中国农业科学 ›› 2019, Vol. 52 ›› Issue (15): 2604-2615.doi: 10.3864/j.issn.0578-1752.2019.15.005
收稿日期:
2018-12-25
接受日期:
2019-02-23
出版日期:
2019-08-01
发布日期:
2019-08-06
通讯作者:
袁玲
作者简介:
白如霞,E-mail:基金资助:
BAI RuXia,ZENG HuiWen,FAN Qian,YIN Jie,SUI ZongMing,YUAN Ling()
Received:
2018-12-25
Accepted:
2019-02-23
Online:
2019-08-01
Published:
2019-08-06
Contact:
Ling YUAN
摘要:
【目的】明确撕裂蜡孔菌(Ceriporia lacerata)对黄瓜的防病、促生作用,为农药、肥料减施增效提供依据。【方法】以自主分离的撕裂蜡孔菌HG2011新菌株为供试菌,采用Bonnet液体培养基制备撕裂蜡孔菌发酵液(C. lacerata fermentation broth,CLB),另利用蛭石、玉米粉和谷壳等制备撕裂蜡孔菌固体菌剂(C. lacerata solid agent,CLA),通过拮抗、对峙培养、盆栽试验和田间试验,研究撕裂蜡孔菌对甜瓜球腔菌(Mycosphaerella melonis)引起的黄瓜蔓枯病的防治作用,以及对黄瓜生长发育、养分吸收、土壤酶活性、黄瓜产量和果实品质的影响。【结果】在拮抗试验中,培养第6天50% CLB对甜瓜球腔菌的抑制率为32.39%,与甲基托布津(thiophanate methyl,TM)作用相当。在对峙培养试验中,甜瓜球腔菌生长受到撕裂蜡孔菌抑制,撕裂蜡孔菌则继续生长至完全覆盖甜瓜球腔菌,使之变形、萎缩和消失。在盆栽试验中,喷病菌孢子液(pathogen inoculation,PI)处理的发病率为36.67%,病情指数为38.40。与PI相比,CLB可显著降低蔓枯病的发病率和病情指数,其相对防治效果为79.69%,同样与甲基托布津(75.57%)相当。与常规施化肥(CF)相比,施用CLB可促进黄瓜植株生长,提高生物量、根系活力和叶绿素含量,分别提高了5.87%—21.45%、36.50%—38.83%和10.54%—19.80%;黄瓜植株养分吸收量分别增加45.24%—69.05%(氮)、20.51%—43.59%(磷)和19.88%—38.51%(钾);土壤脲酶、酸性磷酸酶、过氧化氢酶、纤维素酶、脱氢酶和蛋白酶活性增强,增加幅度分别为8.73%—35.84%、7.55%—10.74%、25.32%—26.49%、186.21%—279.23%、47.99%—76.51%和49.00%—100.00%,施用高量(150 mL)CLB处理的效果优于低量(75 mL)CLB处理的效果。在田间试验中,与常规施肥相比,常规施肥与固体菌剂配施(CF+CLA10)与减肥处理与固体菌剂配施(75% CF+CLA10)均显著提高了黄瓜单株结果数、产量和游离氨基酸含量,增幅分别为13.61%、13.87%、71.54%(CF+CLA10)和11.51%、11.71%、54.37%(75% CF+CLA10),此外,75% CF+CLA10处理显著降低了硝酸盐含量,降幅为14.93%。【结论】撕裂蜡孔菌HG2011可抑制甜瓜球腔菌生长。喷施CLB能防治黄瓜蔓枯病,降低发病率和病情指数,提高防治效果;盆栽施加CLB可提高土壤酶活性,促进黄瓜幼苗吸收养分,使黄瓜健康生长。田间施用CLA可增加黄瓜产量,提高黄瓜果实游离氨基酸含量,降低硝酸盐含量,改善品质,益于实现减肥增效。撕裂蜡孔菌HG2011能分解木质素和纤维素,在作物秸秆中生长迅速,利用该生物菌剂制作堆肥可兼具防病、促生效果。
白如霞,曾汇文,范倩,殷洁,隋宗明,袁玲. 撕裂蜡孔菌对黄瓜蔓枯病的防治作用及促生增产效果[J]. 中国农业科学, 2019, 52(15): 2604-2615.
BAI RuXia,ZENG HuiWen,FAN Qian,YIN Jie,SUI ZongMing,YUAN Ling. Effects of Ceriporia lacerata on Gummy Stem Blight Control, Growth Promotion and Yield Increase of Cucumbers[J]. Scientia Agricultura Sinica, 2019, 52(15): 2604-2615.
