中国农业科学 ›› 2007, Vol. 40 ›› Issue (3): 594-600 .

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

IGF-Ⅰ对不同绵羊皮肤中5个毛囊相关生长因子基因表达的影响

李 岩,廖和荣,李知勉,孙 杰,李大全   

  1. 新疆石河子大学动物科技学院
  • 收稿日期:2005-11-17 修回日期:1900-01-01 出版日期:2007-03-10 发布日期:2007-03-10
  • 通讯作者: 廖和荣

Effect of IGF-1 on expression of related hair follicle growth factors and receptors in varied sheepskin

  

  1. 新疆石河子大学动物科技学院
  • Received:2005-11-17 Revised:1900-01-01 Online:2007-03-10 Published:2007-03-10

摘要: 【目的】为探讨外源性IGF-Ⅰ对绵羊皮肤中毛囊生长调控的影响。【方法】选取36只新疆细毛羊,随机分成6组。在每只绵羊的左、右两侧肩胛前部分别进行拔毛、剃毛处理。同时在左侧肩胛前部的拔毛、剃毛和正常区的交叉处注射0.5ml IGF-Ⅰ(10ng•ml-1),然后在第0、3、6、9、12、50天采取皮样。用RT-PCR方法,测定各皮肤中GHR、IGF-Ⅰ、IGF-ⅠR、KAP3.2和KAP6-1的mRNA相对丰度。【结果】(1)IGF-Ⅰ可以下调正常皮肤中GHR基因的表达,对IGF-Ⅰ、IGF-ⅠR的表达没有显著的影响,能显著提高KAP3.2和KAP6-1的转录水平;(2)IGF-Ⅰ在3 d内可降低拔毛皮肤中GHR的表达,6 d以上无明显影响;对剃毛皮肤中的GHR表达无显著影响。IGF-Ⅰ对拔毛皮肤中的IGF-Ⅰ和IGF-ⅠR的表达均无明显影响;对剃毛皮肤中IGF-Ⅰ的表达有明显的促进作用,但IGF-ⅠR的变化不明显。IGF-Ⅰ对拔毛皮肤和剃毛皮肤中的KAP3.2 和KAP6-1的表达都能显著提高,且表达模式也基本一致,KAP3.2的表达早于KAP6-1的表达。【结论】IGF-Ⅰ对绵羊皮肤中的GHR和IGF-ⅠR的表达均无显著影响,对KAP3.2和KAP6-1的表达能显著提高,但呈现出颠倒的特定时序性,这是否是外源性IGF-Ⅰ不能促进体内毛囊生长的原因所在,有待进一步研究。

关键词: 绵羊, 皮肤, 拔毛剃毛, IGF-Ⅰ, 基因表达

Abstract: Thirty-six healthy and similar Xin jiang sheep were selected and divided into six groups at random. Each animal was plucked and shaved at pro-scapulae region and injected intradermally with 0.5 mL IGF-1 (10ng/mL) at the cross section of plucking, shaving and control, except sheep, as control of no injection IGF-1, sampled for 0 day. When sampled, the treatment sheep were selected one out of five groups at random, and sampled plucked tissues, shaved tissues and control, respectively for 3,6,9,12 and 50 days. The expression of encoding growth hormone receptor (GHR), insulin-like growth factor1 (IGF-1), insulin-like growth factor receptor (IGF-1R), KAP3.2 and KAP6-1 mRNA were measured in each sample, with relative quantitative reverse transcription polymerase chain reaction (RT-PCR) using beta-actin as an internal standard. The results indicate that, 1). In normal sheepskin, the expression of GHR in injected IGF-1 decreased, no evidently changes of IGF-1 and IGF-1R, and that of KAP3.2 and KAP6-1 increased markedly.2). In plucked sheepskin, the abundance of GHR mRNA in injected IGF-1 reduced in short term (3 day), but no clearly changes in long term (above 6 day); meanwhile, compared with control, there were no changes of expression of IGF-1 and IGF-1R. The pattern of expression changes of KAP3.2 and KAP6-1 were similar, which were promoted significantly in both, but the expression of KAP3.2 prior to that of KAP6-1.3). In shaved sheepskin, the levels of GHR and IGF-1 mRNA were increased dramatically after injection IGF-1, but there has no clear fluctuation of IGF-1R; likely, the pattern of expression changes of KAP3.2 and KAP6-1 were similar, which were promoted significantly in both, but the expression of KAP3.2 prior to that of KAP6-1, too.

Key words: sheepskin, pluck and shave, IGF-1, gene expression,