断根与打顶对番茄嫁接愈合的抑制作用

doi:10.3864/j.issn.0578-1752.2022.02.011

中国农业科学 ›› 2022, Vol. 55 ›› Issue (2): 365-377.doi: 10.3864/j.issn.0578-1752.2022.02.011

断根与打顶对番茄嫁接愈合的抑制作用

崔青青^1,²(),孟宪敏¹,段韫丹¹,庄团结¹,董春娟¹,高丽红²,尚庆茂¹()

¹中国农业科学院蔬菜花卉研究所/农业部园艺作物生物学与种质创制重点实验室,北京 100081
²中国农业大学园艺学院/设施蔬菜生长发育调控北京市重点实验室,北京 100193

收稿日期:2021-03-17 接受日期:2021-08-10 出版日期:2022-01-16 发布日期:2022-01-26
通讯作者: 尚庆茂
作者简介:崔青青,E-mail: cuiqq_caas@163.com。
基金资助:
“十三五”国家重点研发计划(2020YFD1000300);海南省重大科技计划(ZDKJ2021005);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-23-B10);中国农业科学院科技创新工程(CAAS- STIP-IVFCAAS)

Inhibiting Eeffect of Root-Cutting and Top-Pinching on Graft Healing of Tomato

CUI QingQing^1,²(),MENG XianMin¹,DUAN YunDan¹,ZHUANG TuanJie¹,DONG ChunJuan¹,GAO LiHong²,SHANG QingMao¹()

¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Beijing 100081
²College of horticulture, China Agricultural University/Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Beijing 100193

Received:2021-03-17 Accepted:2021-08-10 Online:2022-01-16 Published:2022-01-26
Contact: QingMao SHANG

摘要/Abstract

摘要：

【目的】断根嫁接苗和打顶双干或多干嫁接苗日益广泛用于番茄栽培,丰产作用显著。了解断根与打顶对番茄嫁接愈合的作用机制,可为番茄嫁接生产提供理论依据。【方法】以‘硬粉8号’为接穗品种,‘砧爱1号’为砧木品种,观测断根和打顶后番茄嫁接愈合进程、成活率和木质部连通性等,同时测定嫁接接合部生长素和细胞分裂素的含量及愈合相关基因的表达。【结果】断根和打顶抑制了嫁接接合部上方木质部分化基因VND7的表达,并显著延迟了木质部的重构。断根显著降低了嫁接后12和72 h接合部玉米素核苷（tZR）和反式玉米素（tZ）的含量,以及嫁接后12 h接合部吲哚-3-乙酸（IAA）,吲哚-3-乙酸甲酯（ME-IAA）和吲哚-3-甲醛（ICAld）的含量;打顶嫁接后12 h接合部IAA和ME-IAA含量显著低于对照和断根处理,下调了嫁接后3和12 h接合部上方和下方韧皮部分化相关基因NEN4表达水平。断根或打顶均下调了嫁接后3—48 h接合部上方细胞分裂相关基因Histone H4和SlcycB1-4的表达水平;断根和打顶同时处理使嫁接接合部IAA和ME-IAA含量显著低于断根处理,使tZR和tZ的含量及木质部连通性显著低于打顶处理。打顶和断根后分别在嫁接接合部施用10 mmol∙L^-1 IAA和10 mmol∙L^-1 6-BA可显著促进嫁接苗木质部的重构。【结论】断根和打顶分别降低接合部细胞分裂素和生长素积累,下调愈合相关基因表达,降低木质部输导能力,进而延缓番茄嫁接愈合进程。

关键词: 番茄, 嫁接, 打顶, 断根, 生长素, 细胞分裂素

Abstract:

