中国农业科学 ›› 2018, Vol. 51 ›› Issue (24): 4677-4689.doi: 10.3864/j.issn.0578-1752.2018.24.008
收稿日期:
2018-04-16
接受日期:
2018-07-10
出版日期:
2018-12-16
发布日期:
2018-12-16
基金资助:
GUO Jing(),LUO PeiYu(
),YANG JinFeng,LI DongDong,HUANG YueYue,HAN XiaoRi(
)
Received:
2018-04-16
Accepted:
2018-07-10
Online:
2018-12-16
Published:
2018-12-16
摘要:
目的 丛枝菌根(Arbuscular mycorrhizal, AM)真菌有改善根际土壤环境、促进植物对养分的吸收、增强植物抗逆性和增加农作物产量等重要作用。本研究旨在探明长期施肥条件下玉米-大豆轮作棕壤丛枝菌根真菌群落结构、对玉米根系侵染的变化及其影响因素。方法 以沈阳农业大学棕壤肥料长期定位试验(38年)耕层(0—20 cm)土壤为材料,于2016年6月选取其中6个施肥处理:(1)不施肥处理(CK);(2)单施化学氮肥(N);(3)施用化学氮磷肥(NP);(4)施用化学氮磷钾肥(NPK);(5)单施有机肥(M);(6)有机肥和化学氮磷肥配施(MNP),采用PCR-DGGE、克隆测序及台盼蓝染色法,分析土壤和玉米根系定殖的AM真菌群落结构及侵染率,并结合环境因素进行冗余分析和典型对应分析。结果 施用有机肥处理土壤的碱解氮(AHN)、速效磷(AP)、速效钾(AK)、铵态氮(NH4 +-N)、硝态氮(NO3 --N)和可溶性有机碳(DOC)含量显著高于单施化肥和不施肥处理,且趋势为:有机肥处理>化肥处理>不施肥处理;与不施肥处理相比,单施化肥处理显著降低了土壤pH值,而施用有机肥处理显著提高了土壤pH值。通过PCR-DGGE及割胶测序,从土壤中得到AM真菌条带22条,根系AM真菌条带仅9条,共分离出13个OTU,从土壤样品中分离的AM真菌种群主要为球囊霉菌和巨孢囊霉属,而侵染玉米根系的AM真菌只有球囊霉菌。聚类分析表明长期不同施肥将棕壤中AM真菌分为了三大类群,分别为单施氮肥处理、施用有机肥处理和其他处理;根系AM真菌分为三大类群,第一类群NPK处理、第二类群为M处理和NP处理、第三类群为其他施肥处理。施用有机肥处理AM真菌的孢子密度显著高于单施化肥和不施肥处理,趋势为:有机肥处理﹥化肥处理﹥不施肥处理。各施肥处理AM真菌侵染率趋势为:NPK处理>施用有机肥处理>其他施肥处理。冗余分析结果表明棕壤AM真菌多样性与土壤理化性质无相关性,而孢子密度与土壤AHN、NH4 +-N、AP、AK、DOC及土壤含水量呈显著正相关;侵染率与土壤NO3 --N呈显著正相关;侵染率与孢子密度之间呈显著正相关;AM真菌的多样性与孢子密度和侵染率之间没有相关性。典型对应分析表明AHN、AK、DOC、 NH4 +-N 对AM真菌的群落组成影响显著。 结论 长期施肥通过改变土壤理化性质,从而对棕壤AM真菌的群落结构产生了显著影响,进而对AM真菌的侵染产生影响。
郭静,罗培宇,杨劲峰,李冬冬,黄月玥,韩晓日. 长期施肥对棕壤丛枝菌根真菌群落结构及其侵染的影响[J]. 中国农业科学, 2018, 51(24): 4677-4689.
GUO Jing,LUO PeiYu,YANG JinFeng,LI DongDong,HUANG YueYue,HAN XiaoRi. Influence of Long-term Fertilization on Community Structures and Colonization of Arbuscular mycorrhizal Fungi in a Brown Soil[J]. Scientia Agricultura Sinica, 2018, 51(24): 4677-4689.
