACAAGAACTGACG	GCTCACAAGGTTCATCCCATC
ScSnRK2.3	ACCACAGCAGGGCGATTC	ATGCAGCAAACCAGACTTTGAGTA
ScSnRK2.4	ACCTACCACGGGAACTCA	TCATCCGAATACTCACTGCT
ScDREB2b-1	ACACAATGTGGGTACCGTGG	ATAGGCCCTAGCTGCATCCT

外源海藻糖提高甘蔗幼苗抗旱能力并促进植株生长

Application of Trehalose Enhances Drought Resistance in Sugarcane Seedlings and Promotes Plant Growth

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 46

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	渠清, 刘宁, 邹金鹏, 张雅璇, 贾慧, 孙蔓莉, 曹志艳, 董金皋. 拟轮枝镰孢与玉米籽粒互作的差异基因筛选及代谢通路分析[J]. 中国农业科学, 2023, 56(6): 1086-1101.
[2]	邹婷, 刘丽莉, 向建华, 周定港, 吴金锋, 李莓, 李宝, 张大为, 严明理. 芸薹属植物MYBL2基因的克隆及其在A、B、C基因组中的PCR鉴别[J]. 中国农业科学, 2023, 56(3): 416-429.
[3]	李美璇, 张向昆, 王莉, 乔月莲, 师校欣, 杜国强. 沙地葡萄茎痘相关病毒在‘阳光玫瑰’葡萄树不同物候期和不同部位的变化规律[J]. 中国农业科学, 2023, 56(21): 4234-4244.
[4]	杨丽, 曹洪波, 张学英, 翟含含, 李辛淼, 彭佳伟, 田义, 陈海江. 桃生长势相关基因PpSAUR73功能鉴定[J]. 中国农业科学, 2023, 56(20): 4072-4086.
[5]	许福春, 赵静若, 张振楠, 胡改元, 龙璐. 陆地棉GhCPR5的克隆及其在抗病中的功能分析[J]. 中国农业科学, 2023, 56(19): 3747-3758.
[6]	李燕, 陶柯宇, 胡悦, 李永祥, 张登峰, 李春辉, 何冠华, 宋燕春, 石云素, 黎裕, 王天宇, 邹华文, 刘旭洋. 玉米ZCN7在调控花期抗旱性中的作用[J]. 中国农业科学, 2023, 56(16): 3051-3061.
[7]	刘自刚, 魏家萍, 崔俊美, 武泽峰, 方彦, 董小云, 郑国强. 我国冬油菜北移的现状、问题与对策[J]. 中国农业科学, 2023, 56(15): 2854-2862.
[8]	张克坤,陈可钦,李婉平,乔浩蓉,张俊霞,刘凤之,房玉林,王海波. 灌水量对限根栽培‘阳光玫瑰’葡萄果实发育与香气物质积累的影响[J]. 中国农业科学, 2023, 56(1): 129-143.
[9]	古丽旦,刘洋,李方向,成卫宁. 小麦吸浆虫小热激蛋白基因Hsp21.9的克隆及在滞育过程与温度胁迫下的表达特性[J]. 中国农业科学, 2023, 56(1): 79-89.
[10]	王思彤,陈艳,罗雨嘉,杨缘缘,蒋志洋,蒋鑫怡,钟樊,陈好,徐红星,吴俨,段红霞,唐斌. 三种新型化合物对草地贪夜蛾海藻糖与几丁质代谢及生长发育的影响[J]. 中国农业科学, 2022, 55(8): 1568-1578.
[11]	束婧婷,单艳菊,姬改革,章明,屠云洁,刘一帆,巨晓军,盛中伟,唐燕飞,李华,邹剑敏. 广西麻鸡m6A甲基转移酶基因表达与肌纤维类型及成肌分化的关系[J]. 中国农业科学, 2022, 55(3): 589-601.
[12]	郭绍雷, 许建兰, 王晓俊, 宿子文, 张斌斌, 马瑞娟, 俞明亮. 桃XTH家族基因鉴定及其在桃果实贮藏过程中的表达特性[J]. 中国农业科学, 2022, 55(23): 4702-4716.
[13]	郝艳,李晓颍,叶茂,刘亚婷,王天宇,王海静,张立彬,肖啸,武军凯. ‘21世纪’桃与‘久脆’桃及其杂交后代果实挥发性成分特征分析[J]. 中国农业科学, 2022, 55(22): 4487-4499.
[14]	杜金霞,李奕莎,李美霖,陈文浛,张木清. 甘蔗不同基因型对白条病抗性的评价[J]. 中国农业科学, 2022, 55(21): 4118-4130.
[15]	康忱,赵雪芳,李亚栋,田哲娟,王鹏,吴志明. 黄瓜CC-NBS-LRR家族基因鉴定及在霜霉病和白粉病胁迫下的表达分析[J]. 中国农业科学, 2022, 55(19): 3751-3766.