外种猪SLA-MS分型与免疫参数的关联

doi:10.3864/j.issn.0578-1752.2012.05.020

摘要/Abstract

摘要： 【目的】研究大白猪、长白猪的白细胞抗原复合体（swine leukocyte antigen，SLA）区域微卫星遗传多样性，探讨单倍型与免疫参数的相关性，为猪抗病育种提供参考依据。【方法】在SLA区域内筛选了13个多态性丰富的微卫星座位计算遗传多样性，并在Ⅰ、Ⅱ类区域筛选靠近功能基因且多态性丰富的微卫星进行单倍型分型，分析单倍型与免疫参数的关联。【结果】大白猪的期望杂合度（ =0.70）和多态信息含量（ =0.65）均分别高于长白猪（ =0.67， =0.62）；Ⅰ类区域6种单倍型与免疫参数的多重比较表明，H2的红细胞平均血红蛋白浓度（mean corpuscular hemoglobin concentration，MCHC）分别显著高于H3和H5的值（P＜0.05，P＜0.01）；H2的血小板分布宽度（platelet distribution width，PDW）显著低于H3的值（P＜0.05）。Ⅱ类区域的12种单倍型与免疫参数的多重比较表明，H1′的红细胞计数（red blood cell count，RBC）差异显著低于H6′、H9′对应的值（P＜0.05）；H1′的淋巴细胞（lymphocytes，LYM）百分比显著低于单倍型H7′的值（P＜0.05），极显著低于H6′、H9′和H11′的值（P＜0.01）；H6′的MCHC的数值显著低于H7′的值（P＜0.05），极显著低于H1′、H11′的值（P＜0.01）；H9′的MCHC的值显著低于H1′、H7′和H11′的值（P＜0.05）；H9′的红细胞平均体积（mean corpuscular volume, MCV）显著低于单倍型H1′、H6′和H11′的值（P＜0.05），极显著低于H7′的值（P＜0.01）；H11′的单核细胞（monocytes，MONO）百分比显著低于H6′、H7′（P＜0.05）和H1′的值（P＜0.01），H6′、H7′和H9′的值分别显著低于H1′的值（P＜0.05，P＜0.01）。【结论】大白猪的SLA微卫星遗传多样性高于长白猪，SLA区域不同微卫星单倍型与免疫参数PDW、MCHC、RBC、MCV、LYM、MONO存在关联，这可进一步用于猪的健康指数的评价。

关键词: 猪白细胞抗原复合体, 微卫星分型, 单倍型, 主成分分析, 免疫参数

Abstract: 【Objective】 The polymorphisms and the haplotypes of the swine leukocyte antigen complex (SLA) were investigated in Landrace and Yorkshire pigs using microsatellites (MS). The association between haplotypes and immune parameters was analyzed in order to provide references for disease resistance breeding in pigs.【Method】The genetic diversity parameters were calculated by thirteen microsatellites, which were in SLA region, the haplotypes in class Ⅰ and class Ⅱ were typed by some selected microsatellites typing, and the association between haplotypes and immune parameters were studied by microsatellites in class Ⅰand class Ⅱ regions.【Result】The expected heterozygosity ( =0.70) and polymorphism information content ( =0.65) of Yorkshire pigs were higher than those（ =0.67， =0.62）of Landrace pigs, respectively. The multiple comparison results of six haplotypes and immune parameters in SLA Ⅰ showed that the mean corpuscular hemoglobin concentration (MCHC) of H2 was higher than that of H3 (P＜0.05) and H5 (P＜0.01), respectively. The platelet distribution width (PDW) of H2 was lower than that of H3 (P＜0.05). The results of multiple comparisons between twelve haplotypes and immune parameters in SLAⅡ showed that the mean of red blood cell count (RBC) of H1′ was lower than that of H6′ and H9′ (P＜0.05), respectively. The value of lymphocytes ratio (LYM) of H1′ was lower than that of H7′ (P＜0.05), and extremely lower than that of H6′, H9′ and H11′ (P＜0.01), respectively. The mean of MCHC of H6′was lower than that of H7′ (P＜0.05), H1′ and H11′ (P＜0.01), while the value of MCHC of H9′ was lower than that of H1′, H7′ and H11′ (P＜0.05), respectively. The mean corpuscular volume (MCV) of H9′ was lower than that of H1′, H6′ and H11′ (P＜0.05) and extremely lower than that of H7′ (P＜0.01), respectively. The value of monocytes ratio (MONO) of H11′ was significantly lower than that of H6′, H7′ (P＜0.05) and H1′ (P＜0.01), while the value of H6′, H7′and H9′ was lower than that of H1′ (P＜0.05, P＜0.01), respectively.【Conclusion】The polymorphisms of Yorkshire pigs were higher than those of Landrace pigs, and the associations were detected between some haplotypes of SLA-MS and immune parameters, which were PDW, MCHC, RBC, MCV, LYM and MONO. These results could be used in evaluation of health index in future.