表1
AM真菌目的序列PCR反应条件"
引物名称Primer | 引物序列(5′-3′)Primer sequence(5′-3′) | 反应条件Conditions |
---|---|---|
NS1 | GTAGTCATATGCTTGTCTC | 94℃预变性3 min;94℃ 1 min,50 ℃ 1 min,72 ℃ 1 min,30个循环;72 ℃ 7 min (一轮) An initial denaturation of 94℃for 3 min; 94℃ 1 min, 50℃ 1 min, 72℃ 1 min, 30 cycles; 72℃ 7 min (The first round) |
NS41 | CCCGTGTTGAGTCAAATTA | |
GC-NS31 | TTGGAGGGCAAGTCTGGTGCC | |
AM1 | GTTTCCCGTAAGGCGCCGAA | 94 ℃预变性3 min;94 ℃ 30 s,58 ℃ 45 s,72 ℃ 45 s,28个循环;72 ℃ 7 min(二轮) An initial denaturation of 94℃ for 3 min; 94℃ 30 s, 58℃ 45 s, 72℃ 45 s, 28 cycles; 72℃ 7 min (The second round) |
GC-ARCH1311 | TGCTAAATAGCCAGGCTGY | |
NS8 | TCCGCAGGTTCACCTACGGA |
表2
供试土壤的理化性质"
处理 Treatment | pH (H2O) | 速效磷 AP (mg·kg-1) | 速效钾 AK (mg·kg-1) | 碱解氮 AHN (mg·kg-1) | 硝态氮 NO3--N (mg·kg-1) | 铵态氮 NH4+-N (mg·kg-1) | 可溶性有机碳 DOC (mg·kg-1) | 土壤含水量 SM (%) |
---|---|---|---|---|---|---|---|---|
CK | 5.75±0.01b | 1.19±0.64d | 96.66±1.21c | 85.04±0.68f | 12.06±0.02e | 3.97±0.18d | 39.42±0.26e | 15.40±0.14b |
N | 4.87±0.00d | 2.14±0.64d | 81.62±1.86c | 110.43±0.94d | 48.38±1.03c | 4.45±0.12cd | 65.41±0.05d | 15.41±0.22b |
NP | 5.35±0.01c | 16.15±0.22c | 76.94±2.32c | 96.60±1.17e | 51.22±1.39bc | 4.83±0.16bc | 71.29±0.74c | 15.12±0.38b |
NPK | 4.99±0.01c | 17.10±0.63c | 107.02±1.16c | 120.73±2.43c | 55.97±0.74b | 5.26±0.046b | 73.10±0.17c | 15.80±0.04b |
M | 6.64±0.06a | 137.72±2.29b | 209.96±9.98b | 132.54±1.74b | 41.81±0.75d | 5.33±0.09b | 108.44±2.51b | 18.11±0.04a |
MNP | 5.78±0.02b | 182.15±7.01a | 268.12±9.75a | 165.56±2.24a | 73.63±0.43a | 7.75±0.12a | 126.45±1.30a | 18.20±0.73a |
[1] | 杨凤铃, 赵方贵, 刘洪庆, 刘新 . 不同烟草栽培地区土壤理化性质与AM真菌分布关系. 中国农学通报, 2011,27(1):116-120. |
YANG F L, ZHAO F G, LIU H Q, LIU X . Relationships between physical and chemical properties of different tobacco cultivation soils and distribution of AM Fungi. Chinese Agricultural Science Bulletin, 2011,7(1):116-120. (in Chinese) | |
[2] | 黄京华, 曾任森, 骆世明 . AM 菌根真菌诱导对提高玉米纹枯病抗性的初步研究. 中国生态农业学报, 2006,14(3):167-169. |
HUANG J H, ZENG R S, LUO S M . Studies on disease resistance of maize toward sheath blight induced by arbuscular mycorrhizal fungi. Chinese Journal of Eco-Agriculture, 2006,14(3):167-169. (in Chinese) | |
[3] | GUPTA M L, PRASAD A, RAM M, KUMAR S . Effect of the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus fasciculatum, on the essential oil yield related characters and nutrient acquisition in the crops of different cultivars of menthol mint(Mentha arvensis ) under field conditions. Bioresource Technology, 2002,81(1):77-79. |
[4] | 冯海艳, 冯固, 宋建兰, 王敬国, 李晓林 . 丛枝菌根真菌根内菌丝碱性磷酸酶活性与菌根共生效应的研究. 中国生态农业学报, 2004,12(2):124-127. |
FENG H Y, FENG G, SONG J L, WANG J G, LI X L . Studies on the relationship between the activity of alkaline phosphatase in intraradical hyphae of arbuscular mycorrhizae fungi and efficiency of mycorrhizal symbiosis. Chinese Journal of Eco-Agriculture, 2004,12(2):124-127. (in Chinese) | |
