饲粮添加酿酒酵母和地衣芽孢杆菌对绵羊生长性能与瘤胃发酵的影响

doi:10.3864/j.issn.0578-1752.2020.16.015

中国农业科学 ›› 2020, Vol. 53 ›› Issue (16): 3385-3393.doi: 10.3864/j.issn.0578-1752.2020.16.015

• 畜牧·兽医·资源昆虫 • 上一篇下一篇

饲粮添加酿酒酵母和地衣芽孢杆菌对绵羊生长性能与瘤胃发酵的影响

郑玮才¹(),郝小燕¹,张宏祥²,项斌伟³,张文佳³,张春香¹,张建新¹()

¹山西农业大学动物科技学院,山西太谷 030801
²山西祥和岭上农牧开发有限公司,山西右玉037200
³山西省右玉县畜牧局,山西右玉 037200

收稿日期:2019-09-12 接受日期:2019-12-05 出版日期:2020-08-16 发布日期:2020-08-27
通讯作者: 张建新
作者简介:郑玮才,Tel：18404983839;E-mail：928156387@qq.com
基金资助:
国家重点研发计划(2018YFD0502104);现代农业产业技术体系建设专项资金(CARS-38);山西省“1331”工程建设项目(J201811301);山西省肉羊繁育工程技术中心项目;山西农业大学畜牧学学科建设专项课题资助

Effects of Saccharomyces Cerevisiae and Bacillus Licheniformis on Growth Performance and Rumen Fermentation in Sheep

ZHENG WeiCai¹(),HAO XiaoYan¹,ZHANG HongXiang²,XIANG BinWei³,ZHANG WenJia³,ZHANG ChunXiang¹,ZHANG JianXin¹()

¹College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, Shanxi
²Shanxi Xianghelingshang Farm Animal Husbandry Development Co, Ltd.,Youyu 037200, Shanxi
³Animal Husbandry Bureau of Youyu County, Youyu 037200, Shanxi

Received:2019-09-12 Accepted:2019-12-05 Online:2020-08-16 Published:2020-08-27
Contact: JianXin ZHANG

摘要/Abstract

摘要：

【目的】研究饲粮中添加酿酒酵母和地衣芽孢杆菌对绵羊生长性能和瘤胃发酵的影响,为酿酒酵母和地衣芽孢杆菌在肉羊饲粮中的合理应用提供理论依据。【方法】试验选用48只4月龄、体重相近（22.96±2.00）kg、健康的杜泊×小尾寒羊杂交F₁代公羔,根据饲喂添加剂不同随机分为4组：D（对照组,两种菌均不添加）;D1（酿酒酵母,6×10¹⁰CFU/kg）;D2（地衣芽孢杆菌,2×10¹⁰CFU/kg）;D3（酿酒酵母6×10¹⁰CFU/kg+地衣芽孢杆菌2×10¹⁰CFU/kg）,每组12只羊,试验期共75 d,前15 d为适应期,正饲期60d。试验羊每天分别于08:00和18:00进行饲喂,自由采食和饮水。正饲期内每天准确称量记录每只试验羊的喂料量和剩料量,并在第1、30、60 天晨饲前对试验羊进行称重,计算平均日采食量、平均日增重（ADG）及料重比（F/G）。试验结束当天08：00正常饲喂试验羊,3 h后采集瘤胃液,测定发酵参数、消化酶活性以及功能微生物。【结果】1）饲粮中添加酿酒酵母和地衣芽孢杆菌对试验羊的初始体重和终末体重及平均采食量的影响均不显著（P>0.05）,D3组的ADG显著高于D组（P<0.05）,D3组的F/G显著低于D组（P<0.05）,D1和D2组的F/G差异不显著（P>0.05）;2）饲粮中添加酿酒酵母和地衣芽孢杆菌对瘤胃液pH、丁酸浓度及乙丙比的影响均不显著（P>0.05）,D3组的NH₃-N浓度显著低于D组（P<0.05）,TVFA和丙酸浓度显著高于D组（P<0.05）,且D3组与D1和D2组均差异不显著（P>0.05）,D3和D2组的乙酸浓度显著高于D1与D组（P<0.05）;3）D3组的β-葡萄糖苷酶、羟甲基纤维素酶、木聚糖酶及淀粉酶活性均显著高于其他3组（P<0.05）。D1和D2组的β-葡萄糖苷酶、果胶酶、羟甲基纤维素酶及淀粉酶活性与D组无显著差异（P>0.05）。D3组蛋白酶活性显著高于D和D2组（P<0.05）,与D1组差异不显著（P>0.05）,D1和D2组的β-葡萄糖苷酶、果胶酶、羟甲基纤维素酶、木聚糖酶、蛋白酶及淀粉酶活性均差异不显著（P>0.05）;4）添加酿酒酵母和地衣芽孢杆菌对黄色瘤胃球菌、栖瘤胃普雷沃氏菌、嗜淀粉瘤胃杆菌和原虫数量的影响不显著（P>0.05）,D3组的溶纤维丁酸弧菌数显著高于其他3组（P<0.05）,白色瘤胃球菌和产琥珀酸丝状杆菌显著高于D组（P<0.05）,但与D1和D2组差异不显著（P>0.05）。与对照组相比,试验组产甲烷菌数量显著降低（P<0.05）,其中D3组最低。【结论】饲粮中添加6×10¹⁰CFU/kg酿酒酵母和2×10¹⁰CFU/kg地衣芽孢杆菌均会对绵羊瘤胃发酵产生积极影响,提高了瘤胃消化酶的活性,增加了瘤胃有益菌的数量,且二者组合饲喂效果更加显著。