【Objective】The root-cutting grafting seedlings and top-pinching double-stem grafting seedlings or multi-stem grafting seedlings have been widely used in tomato cultivation, which have significant effects on high yield. Understanding the mechanism of root-cutting and top-pinching on tomato graft healing could provide the theoretical basis for tomato grafting production. 【Method】Using ‘YingFen No. 8’ as scion and ‘ZhenAi No. 1’ as rootstock, the grafting healing process, survival rate and xylem connectivity of tomato after root-cutting and top-cutting were observed, and the contents of auxin and cytokinin as well as the expressions of healing related genes in the grafted part were measured. 【Result】Root-cutting and top-pinching inhibited the expression of xylem differentiation gene VND7 above the graft union, and significantly delayed xylem remodeling. The root-cutting treatment significantly decreased the contents of trans-Zeatin riboside (tZR) and trans-Zeatin (tZ) at 12 and 72 hours after grafting, as well as the contents of indole-3-acetic acid (IAA), methyl indole-3-acetate (ME-IAA) and indole-3-carboxaldehyde (ICAld) at 12 hours after grafting. The contents of IAA and ME-IAA at 12 hours after top-pinching grafting were significantly lower than those of the control and root-cutting treatment, and the expression level of the phloem differentiation related gene NEN4 at 3 and 12 hours after grafting was down-regulated. Root-cutting or top-pinching down-regulated the expression levels of cell division related genes Histone H4 and SlcycB1-4 above the graft union at 3-48 hours after grafting. Under root-cutting and top-pinching treatment, the contents of IAA and ME-IAA in grafting union were significantly lower than those under root-cutting treatment, and the contents of tZR and tZ and xylem connectivity were significantly lower than those under top-pinching treatment. The application of 10 mmol∙L ^-1 IAA and 10 mmol∙L^-1 6-BA at the grafted union after top-pinching and root-cutting could significantly promote the xylem remodeling of grafted seedlings. 【Conclusion】In conclusion, root-cutting and top-pinching could reduce the accumulation of cytokinin and auxin, down-regulate the expression of genes related to healing, reduce xylem transduction capacity, and delay the process of tomato grafting healing.

Key words: tomato, graft, top-pinching, root-cutting, auxin, cytokinin

崔青青, 孟宪敏, 段韫丹, 庄团结, 董春娟, 高丽红, 尚庆茂. 断根与打顶对番茄嫁接愈合的抑制作用[J]. 中国农业科学, 2022, 55(2): 365-377.

CUI QingQing, MENG XianMin, DUAN YunDan, ZHUANG TuanJie, DONG ChunJuan, GAO LiHong, SHANG QingMao. Inhibiting Eeffect of Root-Cutting and Top-Pinching on Graft Healing of Tomato[J]. Scientia Agricultura Sinica, 2022, 55(2): 365-377.

