关键词: 丛枝菌根, 黄瓜, 低温胁迫, 抗氧化酶

Abstract: 【objective】The aim of this experiment was to study the effects of Arbuscular mycorrhiz fungi(AMF) on the changes in growth and physiological in cucumber (Cucumis sativus L.) seedlings.【Method】Cucumber variety ‘Jinchun No.2’ was subjected to low temperature treatment of 15/10℃ at day and Night in the artificial climate chamber. The effects of AMF on the growth and the changes in root activity, chlorophyll, soluble protein and malondialdehyde (MDA) contents, superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) activities under low temperature stress in the seedling leaves were investigated.【Result】Under low temperature stress, the increment of height, stem diameter, fresh weight, dry weight and root-shoot ratio decreased, chlorophyll contents, root activity and SOD ,CAT activities decreased, MDA, soluble protein contents and POD activities increased in cucumber seedlings leaves. AMF-inoculation significantly promoted seedlings growth and chlorophyll, soluble protein contents, POD activities, while reduced MDA contents and SOD,CAT activities.【Conclusion】The results obtained from this experiment showed that under low temperature stress AMF could promote the accumulation of proline, raise the activities of antioxidant enzymes, decrease the membrane lipid peroxidation and improve the adaptability of cucumber seedlings.

Key words: Arbuscular mycorrhiza, Cucumber, Low temperture stress, Antioxidant enzyme