关键词: 酿酒酵母, 地衣芽孢杆菌, 瘤胃发酵, 酶活性, 瘤胃微生物区系, 绵羊

Abstract:

【Objective】The objective of this experiment was to study the effects of Saccharomyces cerevisiae and Bacillus licheniformis on growth performance and rumen fermentation in sheep. 【Method】A total of 48 four-month-old healthy Dorper×Small Tail Han sheep ram lambs with similar body weight (22.96±2.00 kg) were randomly selected into 4 groups: D (the control group, without both bacteria); D1 (only adding Saccharomyces cerevisiae, 6×10¹⁰CFU/kg), D2 (only adding Bacillus licheniformis, 2×10¹⁰CFU/kg), and D3 (Saccharomyces cerevisiae 6×10¹⁰CFU/kg + Bacillus licheniformis 2×10¹⁰CFU/kg), with 12 sheep of each group. The experiment was conducted over 75d, with a 15d adaptation and a 60d the formal period. The experimental sheep were fed 2 times per day (08:00 a.m., 18:00 p.m.), free ingestion and free drinking water. During the normal feeding period, each experimental sheep were recorded accurately the amount of feed and residual of weight every day. The experimental sheep were weighed before the morning feeding on the 1st, 30th and 60th day, and the average daily intake, average daily gain (ADG) and Feed/Gain (F/G) value were calculated. At 08:00 on the day of the end of the experiment, the experimental sheep were fed normally. After 3 hours, the rumen fluid was collected and the rumen fermentation, digestive enzyme activities, and functional microorganisms were determined. 【Result】The results showed as follows: (1) the addition of Saccharomyces cerevisiae and Bacillus licheniformis in the diet had no significant effect on the final body weight and average feed intake of the test sheep (P>0.05). The ADG of D3 group was significantly higher than that of D group (P<0.05), and F/G value of D3 group was significantly lower than that of D group (P<0.05). There was no significant difference between D1 and D2 groups in F/G value (P>0.05): (2) the addition of Saccharomyces cerevisiae and Bacillus licheniformis in the diet had no significant effect on the rumen pH value, the concentration of butyric acid, and the value of Acetate/Propionate (A/P). The concentration of NH₃-N for D3 group was significantly lower than D group, but the concentration of TVFA and propionic acid was significantly higher than D group (P<0.05), with no difference among three experimental groups (P>0.05). The concentration of acetic acid in D3 and D2 group was significantly higher than that in D1 and D groups (P<0.05).3)The activity of β-glucosidase, carboxymethyl cellulose, xylanase, and amylase for D3 group was significantly higher than the other groups (P<0.05). There was no significant effect in the activities of β - glucosidase, pectase, carboxymethyl cellulose and amylase among D , D1 and D2 groups (P>0.05). The activity of protease in D3 group was significantly higher than D and D2 groups (P<0.05), with no significant difference between D3 and D1 groups(P>0.05). There was no significant difference in β-glucosidase, pectase, carboxymathy cellulose, xylanase, protease and amylase activities between D1 and D2 groups (P>0.05); (4) the addition of Saccharomyces cerevisiae and Bacillus licheniformis in the diet had no significant effect on the amount of R. flavefaciens, P. ruminicola, R. amylophilus, and Protozoan(P>0.05). The amount of B.fibrisolvens for D3 group was significantly higher than the other 3 groups (P<0.05). The amount of R. albus and F. succinogensin D3 group were significantly higher than D group (P<0.05), with no significant difference among D1, D2 and D3 groups (P>0.05). Compared with the D group, the amount of Methanogensin experimental groups were significantly decreased (P<0.05), and the D3 group was lowest. 【Conclusion】In conclusion, the dietary supplementation of Saccharomyces cerevisiae (6×10¹⁰CFU/kg) and Bacillus licheniformis (2×10¹⁰CFU/kg) had positive effects on rumen fermentation, which improved rumen digestive enzyme activity and increased the number of beneficial bacteria in rumen. Moreover, the combination of Saccharomyces cerevisiae and Bacillus licheniformis achieved better effect.