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参考文献 30

[1]	HUANG Y, KONG Q S, CHEN F, BIE Z L. The history, current status and future prospects of vegetable grafting in China. Acta Horticulturae, 2015, 1086:31-39.
[2]	FLORES F B, SANCHEZ-BEL P, ESTAN M T, MARTINEZ- RODRIGUEZ M M, MOYANO E, MORALES B, CAMPOS J F, GARCIA-ABELLÁN J O, EGEA M I, FERNÁNDEZ-GARCIA N, ROMOJARO F, BOLARÍN M C. The effectiveness of grafting to improve tomato fruit quality. Scientia Horticulturae, 2010, 125(3):211-217. doi: 10.1016/j.scienta.2010.03.026
[3]	王希波, 张祺恺, 张金亮, 张伟丽. 番茄双断根双头嫁接育苗关键技术. 中国蔬菜, 2019(6):98-100.
	WANG X B, ZHANG Q K, ZHANG J L, ZHANG W L. Key technology of tomato seedling cultivation by double-root-cutting and double-head grafting. China Vegetables, 2019(6):98-100. (in Chinese)
[4]	高丽红, 陈义, 田永强. 番茄一苗双头嫁接育苗关键技术. 农业工程技术, 2020, 40(4):22-24.
	GAO L H, CHEN Y, TIAN Y Q. Key technology of tomato seedling with double head grafting. Applied Engineering Technology, 2020, 40(4):22-24. (in Chinese)
[5]	NOTAGUCHI M, KUROTANI K I, SATO Y, TABATA R, KAWAKATSU Y, OKAYASU K, YU S W, OKADA R, ASAHINA M, ICHIHASHI Y, SHIRASU K, SUZUKI T, NIWA M, HIGASHIYAMA T. Cell-cell adhesion in plant grafting is facilitated by β-1, 4- glucanases. Science, 2020, 369(6504):698-702. doi: 10.1126/science.abc3710
[6]	MIAO L, LI Q, SUN T S, CHAI S, WANG C L, BAI L Q, SUN M T, LI Y S, QIN X, ZHANG Z H, YU X C. Sugars promote graft union development in the heterograft of cucumber onto pumpkin. Horticulture Research, 2021, 8(1):146. doi: 10.1038/s41438-021-00580-5
[7]	YEOMAN M M, KILPATRICK D C, MIEDZYBRODZKA M B, GOULD A R. Cellular interactions during graft formation in plants, a recognition phenomenon? Symposia of the Society for Experimental Biology, 1978, 32:139-160.
[8]	ALONI B, COHEN R, KARNI L, AKTAS H, EDELSTEIN M. Hormonal signaling in rootstock-scion interactions. Scientia Horticulturae, 2010, 127(2):119-126. doi: 10.1016/j.scienta.2010.09.003
[9]	LOUGH T J, LUCAS W J. Integrative plant biology: role of phloem long-distance macromolecular trafficking. Annual Review of Plant Biology, 2006, 57:203-232. doi: 10.1146/arplant.2006.57.issue-1
[10]	谢露露, 尚庆茂. 嫁接体植株中核酸与蛋白质的砧穗交流. 西北农业学报, 2019, 28(1):1-7.
	XIE L L, SHANG Q M. Interflows of nucleic acids and proteins between scion and stock in grafted plants. Acta Agriculturae Boreali-Occidentalis Sinica, 2019, 28(1):1-7. (in Chinese)
[11]	ASAHINA M, AZUMA K, PITAKSARINGKARN W, YAMAZAKI T, MITSUDA N, OHME-TAKAGI M, YAMAGUCHI S, KAMIYA Y, OKADA K, NISHIMURA T, KOSHIBA T, YOKOTA T, KAMADA H, SATOH S. Spatially selective hormonal control of RAP2.6L and ANAC071 transcription factors involved in tissue Reunion in Arabidopsis. PNAS, 2011, 108(38):16128-16132. doi: 10.1073/pnas.1110443108
[12]	PITAKSARINGKARN W, MATSUOKA K, ASAHINA M, MIURA K, SAGE-ONO K, ONO M, YOKOYAMA R, NISHITANI K, ISHII T, IWAI H, SATOH S. XTH20 and XTH19 regulated by ANAC071 under auxin flow are involved in cell proliferation in incised Arabidopsis inflorescence stems. The Plant Journal, 2014, 80(4):604-614. doi: 10.1111/tpj.12654
[13]	MELNYK C W, SCHUSTER C, LEYSER O, MEYEROWITZ E M. A developmental framework for graft formation and vascular reconnection in Arabidopsis thaliana. Current Biology, 2015, 25(10):1306-1318. doi: 10.1016/j.cub.2015.03.032
[14]	MATSUMOTO-KITANO M, KUSUMOTO T, TARKOWSKI P, KINOSHITA-TSUJIMURA K, VACLAVÍKOVA K, MIYAWAKI K, KAKIMOTO T. Cytokinins are central regulators of cambial activity. Proceedings of the National Academy of Sciences of the United States of America, 2009, 105(50):20027-20031.
