中国农业科学 ›› 2005, Vol. 38 ›› Issue (10): 2002-2006 .

• 植物保护 • 上一篇    下一篇

转Bt基因水稻生长期几种重要成分含量的变化研究

贾乾涛,石尚柏,杨长举,彭于发   

  1. 湖北省植物保护总站
  • 收稿日期:2005-01-18 修回日期:1900-01-01 出版日期:2005-10-10 发布日期:2005-10-10
  • 通讯作者: 石尚柏

Changes of Several Important Materials in Transgenic Bt Rice

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  1. 湖北省植物保护总站
  • Received:2005-01-18 Revised:1900-01-01 Online:2005-10-10 Published:2005-10-10

摘要: 对Bt水稻汕优63及其非转基因亲本汕优63不同生长期几种重要成分的含量进行了分析,以期为转Bt基因水稻的生态学和安全性评价提供基础代谢方面的依据。结果表明,在抽穗期,Bt水稻和非Bt水稻茎秆和叶片中大部分氨基酸的含量差异显著或极显著;在大部分生长期,Bt水稻和非Bt水稻氮、磷和硅的含量差异显著或极显著,但它们的变化趋势基本一致,氮和磷的含量在拔节期之后均呈下降趋势,而硅的含量则随水稻的生长发育呈上升趋势;在水稻生长前期,Bt水稻可溶性糖和还原糖的含量比非Bt水稻低,但在后期比非Bt水稻高,且差异均显著或极显著。

关键词: 转Bt基因水稻, 氨基酸, 氮, 磷, 硅, 可溶性糖, 还原糖

Abstract: In order to provide metabolism evidence for ecology and safety assessment of transgenic Bt rice, several important materials in transgenic Bt rice "Shanyou 63" with cry1Ab/cry1Ac genes and non-transgenic "Shanyou 63" without cry1Ab/cry1Ac genes were analyzed. Results showed that the contents of most amino acids in stems and leaves of transgenic Bt rice "Shanyou 63" and in non-transgenic rice "Shanyou 63" had significant or highly significant differences at heading stage. And during the majority growth stages, the contents of N, P and Si in two rice lines had significant or very significant difference, but their trends of change with time were basically accordant: The contents of N and P gradually decreased after stem-drawing stage, while the contents of Si gradually increased with the growth of rice. In the early growth stage, water-soluble sugar and reducing sugar contents in transgenic Bt rice "Shanyou 63" were significantly lower than those of non-transgenic "Shanyou 63", but in late growth stage, were significantly higher than those of non-transgenic "Shanyou 63".

Key words: Transgenic Bt rice, Amino acid, Nitrogen, Phosphor, Silicon, Water-solubility sugar, Reducing sugar