关键词: 海岛棉, 陆地棉, 花粉管通道导入, 种质, 遗传分析, SSR

Abstract: The genomic DNA of island cotton (Gossypium barbadense L. cv. Hai7124) was introduced into the upland cotton (G. hirsutum L. cv. Shiyuan321) by pollen tube pathway technique. New germplasm of high quality of fiber and a mutant with obviously change of morphology were obtained. Genetic analysis of the mutant indicated that the phenotype of the mutant was possibly controlled by a dominant allele that has homologous lethal effect. The result of SSR (simple sequence repeats) analysis showed that at least some exogenous DNA fragments were integrated into the genome of upland cotton Shiyuan321. It is deduced that some of the DNA fragments were integrated by homologous recombination mechanism.

Key words: Gossypium barbadense L., Gossypium hirsutum L., Pollen tube pathway, Germplasm, Genetic analysis, SSR