Key words: Saccharomyces cerevisiae, Bacillus licheniformis, rumen fermentation, enzyme activity, rumen microflora, sheep

郑玮才,郝小燕,张宏祥,项斌伟,张文佳,张春香,张建新. 饲粮添加酿酒酵母和地衣芽孢杆菌对绵羊生长性能与瘤胃发酵的影响[J]. 中国农业科学, 2020, 53(16): 3385-3393.

ZHENG WeiCai,HAO XiaoYan,ZHANG HongXiang,XIANG BinWei,ZHANG WenJia,ZHANG ChunXiang,ZHANG JianXin. Effects of Saccharomyces Cerevisiae and Bacillus Licheniformis on Growth Performance and Rumen Fermentation in Sheep[J]. Scientia Agricultura Sinica, 2020, 53(16): 3385-3393.

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参考文献 45

[1]	张民, 刁其玉. 益生菌的营养和免疫特性及其应用. 饲料研究, 2002(10) : 6-8.
	ZHANG M, DIAO Q Y. Nutritional and immune characteristics of probiotics and their application. Feed Research, 2002(10):6-8. (in Chinese)
[2]	乔国华, 单安山. 直接饲喂微生物培养物对奶牛瘤胃发酵产甲烷及生产性能的影响. 中国畜牧兽医, 2006(5) : 11-14.
	QIAO G H, SHAN A S. The effect of different direct-fed microbial culture on methane productionin vitro and production performance in dairy cattle. Chinese Animal Husbandry and Veterinary Medicine, 2006(5):11-14. (in Chinese)
[3]	邵广, 李红宇, 黄帅, 苗树君. 酿酒酵母对奶牛瘤胃内环境及血液生化指标的影响. 中国牛业科学, 2011,37(2):24-26.
	SHAO G, LI H Y, HUANG S, MIAO S J. Effect of aspergillus oryzue on rumen degradation rate and nutrient digestibility in dairy cow. China Cattle Science, 2011,37(2):24-26.(in Chinese)
[4]	张海涛, 王加启, 卜登攀, 栾绍宇, 邓露芳, 周凌云, 周振峰, 魏宏阳, 孙鹏. 日粮中添加纳豆枯草芽孢杆菌对犊牛消化道发育的影响. 中国畜牧兽医, 2010,37(1):5-9.
	ZHANG H T, WANG J Q, BO D P, LUAN S Y, DENG L F, ZHOU L Y, ZHOU Z F, WEI H Y, SUN P. Effect of supplementation of Bacillus subtilis Natto on the development of digestive tract in Calves. Chinese Animal Husbandry and Veterinary Medicine, 2010,37(1):5-9.(in Chinese)
[5]	郑玮才, 郝小燕, 张春香, 项斌伟, 张文佳, 温灏宇, 张建新. 酿酒酵母和地衣芽孢杆菌对绵羊瘤胃体外发酵的影响. 中国畜牧兽医, 2019,46(11):3208-3215.
	ZHENG W C, HAO X Y, ZHANG C X, XIANG B W, ZHANG W J, WEN H Y, ZHANG J X. Effects of Saccharomyces Cerevisiae and Bacillus Licheniformis on rumen fermentation in sheep in vitro. Chinese Animal Husbandry and Veterinary Medicine, 2019,46(11):3208-3215. (in Chinese)
[6]	AOAC. Official methods of analysis of AOAC International[M]. 17th ed.Gaithersburg:AOAC International, 2000.
[7]	VAN SOEST P J, ROBERTSON J B, LEWIS B A. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 1991,74(10):3583-3597. pmid: 1660498
[8]	邵俊. 干、湿法消解-火焰原子吸收法测定多种食品中钙元素的含量. 化学工程师, 2015(11):22-25.
	SHAO J. Determination of the calcium content in foods by dry ssh and wet digestion-flame atomic absorption spectrophotometry. Chemical Engineer, 2015(11):22-25. (in Chinese)
[9]	李会娟. 2种植物磷含量的检测方法比较研究. 现代农业科技, 2012(11):16-17.
	LI H J. Comparative study on determination of phosphorus content in two kinds of plants. Modern Agricultural Science and Technology, 2012(11):16-17. (in Chinese)
[10]	WANG C, LIU Q, GUO G, HUO W J, MA L, ZHANG Y L, PEI C X, ZHANG L S, WANG H. Effects of rumen-protected folic acid on ruminal fermentation, microbial enzyme activity, cellulolytic bacteria and urinary excretion of purine derivatives in growing beef steers. Animal Feed Science & Technology, 2016,221:185-194.
[11]	金亚倩, 赵俊星, 刘文忠, 任有蛇, 张春香, 张文佳, 项斌伟, 张建新. 酿酒葡萄皮渣对绵羊瘤胃代谢及发育的影响. 畜牧兽医学报, 2017,48(9):1683-1693.