[5] | 刘翠花, 陈保冬, 朱永官, 张澈 . 丛枝菌根真菌对青稞生长发育及磷营养的影响研究. 土壤学报, 2006,43(6):1052-1055. |
LIU C H, CHEN B D, ZHU Y G, ZHANG C . Effects on arbuscular mycorrhizal fungi on growth and phosphorus nutrition of Highland Barley. Acta Pedologica Sinica, 2006,43(6):1052-1055. (in Chinese) | |
[6] | 张燕, 李娟, 姚青, 陈杰忠, 胡又厘 . 枇杷质膜水孔蛋白基因EjPIP1的克隆及AM真菌对其表达的影响. 中国农业科学, 2014,47(7):1387-1396. |
ZHANG Y, LI J, YAO Q, CHEN J Z, HU Y L . Cloning of a plasma membrane aquaporin gene EjPIP1 in Eriobotrya japonica leaves and its expression analysis after AM fungi inoculation. Scientia Agricultura Sinica, 2014,47(7):1387-1396. (in Chinese) | |
[7] | GOSLING P, HODGE A, GOODLASS G, BENDING G D . Arbuscular mycorrhizal fungi and organic farming. Agriculture Ecosystems & Environment, 2005,113(1):17-35. |
[8] | DIAZ G, ROLDAN A, ALBALADEJO J . Soil type as affecting colonization patterns and mycorrhizal symbiosis effectiveness of 6 glomus species. Cryptogamie Mycologie, 1992,13(1):47-56. |
[9] | 张海波, 梁月明, 冯书珍, 赵紫薇, 苏以荣, 何寻阳 . 土壤类型和树种对根际土丛枝菌根真菌群落及其根系侵染率的影响. 农业现代化研究 , 2016,37(1):187-194. |
ZHANG H B, LIANG Y M, FENG S Z, ZHAO Z W, SU Y R, HE X Y . The effects of soil types and plant species on arbuscular mycorrhizal fungi community and colonization in the rhizosphere. Research of Agricultural Modernization, 2016,37(1):187-194. (in Chinese) | |
[10] | 闫飞扬, 段廷玉, 张峰 . 农业管理措施对AM真菌功能影响的研究进展. 草业科学, 2014,31(12):2230-2241. |
YAN F Y, DUAN T Y, ZHANG F . Effects of agricultural managements on the function of arbuscular mycorrhizal fungi. Pratacultural Science, 2014,31(12):2230-2241. (in Chinese) | |
[11] | 田慧, 盖京苹, 李晓林, 张俊伶 . 农田土著丛枝菌根真菌群落特征和磷吸收作用研究进展. 土壤通报, 2013,44(6):1512-1519. |
TIAN H, GAI J P, LI X L, ZHANG J L . Community composition and phosphorus uptake by indigenous arbuscular mycorrhizal fungi in agroecosystems. Chinese Journal of Soil Science, 2013,44(6):1512-1519. (in Chinese) | |
[12] | 秀彦华, 刘润进, 李敏 . 自然入侵条件下黄顶菊丛枝菌根定殖及发育的研究. 菌物学报, 2015,34(1):82-90. |
XIU Y H, LIU R J, LI M . Arbuscular mycorrhizal colonization and development of Flaveria bidentis under natural invasive conditions. Mycosystema, 2015,34(1):82-90. (in Chinese) | |
[13] | 高秀兵, 陈娟, 赵华富, 李珍, 郭灿, 周富裕, 王振轩, 涂逸洋, 周玉峰 . 贵州地方种茶树根系丛枝菌根真菌的侵染特征. 西南农业学报, 2016,6(29):1328-1335. |
GAO X B, CHEN J, ZHAO H F, LI Z, GUO C, ZHOU F Y, WANG Z X, TU Y Y, ZHOU Y F . Colonization characteristics of arbuscular mycorrhiza fungi in rhizosphere of local tea trees in Guizhou. Southwest China Journal of Agricultural Sciences, 2016,6(29):1328-1335. (in Chinese) | |
[14] |
WU F, DONG M, LIU Y, MA X, AN L ,YOUNG J P W. Effects of long-term fertilization on AM fungal community structure and Glomalin-related soil protein in the Loess Plateau of China. Plant and Soil, 2011,342(1/2):233-247.
