关键词: 甘蔗, 蔗茅, F1子代, 基因组原位杂交, 核型

Abstract:

【Objective】The objective of current experiment was to study the chromosomal constitution and karyotype of F1 hybrids that derived from sexual cross between cultivated sugarcane (Saccharum spp.) and Erianthus fulvus.【Method】Both monocolor and bicolor GISH (Genomic in situ hybridization) were performed using digoxigenin-labeled E. fulvus DNA and biotin-labeled female parental DNA as probes, the karyotypes of 3 hybrids were analyzed based on the result of GISHs. 【Result】 The results of GISHs revealed the transmission of 7 to 10 E. fulvus chromosomes into progenies. The chromosomes of E. fulvus were broken because of the female parents prevented alien genetic materials from introgressing during distant hybridization and resulted in elimination and fragmentation of E. fulvus chromosomes. The GISH result on nuclear of progeny showed the chromatins came from male and female parent are not intermixed and stays at different regions. The karyotype formulas of 3 hybrids were 2n=92=80m (4SAT)+12sm. 2n=88=82m+6sm and 2n=88=78m(2SAT)+10sm,and their karyotypes were 2C, 2C and 2B, respectively. 【Conclusion】Chromosomal constitution of F1 hybrids that derived from sexual cross between cultivated sugarcane (Saccharum spp.) and E. fulvus were n+n and 2n+n. The results of karpotype analysis showed that the chromosomes of these three progenies are primitive.

Key words: Saccharum spp., Erianthus fulvus, F1 hybrids, GISH, karyotype