	JIN Y Q, ZHAO J X, LIU W Z, REN Y S, ZHANG C X, ZHAGN W J, XIANG B W, ZHAGN J X. Effect of dietary wine grape pomace supplementation on rumen metabolism and development in lambs. Chinese Journal of Animal and Veterinary Sciences, 2017,48(9):1683-1693. (in Chinese)
[12]	AGARWAL N, KAMRA D N, CHAUDHARY L C, AGARWAL I, SAHOO A, PATHAK N N. Microbial status and rumen enzyme profile of crossbred calves fed on different microbial feed additives. Letters in Applied Microbiology, 2002,34(5):329-336. pmid: 11967054
[13]	YU Z, MORRISON M. Improved extraction of PCR-quality community DNA from digesta and fecal samples. Biotechniques, 2004,36(5):808-812. pmid: 15152600
[14]	DENMAN S E, MCSWEENEY C S. Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the ruminal. FEMS Microbiology Ecology, 2006,58(3):572-582. pmid: 17117998
[15]	KOIKE S, KOBAYASHI Y. Development and use of competitive PCR assays for the ruminal cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcusalbus and Ruminococcus flavefaciens. FEMS Microbiology Letters, 2001,204(2):361-366. pmid: 11731149
[16]	FORSTER R, TEATHER R, GONG J, DENG S J. 16s rDNA analysis of Bufyrivibriofibrisolvens: phylogenetic position and relation to butyrate‐producing anaerobic bacteria from the ruminal of white‐tailed deer. Letters in Applied Microbiology, 1996,23(4):218-222. pmid: 8987694
[17]	JAMI E, MIZRAHI I. Composition and similarity of bovine ruminal microbiota across individual animals. PLoS One, 2012,7(3):306.
[18]	ZHANG C M, GUO Y Q, YUAN Z P, WU Y M, WANG J K, LIU J X, ZHU W Y. Effect of octadeca carbon fatty acids on microbiota fermentation, methanogenesis and microbiota flora in vitro. Animal Feed Science and Technology, 2008,146(3-4):259-269.
[19]	闫晓刚. 酵母培养物和颗粒精料对荷斯坦犊牛生长发育的影响[D]. 吉林:吉林农业大学, 2005. pmid: 11615210
	YAN X G. The effect of yeast culture and pellet concentrate on the growing development of Holstein calves[D]. Jilin: Jilin Agricultural University, 2005. (in Chinese) pmid: 11615210
[20]	李栋, 姜宁. 益生素在反刍动物生产中的应用. 现代畜牧科技, 2015(5):149.
	LI D, JIANG N. Application of probiotics in ruminant production. Technical Advisor for Animal Husbandry, 2015(5):149. (in Chinese)
[21]	ZHANG H L, LI W S, XU D N, ZHENG W W. Mucosa-reparing and microbiota-balancing therapeutic effect of Bacillus subtilis alleviates dextrate sulfate sodium-induced ulcerative colitis in mice. Experimental and Therapeutic Medicine, 2016. DOI: 10.3892/etm. 2016.3686. pmid: 32782506
[22]	耿春银. 活性酵母与酵母培养物饲喂育肥牛生长性能、胴体指标和牛肉品质的比较[D]. 北京:中国农业大学, 2015.
	GENG C Y. Comparison of live yeast (Saccharomyces cerevisiae) and yeastculture for growth performance, carcass traits and meatquality in finishing cattle[D]. Beijing: China Agricultural University, 2015. (in Chinese)
[23]	董晓丽. 益生菌的筛选鉴定及其对断奶仔猪、犊牛生长和消化道微生物的影响[D]. 北京:中国农业科学院, 2013.
	DONG X L. Identification of probiotics and effects of probiotics on weaned piglets, calves and the gastrointestinal microbiate[D]. Beijing: Chinese Academy of Agricultural Sciences, 2013. (in Chinese)