doi: 10.1007/s11104-010-0688-4 |
[15] | 张鑫, 裴宗平, 孙干, 苏雪鹏 . 紫花苜蓿根际丛枝菌根真菌与土壤理化性质的相关性研究.北方园艺, 2016(13):172-177. |
ZHANG X, PEI Z P, SUN G, Su X P . Correlation between physicochemical properties of rhizosphere soil and arbuscula mycorrhizae fungi inMedicago sativa l.Grassland. Northern Horticulture, 2016(13):172-177. (in Chinese) | |
[16] |
JONER E J . The effect of long-term fertilization with organic or inorganic fertilizers on mycorrhiza-mediated phosphorus uptake in subterranean clover. Biology and Fertility of Soils, 2000,32(5):435-440.
doi: 10.1007/s003740000279 |
[17] | 贾锐, 杨秀丽, 闫伟 . 兴安杜鹃菌根形态特征和土壤理化性质的关系研究. 内蒙古农业大学学报, 2011,32(3):63-66. |
JIA Y, YANG X L, YAN W . Study on the relationship between morphology and soil properties mycorrhizal fungi of rhododendron dauricum. Journal of Inner Mongolia Agricultural University, 2011,32(3):63-66. (in Chinese) | |
[18] |
MUCHANE M N, JAMA B, OTHIENO C, OKALEBO R, ODEE D, MACHUA J . Influence of improved fallow systems and phosphorus application on arbuscular mycorrhizal fungi symbiosis in maize grown in western Kenya. Agroforestry Systems, 2010,78(2):139-150.
doi: 10.1007/s10457-009-9249-3 |
[19] | 罗培宇, 樊耀, 杨劲峰, 葛银凤, 蔡芳芳, 韩晓日 . 长期施肥对棕壤氨氧化细菌和古菌丰度的影响. 植物营养与肥料学报, 2017,23(3):678-685. |
LUO P Y, FAN Y, YANG J F, GE Y F, CAI F F, HAN X R . Influence of long-term fertilization on abundance of ammonia oxidizing bacteria and archaea in brown soil. Journal of Plant Nutrition and Fertilizer, 2017,23(3):678-685. (in Chinese) | |
[20] |
TU C L, LIU C Q, LU X H, YUAN J, LANG Y C . Sources of dissolved organic carbon in forest soils: evidences from the differences of organic carbon concentration and isotope composition studies. Environmental Earth Sciences, 2011,63(4):723-730.
doi: 10.1007/s12665-010-0741-x |
[21] | 鲍士旦 .土壤农化分析. 3版. 北京: 中国农业出版社, 2000: 39-109. |
BAO S D. Soil and Agricultural Chemistry Analysis. 3rd ed. Beijing: China Agriculture Press, 2000: 39-109. (in Chinese) | |
[22] |
HENDRIX J W, HERSHMAN D E, HENSON G T . Evaluation of the "most probable number" (MPN) and wet-sieving methods for determining soil-borne populations of endogonaceous mycorrhizal fungi. Mycologia, 1990,82(5):576-581.
doi: 10.2307/3760048 |
[23] | 盛萍萍, 刘润进, 李敏 . 丛枝菌根观察与侵染率测定方法的比较. 菌物学报, 2011,30(4):519-525. |
SHENG P P, LIU R J, LI M . Methodological comparison of observation and colonization measurement of arbuscular mycorrhizal fungi. Mycosystema, 2011,30(4):519-525. (in Chinese) | |
[24] |
RENKER C, HEINRICHS J, KALDORF M, BUSCOT F . Combining nested PCR and restriction digest of the internal transcribed spacer region to characterize arbuscular mycorrhizal fungi on roots from the field. Mycorrhiza, 2003,13(4):191-198.