Key words: SLA, microsatellite typing, haplotypes, principal component analysis, immune parameters

于辉, 刘荣辉, 左启祯, 李岩, 李华. 外种猪SLA-MS分型与免疫参数的关联[J]. 中国农业科学, 2012, 45(5): 981-989.

YU Hui, LIU Rong-Hui, ZUO Qi-Zhen, LI Yan, LI Hua. The Association Between the SLA Microsatellites Typing and the Immune Parameters of Introduced Pig Breeds[J]. Scientia Agricultura Sinica, 2012, 45(5): 981-989.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2012.05.020

https://www.chinaagrisci.com/CN/Y2012/V45/I5/981

参考文献

[1]于辉, 李彬, 李华, 颜其贵. SLA与免疫抗病的研究进展. 中国畜牧杂志, 2009, 45(5): 60-64.

Yu H, Li B, Li H, Yan Q G. Advance in swine leukocyte antigen associated with immunity and disease resistance. Chinese Journal of Animal Science, 2009, 45(5): 60-64. (in Chinese)

[2]袁树楷, 王金勇, 谢和芳, 李琴, 白小青. 猪抗病育种候选基因研究进展. 中国畜牧杂志, 2007, 43(15): 50-52.

Yuan S K, Wang J Y, Xie H F, Li Q, Bai X Q. Advances in candidate genes of porcine disease resistance. Chinese Journal of Animal Science, 2007, 43(15): 50-52. (in Chinese)

[3]Lunney J K, Chen H B. Genetic control of host resistance to porcine reproductive and respiratory syndrome virus (PRRSV) infection. Virus Research, 2010, 154(1/2): 161-169.

[4]Lunney J K. Advances in swine biomedical model genomics. International Journal of Biological Sciences, 2007, 3(3): 179-184.

[5]Wimmers K, Schellander K, Ponsuksili S. BF, HP, DQB and DRB are associated with haemolytic complement activity, acute phase protein reaction and antibody response in the pig. Veterinary Immunology and Immunopathology, 2004, 99(3/4): 215-228.

[6]Lumsden J S, Kennedy B W, Mallard B A, Wilkie B N. The influence of the swine major histocompatibility genes on antibody and cell-mediated immune responses to immunization with an aromatic- dependent mutant of Salmonella typhimurium. Canadian Journal of Veterinary Research, 1993, 57: 14-18.

[7]Gerner W, Denyer M S, Takamatsu H H, Wileman T E, Wiesmüller K H, Pfaff E, Saalmüller A. Identification of novel foot-and-mouth disease virus specific T-cell epitopes in c/c and d/d haplotype miniature swine. Virus Research, 2006, 121(2): 223-228.

[8]Chung H, McClure M C. Characterization of microsatellite loci in the SLA class I region. Genomics, 2011, 97(4): 223-234.

[9]Tanaka M, Ando A, Renard C, Chardon P, Domukai M, Okumura N, Awata T, Uenishi H. Development of dense microsatellite markers in the entire SLA region and evaluation of their polymorphisms in porcine breeds. Immunogenetics, 2005, 57(9): 690-696.

[10]Ho C S, Lunney J K, Franzo-Romain M H, Martens G W, Lee Y J, Lee J H, Wysocki M, Rowland R R, Smith D M. Molecular characterization of swine leucocyte antigen class I genes in outbred pig populations. Animal Genetics, 2009, 40(4): 468-478.

[11]Ho C S, Franzo-Romain M H, Lee Y J, Lee J H, Smith D M. Sequence-based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines. International Journal of Immunogenetics, 2009, 36(4): 231-234.

[12]Ho C S, Martens G W, Amoss M S, Jr., Gomez-Raya L, Beattie C W, Smith D M. Swine leukocyte antigen (SLA) diversity in Sinclair and Hanford swine. Developmental and Comparative Immunology, 2010, 34(3): 250-257.

[13]Ho C S, Lunney J K, Lee J H, Franzo-Romain M H, Martens G W, Rowland R R, Smith D M. Molecular characterization of swine leucocyte antigen class II genes in outbred pig populations. Animal Genetics, 2010, 41(4): 428-432.

[14]Ando A, Uenishi H, Kawata H, Tanaka-Matsuda M, Shigenari A, Flori L, Chardon P, Lunney J K, Kulski J K, Inoko H. Microsatellite diversity and crossover regions within homozygous and heterozygous SLA haplotypes of different pig breeds. Immunogenetics, 2008, 60(7): 399-407.

[15]Yeom S C, Park C G, Lee B C, Lee W J. SLA typing using the PCR-SSP method and establishment of the SLA homozygote line in pedigreed SNU miniature pigs. Animal Science Journal, 2010, 81(2): 158-164.