[24]	FRANCIA A D, MASUCCI F, ROSA G D, VARRICCHIO M L, PROTO V. Effects of Aspergillus oryzae extract and a Saccharomyces cerevisiae fermentation product on intake, body weight gain and digestibility in buffalo calves. Animal Feed Science and Technology, 2008,140(1-2):67-77. doi: 10.1016/j.anifeedsci.2007.02.010
[25]	符运勤, 刁其玉, 屠焰, 王建红, 许先查. 不同组合益生菌对0~8周龄犊牛生长性能及血清生化指标的影响. 动物营养学报, 2012,24(4):753-761. doi: 10.3969/j.issn.1006-267x.2012.04.021
	FU Y Q, DIAO Q Y, TU Y, WANG J H, XU X C. Effects of different combinations of probiotics on growth performance and serum biochemical parameters in dairy calves aged from 0 to 8 weeks. Chinese Journal of Animal Nutrition, 2012,24(4):753-761.(in Chinese) doi: 10.3969/j.issn.1006-267x.2012.04.021
[26]	庞德公. 枯草芽孢杆菌、产朊假丝酵母与屎肠球菌对奶牛瘤胃微生物消化代谢和甲烷排放的影响[D]. 乌鲁木齐:新疆农业大学, 2014.
	PANG D G. Effects of bacillus subtilis, Candida utilis and Enterococcus faecium on in vitro rumen microbial digestion and metabolism and methane emission of dairy cows[D]. Urumqi: Xinjiang Agricultural University, 2014. (in Chinese)
[27]	陈亮, 揭红东, 任傲, 周传社, 谭支良, 李斌. 酿酒酵母对湘中黑牛营养物质消化率、瘤胃发酵及血浆生化指标的影响. 动物营养学报, 2017,29(9):3359-3365.
	CHEN L, JIE D H, REN A, ZHOU C S, TAN Z L, LI B. Effects of Saccharomyces cerevisiaeon nutrient digestibility, rumen fermentation and plama biochemical parameters of Xiangzhong black beef. Chinese Journal of Animal Nutrition, 2017,29(9):3359-3365. (in Chinese)
[28]	丁洪涛, 夏冬华, 秦珊珊, 杨新艳. 枯草芽孢杆菌对奶牛体外瘤胃发酵的影响. 饲料研究, 2012(1):57-59.
	DING H T, XIA D H, QIN S S, YANG X Y. Effect of Bacillus subtilis on rumen fermentation of dairy cattle in vitro. Feed Research, 2012(1):57-59. (in Chinese)
[29]	肖宇, 王利华, 程明, 祁茹, 褚永康, 林英庭. 功能性寡糖对奶山羊瘤胃发酵功能的影响. 动物营养学报, 2011(12) :2203-2209. doi: 10.3969/j.issn.1006-267x.2011.12.024
	XIAO Y, WANG L H, CHENG M, QI R, CHU Y K, LIN Y T. Functional oligosaccharides affect rumen fermentation of dairy goats. Chinese Journal of Animal Nutrition, 2011(12):2203-2209. (in Chinese) doi: 10.3969/j.issn.1006-267x.2011.12.024
[30]	SMAGALA A M . The effect of preservatives based on propionic acid on the fermentation and aerobic stability of corn silage and a total mixed ration. Journal of Dairy Science, 1998,81(5):1322-1330. pmid: 9621235
[31]	WILLIAMS P E V, NEWBOLD C J, GAMSWORTHY P C. Rumen probiosis: the effects of novel microorganisms on rumen fermentation and ruminant productivity. Recent Advances in Animal Nutrition, 1990: 211-227.
[32]	PROHASZKA' L, JAYARAO B M., FABIAN'A , KOVACS' S. The role of intestinal volatile fatty acids in the Salmonella shedding of pigs. ZentralblattFürVeterinärmedizin.reiheB. Journal of Veterinary Medicine, 2010,37(1-10):570-574.
[33]	肖怡. 三种益生菌对肉羊甲烷排放、物质代谢和瘤胃发酵的影响[D]. 塔里木:塔里木大学, 2016.
	XIAO Y. Effects of three probiotics on methane emission, nutrient metabolism and rumen fermentation in mutton sheep[D]. Aral: Tarim University, 2016. (in Chinese)
[34]	李鹤琼, 刘强, 王聪, 张延利, 裴彩霞, 王永新, 郭刚, 霍文婕, 张拴林, 刘建新. 2-甲基丁酸对断奶前后犊牛瘤胃发酵、酶活及纤维分解菌菌群的影响. 畜牧兽医学报, 2015,46(12):2218-2226. doi: 10.11843/j.issn.0366-6964.2015.12.013
