关键词: 水稻, 剑叶, 基因芯片, 光合相关基因, 叶绿素含量, 光合速率

Abstract:

【Objective】 The response of photosynthesis-related genes to low nitrogen stress were researched, thus providing a molecular basis for improving the absorption efficiency and utilization efficiency of nitrogen fertilizer in rice. 【Method】 The Agilent rice genome arrays were used to study the expression change of photosynthesis-related genes in two rice cultivars with different chlorophyll contents under low nitrogen fertilizer stress. 【Result】 The results showed that chlorophyll content and photosynthetic rate of the flag leaf were decreased under low nitrogen stress in SN19-6 and Fengjin, but the degree of decrease was significantly different between SN19-6 and Fengjin. A total of 41 photosynthesis-related genes (14 photosynthesis-related genes were down-regulated and 27 photosynthesis-related were up-regulated at the transcription level) in flag leaf of supper-green rice SN19-6 and 29 photosynthesis-related genes (15 photosynthesis-related genes were down-regulated and 14 photosynthesis-related genes were up-regulated at the transcription level) in flag leaves of Fengjin were affected by low nitrogen stress. The photosynthesis-related genes for expressed cultivar special-response to low nitrogen stress, with 29 photosynthesis-related genes for SN19-6 and 17 for Fengjin. There was an overlap of photosynthesis-related genes in response to low nitrogen stress between SN19-6 and Fengjin, with 5 up-regulated and 7 down-regulated at the transcription level, respectively.【Conclusion】 Expression of photosynthesis-related genes were affected by low nitrogen stress in flag leaf, and there are genes of cultivar special-response as well as overlap between SN19-6 and Fengjin.

Key words: rice (Oryza sativa L.), flag leaf, microarray, photosynthesis-related genes, chlorophyll content, photosynthetic rate