中国农业科学 ›› 2005, Vol. 38 ›› Issue (12): 2549-2554 .

• 研究简报 • 上一篇    下一篇

粳稻品种SSR多态性检出率的分析

鲍根良,富田桂,小林麻子,骆荣挺,张小明,王俊敏,叶胜海   

  1. 浙江省农业科学院
  • 收稿日期:2004-08-30 修回日期:2005-09-23 出版日期:2005-12-10 发布日期:2005-12-10
  • 通讯作者: 鲍根良

Analysis on SSR Polymorphisms of Japonica Rice Varieties

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  1. 浙江省农业科学院
  • Received:2004-08-30 Revised:2005-09-23 Online:2005-12-10 Published:2005-12-10

摘要: 用395对SSR引物测得多态性检出率:日本近缘品种(粳型)间为11.12%,日本近缘品种与意大利品种(粳型)间、与美国品种(籼型)间及与菲律宾品种(籼型)间分别为17.58%、20.80%和38.62%,显示相同粳稻生态型品种间多态性检出率低于不同生态型品种间,而相同粳稻生态型内,日本近缘品种间的多态性检出率更低。在日本近缘品种中,不同品种间多态性检出率差异较大,其变幅为3.79~15.95%。此外,不同染色体间引物的多态性检出率也存在较大差异,其中第10染色体的供试引物多态性检出率无论在不同生态型品种间或相同生态型品种间均最低。

关键词: 粳稻, 近缘品种, SSR分析, 多态性, 检出率

Abstract: 395 SSR (Simple sequence repeats) primer pairs were used to analyze 16 Japonica and Indica rice varieties. The result showed that the polymorphisms detected were 11.12% among Japanese closely related varieties (JCRV,Japonica), 17.58% between JCRV and Italy varieties (Japonica), 20.80% between JCRV and America varieties (Indica) and 38.62% between JCRV and Philippines varieties (Indica). It implied that polymorphisms were lower among identical Japonica type varieties than between different ecological type varieties, and lowest among JCRV in the identical Japonica type varieties. However, polymorphisms were obviously different among JCRV, variance ranges of that were from 3.79% to 15.95%. Furthermore, there were significant differences in polymorphisms among various chromosomes as well; the polymorphisms of SSR markers in tenth chromosomes were always lowest among any type varieties.

Key words: Japonica rice, Closely related variety, SSR analysis, Polymorphism, Detected rate