	LI H Q, LIU Q, WANG C, ZHANG Y L, PEI C X, WANG Y X, GUO G, HUO W J, ZHANG S L, LIU J X. Effects of 2 methylbutyrate supplementation on rumen fermentation, enzyme activities and cwllulolytic bacteria in pre and post weaning dairy calves. Chinese Journal of Animal and Veterinary Sciences, 2015,46(12):2218-2226. (in Chinese) doi: 10.11843/j.issn.0366-6964.2015.12.013
[35]	EMMANUEL D G, JAFARI A, BEAUCHEMIN K A, LEEDLE J A Z, AMETAJ B N. Feeding live cultures of Enterococcus faecium and Saccharomyces cerevisiae induces an inflammatory response in feedlot steers. Journal of Animal Science, 2007,85(1):233-239. doi: 10.2527/jas.2006-216 pmid: 17179561
[36]	DAWSON K A, NEWMAN K E, BOLING J A. Effects of microbial supplements containing yeast and lactobacilli on roughage-fed ruminal microbial activities. Journal of Animal Science, 1990,68(10):3392-3398. pmid: 2123850
[37]	于萍, 王加启, 卜登攀, 刘开朗, 李旦, 赵圣国, 魏宏阳, 周凌云. 日粮添加纳豆芽孢杆菌对断奶后犊牛胃肠道纤维分解菌的影响. 中国农业大学学报, 2009,14(1):111-116.
	YU P, WANG J Q, BO D P, LIU K L, LI D, ZHAO S G, WEI H Y, ZHOU L Y. Effects ofBacillus subtilis natto in diets on quantities of gastrointestinal cellulytic bacteria in weaning calves. Journal of China Agricultural University, 2009,14(1):111-116. (in Chinese)
[38]	黄庆生, 王加启. 添加不同酵母培养物对瘤胃纤维分解菌群和纤维素酶活的影响. 畜牧兽医学报, 2005,36(2):144-148.
	HUANG Q S, WANG J Q. Effect of yeast cultures on fibrolyticbacterial population and activities of fiber hydrolytic enzymes in the rumen. Chinese Journal of Animal and Veterinary Sciences, 2005,36(2):144-148. (in Chinese)
[39]	黄帅. 米曲霉和酿酒酵母对奶牛瘤胃发酵及血液生化指标的影响[D]. 大庆: 黑龙江八一农垦大学, 2011.
	HUANG S. The effects of Aspergillus Oryzae and Saccharomyces cerevisiae on the rumen fermentation and the blood parameters of dairy cow[D]. Daqing: Heilongjiang Bayi Agricultural University, 2011. (in Chinese)
[40]	纪宁, 孔繁东, 祖国仁, 季瑛, 黄朝明. 纳豆菌抗菌作用的研究现状与展望. 食品研究与开发, 2006,27(1):138-141.
	JI N, KONG F D, ZU G R, JI Y, HUANG C M. Thepresent situatin and developmental tendency of antimicrobial function of Bacillusnatto. Food Research and Developmen, 2006,27(1):138-141. (in Chinese)
[41]	STUMM C K, GIJZEN H J, VOGELS G D. Association of methanogenic bacteria with ovine rumen ciliates. Applied & Environmental Microbiology, 1982,47(1):95-99.
[42]	PLATAP F, MENDOZA M GD, BFIRCENA-GAMA JR, GONZFILEZ M S. Effect of a yeast culture (Saccharomyces cerevisiae) on neutral detergent fiber digestion in steers fed oat straw based diets. Animal Feed Science & Technology, 1994,49(3-4):203-210.
[43]	YOON I K, STERM M D. Effects of Saccharomyces cerevisiae and Aspergillus oryzae cultures on ruminal fermentation in dairy cows. Journal of Dairy Science, 1996,79(3):411. doi: 10.3168/jds.S0022-0302(96)76380-4 pmid: 8708102
[44]	NEWBOLD C J, WALLACE R J, CHEN X B, MCLNTOSH F M. Different strains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in vitro and in sheep. Journal of Animal Science, 1995,73(6):1811-1818. doi: 10.2527/1995.7361811x pmid: 7673076
[45]	孙鹏. 日粮添加纳豆枯草芽孢杆菌对奶牛生产性能、瘤胃发酵及功能微生物的影响. 中国畜牧兽医, 2012(9):168.
	SUN P. Effects ofBacillus subtilis natto on performance, rumen fermentation and functional microorganisms in dairy cows. Chinese Animal Husbandry and Veterinary Medicine, 2012(9):168. (in Chinese)

