关键词: 普通小麦, Bcl, Rip, 遗传分析, 白粉病, 全蚀病

Abstract: Detection at molecular level and anti-disease identification of the T1, T2 and T3 transgenic wheats with Bcl and Rip genes indicated that segregation ratios of nptⅡ selective gene and functional gene Rip were consistent with Mendel's law，but functional gene Bcl was not in T1 generation. Southern blot showed that the alien genes had integrated into wheat genome and could be inherited to the following generations. The results also showed that there was just one copy of the alien genes in most T1 plants and most T2 homologous lines had two copies. After artificial inoculation of powdery mildew and wheat take-all, the transgenic plants demonstrated some resistance to them. And analysis of several agronomic traits indicated that transgenic plants had little difference in tiller, main spike length, spikelet number of main spike except plant height.

Key words: Common wheat, Bcl, Rip, Genetic analysis, Powdery mildew, Wheat take-all