[15]	BISHOPP A, HELP H, EL-SHOWK S, WEIJERS D, SCHERES B, FRIML J, BENKOVÁ E, MÄHÖNEN A P, HELARIUTTA Y. A mutually inhibitory interaction between auxin and cytokinin specifies vascular pattern in roots. Current Biology, 2011, 21(11):917-926. doi: 10.1016/j.cub.2011.04.017
[16]	MURARO D, MELLOR N, POUND M P, HELP H, LUCAS M, CHOPARD J, BYRNE H M, GODIN C, HODGMAN T C, KING J R, PRIDMORE T P, HELARIUTTA Y, BENNETT M J, BISHOPP A. Integration of hormonal signaling networks and mobile microRNAs is required for vascular patterning in Arabidopsis roots. PNAS, 2014, 111(2):857-862. doi: 10.1073/pnas.1221766111
[17]	DE RYBEL B, MÄHÖNEN A P, HELARIUTTA Y, WEIJERS D. Plant vascular development: From early specification to differentiation. Nature Reviews Molecular Cell Biology, 2015, 17:30. doi: 10.1038/nrm.2015.6
[18]	LU S F, SONG Y R. Relation between phytohormone level and vascular bridge differentiation in graft union of explanted internode autografting. Chinese Science Bulletin, 1999, 44(20):1874-1878. doi: 10.1007/BF02886344
[19]	CUI Q Q, XIE L L, DONG C J, GAO L H, SHANG Q M. Stage-specific events in tomato graft formation and the regulatory effects of auxin and cytokinin. Plant Science, 2021, 304:110803. doi: 10.1016/j.plantsci.2020.110803
[20]	XIE L L, DONG C J, SHANG Q M. Gene co-expression network analysis reveals pathways associated with graft healing by asymmetric profiling in tomato. BMC Plant Biology, 2019, 19(1):373. doi: 10.1186/s12870-019-1976-7
[21]	KÖSE C, GÜLERYÜZ M. Effects of auxins and cytokinins on graft union of grapevine (Vitis vinifera). New Zealand Journal of Crop and Horticultural Science, 2006, 34(2):145-150. doi: 10.1080/01140671.2006.9514399
[22]	MOGHADAM A R L, ARDEBILI Z O, REZAIE L. Effect of indole butyric acid on micrografting of cactus. African Journal of Biotechnology, 2012, 11(24):6484-6493.
[23]	WANG J, JIN Z, YIN H, YAN B, REN Z Z, XU J, MU C J, ZHANG Y, WANG M Q, LIU H. Auxin redistribution and shifts in PIN gene expression during Arabidopsis grafting. Russian Journal of Plant Physiology, 2014, 61(5):688-696. doi: 10.1134/S102144371405015X
[24]	SARAVANA KUMAR R M, GAO L X, YUAN H W, XU D B, LIANG Z, TAO S C, GUO W B, YAN D L, ZHENG B S, EDQVIST J. Auxin enhances grafting success in Carya cathayensis (Chinese hickory). Planta, 2018, 247(3):761-772. doi: 10.1007/s00425-017-2824-3
[25]	赵渊渊, 董春娟, 尚庆茂. 夜温对番茄套管嫁接苗愈合的影响. 西北植物学报, 2015, 35(3):493-499.
	ZHAO Y Y, DONG C J, SHANG Q M. Healing responses of tube grafted tomato plug seedlings under different night temperatures. Acta Botanica Boreali-Occidentalia Sinica, 2015, 35(3):493-499. (in Chinese)
[26]	蒋欣梅, 王波, 于锡宏, 吴凤芝, 张修国, 杨光鹏, 王欣. 双断根套管嫁接方法对番茄苗愈合及根系再生的影响. 东北农业大学学报, 2017, 48(9):21-27.
	JIANG X M, WANG B, YU X H, WU F Z, ZHANG X G, YANG G P, WANG X. Effect of both-root-cut tube grafting method on coalescence responses and root regenerated of tomato seeding. Journal of Northeast Agricultural University, 2017, 48(9):21-27. (in Chinese)
[27]	张雨欣, 王波, 刘在民, 于锡宏, 蒋欣梅. 双断根嫁接对番茄生长及果实产量、品质的影响. 长江蔬菜, 2018(14):57-59.
	ZHANG Y X, WANG B, LIU Z M, YU X H, JIANG X M. Effects of double root-cutting grafting on growth, fruit yield and quality of tomato. Journal of Changjiang Vegetables, 2018(14):57-59. (in Chinese)
[28]	ZHAO Y D. Auxin biosynthesis. The Arabidopsis Book, 2014, 12:e0173.
[29]	MELNYK C W, GABEL A, HARDCASTLE T J, ROBINSON S, MIYASHIMA S, GROSSE I, MEYEROWITZ E M. Transcriptome dynamics at Arabidopsis graft junctions reveal an intertissue recognition mechanism that activates vascular regeneration. PNAS, 2018, 115(10):E2447-E2456. doi: 10.1073/pnas.1718263115
[30]	BILYEU K D, COLE J L, LASKEY J G, RIEKHOF W R, ESPARZA T J, KRAMER M D, MORRIS R O. Molecular and biochemical characterization of a cytokinin oxidase from maize. Plant Physiology, 2001, 125(1):378-386. doi: 10.1104/pp.125.1.378