doi: 10.1007/s00572-002-0214-5 pmid: 12938031 |
[25] | HARLEY J L, SMITH S E . Mycorrhizal symbiosis. Quarterly Review of Biology, 2008,3(3):273-281. |
[26] | 李登武, 贺学礼, 余仲东 . 施钾量与AM真菌接种效应的关系. 西北植物学报, 2002,22(4):889-893. |
LI D W, HE X L, YU Z D . Amount of potassium application in relation to AM fungus inoculation efficiency. Acta Botanica Boreali-occidentalia Sinica, 2002,22(4):889-893. (in Chinese) | |
[27] | 程俐陶, 郭巧生, 刘作易 . 半夏丛枝菌根真菌侵染模式及侵染率动态变化. 贵州农业科学, 2009,37(2):37-39. |
CHEN L T, GUO Q S, LIU Z Y . Infection pattern and dynamic change of arbuscular mycorrhizal fungi in Pinellia ternate. Guizhou Agricultural Sciences, 2009,37(2):37-39. (in Chinese) | |
[28] |
BHADALUNG N N, SUWANARIT A, DELL B, NOPAMORNBODI O ,THAMCHAIPENET A.AND RUNGCHUANG J. Effects of long-term NP-fertilization on abundance and diversity of arbuscular mycorrhizal fungi under a maize cropping system. Plant and Soil, 2005,270(1):371-382.
doi: 10.1007/s11104-004-1829-4 |
[29] | 胡从从, 郭清华, 贺学礼, 赵丽莉, 李英鹏, 曹翠兰 . 蒙古沙冬青伴生植物AM真菌多样性. 西北农业学报, 2016,25(6):921-932. |
HU C C, GUO Q H, HE X L, ZAHO L L, LIN Y P, CAO C L . Diversity of arbuscular mycorrhiza fungi near to the associated plants of Ammopiptanthus mongolicus. Acta Agriculturae Boreali-occidentalis Sinica, 2016,25(6):921-932. (in Chinese) | |
[30] | 胡静, 罗充, 吴涛 . 刺梨丛枝菌根真菌的侵染特征及其种类鉴定. 贵州农业科学, 2015,43(9):82-86. |
HU J, LUO C, WU T . Infection characteristics and species identification of arbuscular mycorrhizal fungi in the rhizosphere of Rosa roxburghii Tratt. Guizhou Agricultural Sciences, 2015,43(9):82-86. (in Chinese) | |
[31] | 王茜, 王强, 王晓娟, 熊友才, 祝英, 张亮, 金樑 . 不同AM真菌对玉米生长的促生效应. 兰州大学学报, 2015,51(4):558-563. |
WANG Q, WANG Q, WANG Q J, XIONG Y C, ZHU Y, ZHANG L, JIN L . Effects of various AM fungi on the growth of corn(Zea mays). Journal of Lanzhou University, 2015,51(4):558-563. (in Chinese) | |
[32] | SMITH S E , READ D J . Mycorrhizal Symbiosis. New York: Academic Press, 2010: 11-188. |
[34] |
ELLOUZE W, HAMEL C, CRUZ A F, ISHII T, GAN Y, BOUZID S . Phytochemicals and spore germination: At the root of AMF host preference? Applied Soil Ecology, 2012,60(4):98-104.