项目 Item		营养水平 Nutrient level
原料 Ingredients		干物质 DM	94.99
玉米 Corn	30.00	粗蛋白质 CP	13.14
米糠 Rice bran	7.00	粗灰分 Ash	8.27
豆粕 Soybean meal	18.00	粗脂肪 EE	2.23
预混料 Premix¹	5.00	中性洗涤纤维 NDF	41.84
玉米秸秆 Corn straw	20.00	酸性洗涤纤维 ADF	22.29
谷草 Millet straw	20.00	钙 Ca	0.35
合计 Total	100.00	磷 P	0.29

基因 Gene name	引物序列（5'→3'）Primer sequence(5'→3')	产物大小 Product/bp	参考文献 References
总菌 Total bacterial	F:CGGTGAATACGTTCYCGG	123	DENMAN等^[14]
总菌 Total bacterial	R:GGWTACCTTGTTACGACTT	123	DENMAN等^[14]
黄色瘤胃球菌 flavefaciens	F:ATTGTCCCAGTTCAGATTGC	173	DENMAN等^[14]
黄色瘤胃球菌 flavefaciens	R:GGCGTCCTCATTGCTGTTAG	173	DENMAN等^[14]
白色瘤胃球菌 albus	F:CCCTAAAAGCAGTCTTAGTTCG	176	KOIKE等^[15]
白色瘤胃球菌 albus	R:CCTCCTTGCGGTTAGAACA	176	KOIKE等^[15]
产琥珀酸丝状杆菌 succinogens	F:GGCGGGATTGAATGTACCTTGAGA	204	DENMAN等^[14]
产琥珀酸丝状杆菌 succinogens	R:TCCGCCTGCCCCTGAACTATC	204	DENMAN等^[14]
溶纤维丁酸弧菌 fibrisolvens	F:TAACATGAGAGTTTGATCCTGGCTC	136	FORSTER等^[16]
溶纤维丁酸弧菌 fibrisolvens	R:CGTTACTCACCCGTCCGC	136	FORSTER等^[16]
嗜淀粉瘤胃杆菌 R. amylophilus	F:CTGGGGAGCTGCCTGAATG	100	JAMI等^[17]
嗜淀粉瘤胃杆菌 R. amylophilus	R:GCATCTGAATGCGACTGGTTG	100	JAMI等^[17]
栖瘤胃普雷沃氏菌 P. ruminicola	F:GAAAGTCGGATTAATGCTCTATGTTG	74	JAMI等^[17]
栖瘤胃普雷沃氏菌 P. ruminicola	R:CATCCTATAGCGGTAAACCTTTGG	74	JAMI等^[17]
产甲烷菌 Methanogens	F:TTCGGTGGATCDCARAGRGC	140	ZHANGI等^[18]
产甲烷菌 Methanogens	R:GBARGTCGWAWCCGTAGAATCC	140	ZHANGI等^[18]
原虫 Protozoan	F:GCTTTCGWTGGTAGTGTATT	223	ZHANGI等^[18]
原虫 Protozoan	R:CTTGCCCTCYAATCGTWCT	223	ZHANGI等^[18]