基因 Gene	登录号 Accession No.	引物序列（5′-3′） Primer sequence
SlIAA1	Solyc09g083280	SlIAA1-F: TGGATGGTGCCCCTTATCTA SlIAA1-R: ACAAGAAGACATAAACATTTCCCAA
SlPIN1	Solyc03g118740	SlPIN1-F: GGCAATTGTACAGGCAGCTC SlPIN1-R: CCAATGTAATCGGCAACGCA
SlHP1	Solyc01g080540	SlHP1-F: CAAGTGGAAATGGTGGGGAA SlHP1-R: ACCATCCAAGAATCCCTCACG
VND7	Solyc06g065410	VND7-F: ACAACAATGAAGATGATCGTGGCG VND7-R: CTGGGAAGCACTCAAGCAAATGG
NEN4	Solyc04g054720	NEN4-F: AACTGTCAAGGCACAACCAGC NEN4-R: CCCAAGCTCTGTCTTGCATAAG
PXY	Solyc03g093330	PXY-F: CTTATGGCTACATCGCACCTGAATA PXY-R: TTCCATCGCCAAATCCTGGT
Histone H4	Solyc11g066160	Histone H4-F: GGGAGGCAAAGGATTAGGCA Histone H4-R: GAACCCCACGAGTTTCCTCA
SlcycB1-4	Solyc01g009040	SlcycB1-4-F: AGAGGCGGGGAATAGTCGTC SlcycB1-4-R: ACAGCAGGAGCAATCACAAGG
Actin41	Solyc04g011500	SlActin-F: CTTCCAGCAGATGTGGATTGC SlActin-R: GCATCTCTGGTCCAGTAGGAAA

处理 Treatment	成活率 Survival rate (%)	萎蔫率 Wilting rate (%)
处理 Treatment	成活率 Survival rate (%)	6 d	7 d	8 d
CK	100±0.00a	2.78±0.03b	0.00±0.00b	0.00±0.00a
P	100±0.00a	8.33±0.00ab	2.78±0.03ab	0.00±0.00a
C	97.22±0.03a	11.11±0.06ab	5.56±0.03ab	2.78±0.03a
PC	94.44±0.03a	16.67±0.05a	11.11±0.03a	5.56±0.03a

断根与打顶对番茄嫁接愈合的抑制作用

Inhibiting Eeffect of Root-Cutting and Top-Pinching on Graft Healing of Tomato

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[15]	孙磊,王晓玥,王慧玲,闫爱玲,张国军,任建成,徐海英. 不同砧木对鲜食葡萄生长和香气品质的影响[J]. 中国农业科学, 2021, 54(20): 4405-4420.