中国农业科学 ›› 2007, Vol. 40 ›› Issue (10): 2128-2135 .

• 作物遗传育种·种质资源 • 上一篇    下一篇

利用CSSL群体研究稻米加工品质相关QTL表达的稳定性

翁建峰,万向元 ,郭 涛,江 玲,翟虎渠,万建民   

  1. 南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心
  • 收稿日期:2006-07-04 修回日期:1900-01-01 出版日期:2007-10-10 发布日期:2007-10-10
  • 通讯作者: 万建民

Stable expression of QTL for milling quality of rice (oryza stativa L.) using CSSL population

  

  1. 南京农业大学作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心
  • Received:2006-07-04 Revised:1900-01-01 Online:2007-10-10 Published:2007-10-10

摘要: 【目的】研究稻米加工品质的分子遗传基础,为遗传改良和分子标记辅助选择提供有用信息。【方法】利用Asominori为遗传背景具有IR24染色体片段的置换系(CSSL)群体,在4个环境下对稻米糙米率、精米率和整精米率进行QTL定位和稳定性分析。【结果】结果共检测到30个QTL与稻米加工品质相关,其中qMR-6、qMR-8、qHR-3和qHR-6在4个环境中都能被重复检测到,影响精米率的qMR-6和qMR-8的平均贡献率分别为21.1%和22.2%;与整精米率相关的qHR-3和qHR-6,平均贡献率分别为34.6%和22.3%;且qMR-6、qMR-8、qHR-3和qHR-6对应的置换系与背景亲本Asominori在4个环境中相应性状的表现型之间都存在显著差异(P<0.05)。【结论】qMR-8、qBR-8b和qHR-8共定位在第8染色体R727-G1149区间的QTL簇上,在笔者以前的研究中发现该QTL簇包含与稻米蒸煮食味、外观和营养品质等相关的多个主效QTL,这为剖析同一染色体区段内的1个或多个基因协同调控多个品质性状形成的复杂代谢网络提供信息。

关键词: 水稻, 加工品质, 染色体片段置换系, QTL定位, 稳定性分析

Abstract: A chromosome segment substitution line (CSSL) population, derived from the cross of Asominori and IR24 with Asominori as the recurrent parent, was planted and phenotyped for brown rice rate (BR), milled rice rate (MR) and head rice rate (HR) in four sites. QTL for milling quality were characterized and expression stability of these QTL was further investigated by using target CSSLs harboring certain QTL. The phenotypic values of these three traits all showed continuous distributions and transgressive segregations in the CSSL population across the four environments.. Meanwhile, a total of 30 QTL were identified for the three quality traits, among which four QTL, qMR-6、qMR-8、qHR-3 and qHR-6, were all consistently detected in the four different environments. Moreover, phenotypic values were different significantly (P<0.05) between Asominori and the target CSSLs harboring any of the four QTL alleles, indicating expression stability of the four QTL was relatively higher. In addition, QTL qMR-8、qBR-8b and qHR-8 were co-located in the G1149-R727 interval on chromosome 8 carrying a QTL cluster. Interestingly, many major QTL affecting rice eating, cooking, appearance and nutrient quality was also mapped into the QTL cluster in our previous studies. These results thus obtained provide us a good chance to elucidate the complex mechanism that formation of many rice quality traits was simultaneously controlled by a gene or a gene cluster on chromosome 8.

Key words: Rice (oryza stativa L.), Milling quality, Chromosome Segment Substitution Line (CSSL), QTL mapping, expression stability