[16]Soe O K, Ohba Y, Imaeda N, Nishii N, Takasu M, Yoshioka G, Kawata H, Shigenari A, Uenishi H, Inoko H, Ando A, Kitagawa H. Assignment of the SLA alleles and reproductive potential of selective breeding Duroc pig lines. Xenotransplantation, 2008, 15(6): 390-397.

[17]Gao Y, Flori L, Lecardonnel J, Esquerré D, Hu Z L, Teillaud A, Lemonnier G, Lefèvre F, Oswald I P, Rogel-Gaillard C. Transcriptome analysis of porcine PBMCs after in vitro stimulation by LPS or PMA/ionomycin using an expression array targeting the pig immune response. BMC Genomics, 2010, 11(292): 1-25.

[18]温立斌, 何孔旺, 杨汉春, 倪艳秀, 张雪寒, 俞正玉, 茅爱华. 类猪圆环病毒因子 P1 感染对猪红细胞的影响. 内蒙古农业科技, 2009, 2: 46-48.

Wen L B, He K W, Yang H C, Ni Y X, Zhang X H, Yu Z Y, Mao A H. Effect of porcine circovirus type 2-like agents p1 infection on porcine erythrocytes. Inner Mongolia Agricultural Science and Technology, 2009, 2: 46-48. (in Chinese)

[19]李洪涛, 顾为望, 袁进, 吴清洪, 王万山, 林继红, 王纯耀. 实验用西藏小型猪原代和第一代间部分血液指标比较. 郑州大学学报: 医学版, 2008, 43(1): 63-65.

Li H T, Gu W W, Yuan J, Wu Q H, Wang W S, Lin J H, Wang C Y. A comparative medical study on some haemal parameters of the experimental Tibet mini-pigs, Journal of Zhengzhou University: Medical Sciences, 2008, 43(1): 63-65. (in Chinese)

[20]包文斌, 叶兰, 潘章源, 朱璟, 黄雪根, 华金第, 吴圣龙. 苏太猪FUT1基因M307位点多态性及其对部分免疫指标的遗传效应分析. 畜牧兽医学报, 2010, 41(10): 1219-1224.

Bao W B, Ye L, Pan Z Y, Zhu J, Huang X G, Hua J D, Wu S L. Polymorphism and genetic effect of FUT1 gene M307 on some immune parameters in Sutai pigs. Chinese Journal of Animal and Veterinary Sciences, 2010, 41(10): 1219-1224. (in Chinese)

[21]Nuñez Y, Ponz F, Gallego F J. Microsatellite-based genotyping of the swine lymphocyte alloantigens (SLA) in miniature pigs. Research in Veterinary Science, 2004, 77(1): 59-62.

[22]Ding X D, Simianer H, Zhang Q. A new method for haplotype inference including full-sib information. Genetics, 2007, 177(3): 1929-1940.

[23]Smith T P, Rohrer G A, Alexander L J, Troyer D L, Kirby-Dobbels K R, Janzen M A, Cornwell D L, Louis C F, Schook L B, Beattie C W. Directed integration of the physical and genetic linkage maps of swine chromosome 7 reveals that the SLA spans the centromere. Genome Research, 1995, 5(3): 259-271.

[24]Lunney J K, Ho C S, Wysocki M, Smith D M. Molecular genetics of the swine major histocompatibility complex, the SLA complex. Developmental and Comparative Immunology, 2009, 33(3): 362-374.

[25]Ho C S, Lunney J K, Ando A, Rogel-Gaillard C, Lee J H, Schook L B, Smith D M. Nomenclature for factors of the SLA system, update 2008. Tissue Antigens, 2009, 73(4): 307-315.

[26]李洪涛, 顾为望, 袁进, 吴清洪, 王万山, 林继红, 杨海英, 赵乐, 曾昭志. 亚热带气候环境下西藏小型猪部分血液生理生化指标的比较. 中国实验动物学报, 2006, 14(4): 311-314.

Li H T, Gu W W, Yuan J, Wu Q H, Wang W S, Lin J H, Yang H Y, Zhao Y, Zeng Z Z, Comparison of some blood physiological and biochemical parameters in Tibet mini-pigs raised in subtropical environment. Acta Laboratorium Animalis Scientia Sinica, 2006, 14(4): 311-314. (In chinese)

[27]刘江伟, 张永久, 李泽信, 许永华, 龙小平, 赵笑云, 李广生, 常德从. 长白仔猪血常规及生化指标正常值探讨. 中国比较医学杂志, 2007, 17(7): 393-394.

Liu J W, Zhang Y J, Li Z X, Xu Y H, Long X P, Zhao X Y, Li G S. Chang D C. Investigation on the normal values of blood routine and serum biochemistry in Chang-Bai piglet. Chinese Journal of Comparative Medicine, 2007, 17(7): 393-394. (in Chinese)