doi: 10.1016/j.apsoil.2012.02.004 |
[33] | 邓小军, 周国英, 刘君昂, 李琳, 布婷婷 . 湖南油茶林丛枝菌根真菌多样性及其群落结构特征. 中国林业科技大学学报, 2011,31(10):38-42. |
DENG X J, ZHOU G Y, LIU J A, LI L, BU T T . Diversity and community structure of arbuscular mycorrhizal fungi in Camellia oleifera stands in Hunan. Journal of Central South University of Forestry & Technology, 2011,31(10):38-42. (in Chinese) | |
[34] | GUAA Y , LINDSTRÖM K. Soil microbial biomass, crop yields, and bacterial community structure as affected by long-term fertilizer treatments under wheat-rice cropping. European Journal of Soil Biology, 2009,45(3):239-246. |
[35] | 冯固, 杨茂秋, 白灯莎, 黄全生 . VA 菌根真菌对棉花磷素吸收及生长的效应. 西北农业学报, 1994,3(2):75-80. |
FENG G, YANG M Q, BAI D S, Huang Q S . Effect of inoculating with vescular-arbuscular mycorrgizal fungi on the p nutrient and growth of cotton. Acta Agriculturae Boreali-Occidentalis Sinica, 1994,3(2):75-80. (in Chinese) | |
[36] | 李伟, 孙龙燕, 张翠萍, 徐萌, 郭绍霞 . 濒危植物崂山百合根围AM真菌分布特征. 东北农业大学学报, 2016,47(1):30-37. |
LI W, SUN L Y, ZHANG C P, XU M, GUO S X . Distribution characteristic of arbuscular mycorrhizal fungi in the rhizosphere of endangered Lilium tsingtauense plants. Journal of Northeast Agricultural University, 2016,47(1):30-37. (in Chinese) | |
[37] | 张俊英, 王敬国, 许永利, 李海港 . 氮素对不同大豆品种根系分泌物中有机酸的影响. 植物营养与肥料学报, 2007,13(3):398-403. |
ZHANG J Y, WANG J G, XU R L, LI H G . Effect of nitrogen on the species and contents of organic acids in root exudates of different soybean cultivars. Plant Nutrition and Fertilizer Science, 2007,13(3):398-403. (in Chinese) | |
[38] |
AKIYAMA K, MATSUZAKI K, HAYASHI H . Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi. Nature, 2005,435(7043):824-827.
doi: 10.1038/nature03608 pmid: 15944706 |
[39] | 肖艳萍, 李涛, 费洪运, 赵之伟 . 云南金顶铅锌矿区丛枝菌根真菌多样性的研究. 菌物学报, 2008,27(5):652-662. |
XIAO Y P, LI T, FEI H T, ZHAO Z W . Species diversity of arbuscular mycorrizal fungi in Jinding Pb-Zn mining area of Lanping, Yunnan. Mycosystema, 2008,27(5):652-662. (in Chinese) | |
[40] |
CAMARGO-RICALDE S L, DHILLION S S . Endemic Mimosa species can serve as mycorrhizal "resource isands" within semiarid communities of the Tehuacan-Cuicatlan Valley, Mexico. Mycorrhiza, 2003,13(3):129-136.
doi: 10.1007/s00572-002-0206-5 pmid: 12836080 |
[41] | KENNEDY L J.TILLER R L, STUTZ J C . Associations between arbuscular mycorrhizal fungi and Sporobolus wrightii in riparian habitats in arid South- western North America. Journal of Arid Environment, 2002,50(3):459-475. |
[42] | 邬奇峰, 陆扣萍, 毛霞丽, 秦华, 王海龙 . 长期不同施肥对农田土壤养分与微生物群落结构的影响. 中国农学通报, 2015,31(5):150-156. |
WU Q F, LU K P, MAO X L, QIN H, WANG H L . Responses of soil nutrients and microbial biomass and community composition to long-term fertilization in cultivated land. Chinese Agricultural Science Bulletin, 2015,31(5):150-156. (in Chinese) | |
[43] |
MATHIMARAN N, RUH R, VULLIOUD P, FROSSARD E, JANSA J . Glomus intraradices dominates arbuscular mycorrhizal communities in a heavy textured agricultural soil. Mycorrhiza, 2005,16(1):61-66.
