中国农业科学 ›› 2007, Vol. 40 ›› Issue (3): 633-637 .

• 研究简报 • 上一篇    下一篇

丙烷脒对灰霉病菌菌丝形态和超微结构的影响

陈安良,冯美杰,冯俊涛,何 军,张 兴   

  1. 西北农林科技大学无公害农药研究服务中心
  • 收稿日期:2005-03-10 修回日期:1900-01-01 出版日期:2007-03-10 发布日期:2007-03-10

Effects of Propamidine on Mycelium Shape and Ultrastructure Changes of Botrytis cirerea

  1. 西北农林科技大学无公害农药研究服务中心
  • Received:2005-03-10 Revised:1900-01-01 Online:2007-03-10 Published:2007-03-10

摘要: 【目的】杀菌剂对病菌菌丝形态和超微结构的影响,是进行杀菌剂作用机理研究的基础。【方法】采用扫描电镜和透射电镜,研究了丙烷脒对灰霉病菌菌丝形态和超微结构的影响。【结果】药剂处理1 d后,菌丝局部不规则膨胀,菌丝隔膜间距变短;药剂处理3 d,菌丝局部严重膨大,且膨胀部位失水畸形。透射电镜观察表明,药剂处理1 d后,菌丝细胞线粒体体积增大、数目增多,细胞核的结构已不明显,细胞内出现囊泡;3 d后,各细胞器结构紊乱,并含有大量的无膜透明内含物,有较大的空腔形成,细胞壁外围沉积大量的外渗物,菌丝组织坏死。【结论】丙烷脒对灰霉病菌菌丝形态和超微结构的影响不同于常用杀菌剂品种,预示丙烷脒的作用机理独特。对丙烷脒作用机理进行深入研究,有望发现杀菌剂新的作用靶标,为杀菌剂新品种的开发提供依据。

关键词: 丙烷脒, 灰霉病菌, 超微结构

Abstract: The mycelium ultrastructure changes of Botrytis cirerea treated with propamidine was studied through light microscopy (LM) and transmission electro microscopy (TEM). Treated with propamidine one day, by LM, the space between mycelium septum was shortened, the part of mycelium was swelled; After three days, the swelled part of mycelium was malformation. Through TEM, the volume of mycelium cell mitochondrion was swollen , its number increased after treated with propamidine one day; Then treated three days, the configuration of mycelium cell was destroyed. Above cytological changes result in the necrosis of hyphal cell.

Key words: Propamidine, Botrytis cirerea, Ultrastructure