中国农业科学 ›› 2016, Vol. 49 ›› Issue (2): 251-259.doi: 10.3864/j.issn.0578-1752.2016.02.006

• 耕作栽培·生理生化·农业信息技术 • 上一篇    下一篇

外源一氧化氮对干旱胁迫下玉米幼苗光合作用的影响

邵瑞鑫1, 2,李蕾蕾1,郑会芳1,信龙飞1,苏小雨1,冉午玲1,杨青华1   

  1. 1河南农业大学农学院/省部共建小麦玉米作物学国家重点实验室,郑州 450002
    2中国农业科学院农田灌溉研究所/农业部作物需水与调控重点实验室,河南新乡 453002
  • 收稿日期:2015-05-11 出版日期:2016-01-16 发布日期:2016-01-16
  • 通讯作者: 杨青华,E-mail:yangqh2000@163.com
  • 作者简介:邵瑞鑫,E-mail:shao_rui_xin@126.com
  • 基金资助:
    教育部博士点新教师类联合基金(20124105120008)、农业部作物需水与调控重点实验室开放课题(CWRR201404)、小麦玉米作物学国家重点实验室(SKL2014ZH-11)

Effects of Exogenous Nitric Oxide on Photosynthesis of Maize Seedlings Under Drought Stress

SHAO Rui-xin1, 2, LI Lei-lei1, ZHENG Hui-fang1, XIN Long-fei1, SU Xiao-yu1, RAN Wu-ling1, YANG Qing-hua1   

  1. 1Agronomy College of Henan Agriculture University/State Key Laboratory of Wheat and Maize Crop Science, Zhengzhou 450002
    2Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Crop Water Use and Regulation, Ministry of Agriculture, Xinxiang 453002, Henan
  • Received:2015-05-11 Online:2016-01-16 Published:2016-01-16

摘要: 【目的】探明干旱胁迫下一氧化氮(NO)对玉米幼苗光合作用的调节作用。【方法】以驻玉309为试验材料,在光照培养箱内采用水培的方法,研究了外源NO供体SNP预处理3 d后,进行20% PEG-6000(-0.8 MPa)模拟干旱胁迫3 d后玉米幼苗的生长参数、叶绿体超微结构、叶片气体交换参数、叶绿素荧光参数和D1蛋白表达的变化。【结果】干旱胁迫条件下玉米幼苗的光合速率(Pn)、气孔导度(Gs)及光系统II(PSII)最大光化学效率(Fv/Fm)、PSII潜在活性(Fv/Fo)分别比对照下降了68.4%、87.2%、15.8%、31.3%;而J点的相对可变荧光(Vj)和单位反中心耗散能量(DIo /RC)则分别上升了20.9%和21.2%;此外,叶片的叶绿体结构明显遭到破坏,D1蛋白的含量显著降低。但是,与干旱胁迫处理相比,经过SNP预处理后进行干旱胁迫处理的玉米幼苗Pn、Gs、Fv/Fm、Fv/Fo明显增加56.6%、202.5%、15.8%、30.7%,而Vj、DIo/RC下降了22.7%和25.4%;叶绿体的类囊体片层恢复整齐,基粒片层变清晰;D1蛋白的相对含量明显增加了94.7%;以上这些变化与干旱条件下SNP预处理后NO含量的增加有关。【结论】在干旱条件下,NO可能参与调节了PSII反应中心D1蛋白的表达并稳定PSII反应中心的结构和功能,以改善玉米幼苗的光合作用和生长发育,从而提高玉米幼苗对干旱胁迫的适应性。

关键词: 玉米, 干旱胁迫, 一氧化氮, D1蛋白, 光合作用, 叶绿体超微结构

Abstract: 【Objective】To evaluate the effects of exogenous nitric oxide on the photosynthesis of maize (Zea mays L.) under drought stress. 【Method】The hydroponic experiment was conducted in a growth chamber with the Zhuyu 309 as the material. After being pretreated with sodium nitroprusside (SNP) for three days, these materials were simulated drought stress with 20% PEG-6000 for another three days. The changes of maize seedling’s growth, gas exchange, and chlorophyll fluorescence parameters, chloroplast ultrastructure and expression of D1 protein were investigated; 【Result】The photosynthetic rate (Pn), stomatal conductance (Gs), PSII maximum photochemical efficiency (Fv/Fm), PSII potential activity (Fv/Fo) were decreased by 68.4%, 87.2%, 15.8%, 31.3%,respectively under drought stress; The parameters of relative variable fluorescence intensity at J-step (Vj) and dissipated energy flux per reaction center (DIo/RC) increased by 20.9% and 21.2%. The ultrastructure of chloroplast in the leaf was destroyed and the structure of stacked grana was disintegrated. Moreover, the osmiophilic grains increased and the chloroplast was slowly divorced from the cell wall due to the drought. The content of D1 protein decreased significantly with Western-blotting analysis. However, with the treatment of SNP and then drought stress, the Pn, Gs, Fv/Fm, Fv/Fo were increased by 56.6 %, 202.5 %, 15.8 %, 30.7 % and the Vj decreased by 22.7% and 25.4% compared with the drought stress. The thylakoid lamellae arranged regularly and the grana were clear; and the relative amounts of D1 protein increased by 94.7%. The above changes were related to the increased NO content after SNP pretreatment under drought condition.【Conclusion】These results suggested that NO is involved in regulating the D1 protein expression and stabilizing the structure and function of PSII reaction centers to improve photosynthesis and growth of maize seedlings, which improved the adaptability of maize seedlings to drought stress. 

Key words: maize, drought stress, nitric oxide, D1 protein, photosynthesis, chloroplast ultrastructure