doi: 10.1007/s00572-005-0014-9 pmid: 16133255 |
[44] | BLANKE V, RENKER C, WAGNER M, FILLNER K, HELD M, KUHN A J, BUSCOT F . Nitrogen supply affects arbuscular mycorrhizal colonization of Artemisia vulgaris in a phosphate- polluted field site. New Phytologist, 2005,166(3):981-992. |
[45] | 刘权钢, 金东淳, 刘敬爱 . DGGE技术在土壤微生物多样性分析上的研究进展. 延边大学农学学报, 2012,34(2):170-176. |
LI Q G, JIN D C, LIU J A . Research progress of DGGE technique for soil microbial diversity analysis. Journal of Agricultural Science Yanbian University, 2012,34(2):170-176. (in Chinese) | |
[46] | 林先贵, 胡君利, 戴珏, 王发园, 冯有智 . 丛枝菌根真菌群落结构与多样性研究方法概述及实例比较. 应用与环境生物学报, 2017,23(2):343-350. |
LI X G, HU G L, DAI Y, WANG F Y, FNG Y Z . Overview and comparison of research methods for determining the community structure and diversity of arbuscular mycorrhizal fungi. Chinese Journal of Applied and Environmental Biology, 2017,23(2):343-350. (in Chinese) |
[1] | 杨静,张贺,李双霜,李桂花,张建峰. 砂质潮土施用改良剂对土壤动物群落特征的影响[J]. 中国农业科学, 2022, 55(16): 3185-3199. |
[2] | 张晨曦, 田明慧, 杨硕, 杜嘉琪, 何堂庆, 仇云鹏, 张学林. 酸性土壤中丛枝菌根真菌菌剂多样性对玉米产量及其磷钾吸收的影响[J]. 中国农业科学, 2022, 55(15): 2899-2910. |
[3] | 龚小雅,石记博,方凌,方亚鹏,吴凤芝. 淹水对辣椒连作土壤化学性质与微生物群落结构的影响[J]. 中国农业科学, 2022, 55(12): 2472-2484. |
[4] | 张学林,何堂庆,张晨曦,田明慧,李晓立,吴梅,周亚男,郝晓峰. 丛枝菌根真菌对玉米生育期土壤N2O排放的影响[J]. 中国农业科学, 2022, 55(10): 2000-2012. |
[5] | 刘彦伶,李渝,张艳,张雅蓉,黄兴成,张萌,张文安,蒋太明. 长期施用磷肥和有机肥黄壤微生物量磷特征[J]. 中国农业科学, 2021, 54(6): 1188-1198. |
[6] | 邵美琪,赵卫松,苏振贺,董丽红,郭庆港,马平. 盐胁迫下枯草芽孢杆菌NCD-2对番茄促生作用及对土壤微生物群落结构的影响[J]. 中国农业科学, 2021, 54(21): 4573-4584. |
[7] | 任嘉欣,刘京,陈轩敬,张跃强,张勇,王洁,石孝均. 长期施肥紫色土有效磷变化及其对稻麦轮作产量的影响[J]. 中国农业科学, 2021, 54(21): 4601-4610. |
[8] | 赵卫松,郭庆港,苏振贺,王培培,董丽红,胡卿,鹿秀云,张晓云,李社增,马平. 马铃薯健株与黄萎病株根际土壤真菌群落结构及其对碳源利用特征[J]. 中国农业科学, 2021, 54(2): 296-309. |
[9] | 孔亚丽,朱春权,曹小闯,朱练峰,金千瑜,洪小智,张均华. 土壤微生物介导植物抗盐性机理的研究进展[J]. 中国农业科学, 2021, 54(10): 2073-2083. |
[10] | 刘凯,刘佳,陈晓芬,李委涛,江春玉,吴萌,樊剑波,李忠佩,刘明. 长期施用磷肥水稻土微生物量磷的季节变化特征与差异[J]. 中国农业科学, 2020, 53(7): 1411-1418. |
[11] | 李小磊,张玉军,申凤敏,姜桂英,刘芳,柳开楼,刘世亮. 长期施肥对红壤性水稻土不同土层活性有机质及碳库管理指数的影响[J]. 中国农业科学, 2020, 53(6): 1189-1201. |
[12] | 张秀芝,李强,高洪军,彭畅,朱平,高强. 长期施肥对黑土水稳性团聚体稳定性及有机碳分布的影响[J]. 中国农业科学, 2020, 53(6): 1214-1223. |
[13] | 李亚林,张旭博,任凤玲,孙楠,徐梦,徐明岗. 长期施肥对中国农田土壤溶解性有机碳氮含量影响的整合分析[J]. 中国农业科学, 2020, 53(6): 1224-1233. |
[14] | 赵卫松,郭庆港,李社增,王培培,鹿秀云,苏振贺,张晓云,马平. 花铃期棉花黄萎病抗病与感病品种对 土壤细菌群落结构的影响[J]. 中国农业科学, 2020, 53(5): 942-954. |
[15] | 王乐,陈延华,张淑香,马常宝,孙楠,李春花. 长期施肥下华北潮土生产力演变及影响因素分析[J]. 中国农业科学, 2020, 53(11): 2232-2240. |
|