关键词: 油菜, 遗传图谱, AFLP, SRAP, SSR

Abstract: A genetic linkage map of Brassica napus was constructed using 143 SSR, 118 AFLP, and 137 SRAP markers based on an F2 population of 184 individuals, which was developed by crossing “SI-1300” and “Eagle”. These markers were aligned in 21 linkage groups (LGs), and the map covered 1949.8 cM with an average marker interval of 4.9 cM. The public microsatellites (SSR markers) contributed to establish the links between the map of Parkin et al. and ours. Our results showed that most of LGs were aligned with the standard map except LG 20 and LG 21，and the distribution of markers on all LGs were uniform except N7 based on the analysis of Kolmogorov-Smirnov. However, the distorted markers were mainly cluster in LGs, and eight segregation distortion regions on seven LGs were detected. In addition, the results showed that SRAP markers may be a better type of markers than AFLP in map construction. In conclusion, according to the analysis of the marker distribution on LG, the map coverage and the marker density, the linkage map constructed in this study was of enough quality .Threrefore, this map will be a fine platform for the location of QTLs, studies on heterosis, and using the information of others.

Key words: B. napus, genetic map, AFLP, SRAP, SSR