项目 Items	组别Groups				SEM	P值 P value
项目 Items	D	D1	D2	D3	SEM	P值 P value
初始体重IBW (kg)	23.08	22.22	23.58	22.97	1.20	0.73
终末体重FBW (kg)	39.91	40.04	40.92	43.23	2.77	0.61
平均日增重ADG (g·d^-1)	280.42d	297.38b	289.34c	337.40a	35.22	0.01
平均日采食量ADFI (g·d^-1)	1827.11	1768.90	1759.31	1705.04	165.49	0.91
料重比F/G	6.57a	5.98ab	6.02ab	5.01b	0.27	0.02

项目 Items	组别Groups				SEM	P值 P value
项目 Items	D	D1	D2	D3	SEM	P值 P value
pH	6.76	6.58	6.52	6.74	0.14	0.11
氨态氮NH₃-N (mg·dL^-1)	25.09a	22.94ab	23.02ab	19.87b	1.60	0.03
总挥发性脂肪酸TVFA (mmol·L^-1)	48.03b	51.81ab	54.37a	54.86a	2.22	0.02
乙酸Acetate (mmol·L^-1)	35.25c	38.01b	39.69a	40.09a	0.58	<0.001
丙酸Propionate (mmol·L^-1)	11.44b	12.07ab	12.99a	13.39a	0.51	0.004
丁酸Butyrate (mmol·L^-1)	6.67	6.94	6.78	6.92	0.66	0.97
乙丙比Acetate/Propionate	3.08	3.15	3.07	3.01	0.13	0.69

项目 Items	组别Groups				SEM	P值 P value
项目 Items	D	D1	D2	D3	SEM	P值 P value
β-葡萄糖苷酶β-glucosidase	0.08b	0.09b	0.09b	0.17a	0.01	<0.001
果胶酶Pectase	0.62b	0.73ab	0.88b	0.93a	0.08	0.002
羟甲基纤维素酶Carboxymethyl cellulose	0.09b	0.15b	0.16b	0.25a	0.03	0.001
木聚糖酶 Xylanase	0.23c	0.26bc	0.29b	0.46a	0.01	<0.001
蛋白酶 Protease	15.77c	19.54ab	17.43bc	21.21a	0.11	<0.001
淀粉酶Amylase	0.24b	0.25b	0.26b	0.30a	0.01	0.002

饲粮添加酿酒酵母和地衣芽孢杆菌对绵羊生长性能与瘤胃发酵的影响

Effects of Saccharomyces Cerevisiae and Bacillus Licheniformis on Growth Performance and Rumen Fermentation in Sheep

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项目 Items	组别Groups				SEM	P值 P value
项目 Items	D	D1	D2	D3	SEM	P值 P value
白色瘤胃球菌R. albus	0.68b	1.59ab	2.08a	2.54a	0.37	0.001
产琥珀酸丝状杆菌F. succinogenes	6.53b	10.49ab	9.27ab	15.05a	2.59	0.04
黄色瘤胃球菌R. flavefaciens	3.29	3.43	4.34	5.19	1.04	0.27
溶纤维丁酸弧菌B. fibrisolvens	9.47b	10.66b	10.45b	13.49a	0.46	<0.001
栖瘤胃普雷沃氏菌P. ruminicola	1.55	2.06	2.57	2.59	0.57	0.26
嗜淀粉瘤胃杆菌R. amylophilus	0.90	1.11	0.98	1.33	0.18	0.20
产甲烷菌Methanogens×10^-2	29.19a	18.74bc	20.26b	11.62c	2.08	<0.001
原虫Protozoan	6.44	5.74	5.84	5.49	1.38	0.94

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