中国农业科学 ›› 2006, Vol. 39 ›› Issue (01): 210-214 .

• 研究简报 • 上一篇    下一篇

猪源马链球菌兽疫亚种中国分离株类M基因特性分析

范红结,陆承平,唐家琪   

  1. 南京农业大学农业部动物疫病诊断与免疫重点开放实验室
  • 收稿日期:2004-04-09 修回日期:1900-01-01 出版日期:2006-01-10 发布日期:2006-01-10
  • 通讯作者: 陆承平

Analysis of the M-Like Gene of Streptococcus zooepidemicus Chinese Isolates from Pig

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  1. 南京农业大学农业部动物疫病诊断与免疫重点开放实验室
  • Received:2004-04-09 Revised:1900-01-01 Online:2006-01-10 Published:2006-01-10

摘要: 【目的】阐明国内不同区域猪源马链球菌兽疫亚种分离株类M基因的特性,为疫苗研制提供指导。【方法】利用PCR技术,扩增国内9省市从1974~2003年分离的9株马链球菌兽疫亚种类M基因,并进行克隆、测序及序列分析。【结果】发现7株类M基因长度为1 137 bp,含一个阅读框,CG-74-63和CP-0104株的长度分别为1 143 bp及1 125 bp。除1株关节炎分离株CP-0104外,引致败血症的8株菌类M基因的核苷酸同源性高达95.4%~99.9%,推导的相应氨基酸序列同源性高达92.1%~100%。9株猪源菌与马源菌株及人源菌株类M基因的核苷酸同源性分别为81.6%~87.0%、81.7%~91.8%。9株猪源分离株类M蛋白信号肽、C末端细胞锚定区的序列均高度同源;除CP-0104外,其余8株高变区高度同源。【结论】国内不同区域猪源马链球菌兽疫亚种分离株类M基因同源性高。

关键词: 马链球菌兽疫亚种, 类M基因, 分析

Abstract: 【Objective】In order to clarify the features of the M-like gene of Streptococcus equi subsp. zooepidemicus isolates from Chinese pig for directing the selection of vaccine isolate.【Method】The M-like genes were amplified from 9 isolates of Streptococcus equi subsp. zooepidemicus from Chinese pig by PCR and the M-like gene of each isolate was cloned, sequenced and analyzed. 【Result】Nucleotide sequence analysis revealed one open reading frame in M-like protein gene of all isolates, with different length of 1 125 bp in CP-0104 isolate, 1 143 bp in CG-74-63 isolate, and 1 137 bp in the others. The nucleotide identities of M-like genes among 9 isolates except CP-0104 isolate were higher than 95.4%, while the identities between isolate CP-0104 and the remaining 8 strains were 87.1%-88.3%. W60 strain, an equine origin isolate, shared identities of 81.6%-87.0% with these 9 isolates from pig, and 25064 strain, a human origin isolate, shared identities of 81.7%-91.8% with these 9 isolates from pig. All the 9 isolates shared the common membrane anchor region and N-terminal signal peptide. 【Conclusion】The M-like gene of 9 isolates of Streptococcus equi subsp. zooepidemicus from Chinese pig share high identity.

Key words: Streptococcus equi subsp. zooepidemicus, M-like gene, Analysis