表1
CLB对黄瓜蔓枯病的防治效果及相关生理指标的影响"
处理 Treatment | 病情指数 Disease index | 发病率 Incidence (%) | 防治效果 Control efficacy (%) | 丙二醛含量 Malondialdehyde content (μmol·g-1) | 相对电导率 Relative electric conductivity (%) |
---|---|---|---|---|---|
CK | 0c | 0c | — | 3.95±0.03d | 100.00±0.58c |
PI | 38.40±1.11a | 36.67±3.33a | 0b | 8.14±1.37a | 148.55±3.79a |
TM+PI | 9.38±1.20b | 10.00±0b | 75.57±3.11a | 5.47±0.26b | 129.48±5.57b |
CLB+PI | 7.80±0.92b | 10.00±3.33b | 79.69±2.40a | 5.03±0.33c | 128.32±6.09b |
表2
不同处理下黄瓜植株养分含量与吸收量"
处理 Treatment | 含量 Content (%) | 吸收量 Absorption (g/plant) | |||||
---|---|---|---|---|---|---|---|
氮N | 磷P | 钾K | 氮N | 磷P | 钾K | ||
CK | 1.36±0.06b | 1.34±0.01c | 4.86±0.09c | 0.30±0.05d | 0.30±0.04d | 1.10±0.12d | |
CF | 1.46±0.03b | 1.35±0.03c | 5.57±0.21c | 0.42±0.03c | 0.39±0.03c | 1.61±0.10c | |
CF+CLB75 | 1.88±0.10a | 1.46±0.04b | 5.95±0.12b | 0.61±0.03b | 0.47±0.06b | 1.93±0.25b | |
CF+CLB150 | 1.94±0.07a | 1.52±0.03a | 6.27±0.05a | 0.71±0.03a | 0.56±0.01a | 2.23±0.17a |
表3
CLA 对黄瓜产量及品质的影响"
处理 Treatment | 单果重 Fruit weight (g) | 单株结果数 Fruit quantity of plant | 产量 Yield (kg·667 m-2) | 可溶性糖 Soluble sugar (%) | 维生素C Vitamin C (mg·100 g-1) | 可溶性蛋白 Soluble protein (mg·100 g-1) | 游离氨基酸 Free amino acid (mg·100 g-1) | 硝酸盐 Nitrate (μg·g-1) |
---|---|---|---|---|---|---|---|---|
CK | 210.42±14.76b | 3.68±0.17c | 2709.58±172.18c | 2.30±0.08a | 7.40±0.46b | 98.32±5.88a | 54.16±5.12b | 59.76±2.24c |
CF | 241.77±11.21a | 6.17±0.13b | 5216.28±210.63b | 2.66±0.30a | 8.29±0.34a | 101.57±8.23a | 52.36±2.21b | 80.20±6.64a |
CF+CLA10 | 241.58±6.98a | 7.01±0.29a | 5939.75±412.63a | 2.69±0.07a | 8.59±0.64a | 100.60±9.58a | 89.82±4.24a | 75.11±2.01ab |
75% CF+CLA10 | 233.86±16.79a | 6.88±0.31a | 5826.92±411.05a | 2.66±0.34a | 8.71±0.32a | 103.77±11.63a | 80.83±11.58a | 68.23±6.79bc |
[1] | ST AMAND P C, WEHNER T C . Generation means analysis of leaf and stem resistance to gummy stem blight in cucumber. Journal of the American Society for Horticultural Science, 2001,126(1):95-99. |
[2] | WEHNER T C, ST AMAND P C . Field tests for cucumber resistance to gummy stem blight in North Carolina. HortScience, 1993,28(4):327-329. |
[3] | 李英 . 瓜类蔓枯病菌的生物学特性和黄瓜抗病资源的筛选[D]. 南京: 南京农业大学, 2007. |
LI Y . Study on biology characteristics of Didymella bryoniae and screening of resistance germplasm of cucumber[D]. Nanjing: Nanjing Agricultural University, 2007. (in Chinese) | |
[4] | 孙元超 . 怎样防治黄瓜蔓枯病. 现代农村科技, 2010(17):24-25. |
SUN Y C . How to control gummy stem blight in cucumber. Modern Agricultural Science and Technology, 2010(17):24-25. (in Chinese) | |
[5] | 吴建寨, 韩书庆 . 黄瓜2016年市场分析及2017年市场预测. . |
WU J Z, HAN S Q. Market analysis of cucumber in 2016 and market forecast in 2017. (in Chinese) | |
[6] | SHARMA V, SALWAN R, SHARMA P N, KANWAR S S . Elucidation of biocontrol mechanisms of Trichoderma harzianum against different plant fungal pathogens: Universal yet host specific response. International Journal of Biological Macromolecules, 2017,95:72-79. |
[7] | 殷洁, 袁玲 . 寡雄腐霉菌剂对辣椒疫病的防治及促生效应. 园艺学报, 2017,44(12):2327-2337. |
YIN J, YUAN L . Phytophthora disease control and growth promotion of pepper by Pythium oligandrum. Acta Horticulturae Sinica, 2017,44(12):2327-2337. (in Chinese) | |
[8] | GHOLAMI A, SHAHSAVANI S, NEZARAT S . The effect of plant growth promoting Rhizobacteria(PGPR) on germination, seedling growth and yield of maize. Proceedings of World Academy of Science: Engineering and Technology, 2009,49(1):19-24. |
[9] | ARSENEAULT T, GOYER C, FILION M . Pseudomonas fluorescens LBUM223 increases potato yield and reduces common scab symptoms in the field. Phytopathology, 2015,105(10):1311-1317. |
[10] | 彭于发 . 荧光假单胞菌Tn5诱变防病增产研究初报. 中国农业科学, 1990,23(1):88-89. |
PENG Y F . Effects of Pseudomonas fluorescens mutated by Tn5 on disease suppression and crop yield increase. Scientia Agricultura Sinica, 1990,23(1):88-89. (in Chinese) | |
[11] | SOWMYA D S, RAO M S, KUMAR R M, GAVASKAR J, PRITI K . Bio-management of Meloidogyne incognita and Erwinia carotovora in carrot(Daucus carota L.) using Pseudomonas putida and Paecilomyces lilacinus. Nematologia Mediterranea, 2012,40:189-194. |
[12] |
段佳丽, 薛泉宏, 舒志明, 王东胜, 何斐 . 放线菌Act12与腐植酸钾配施对丹参生长及其根域微生态的影响. 生态学报, 2015,35(6):1807-1819.
doi: 10.5846/stxb201305231154 |
DUAN J L, XUE Q H, SHU Z M, WANG D S, HE F . Effects of combined application of actinomycetes Act12 bio-control agents and potassium humate on growth and microbial flora in rooting zone of Salvia miltiorrhiza Bge. Acta Ecologica Sinica, 2015,35(6):1807-1819. (in Chinese)
doi: 10.5846/stxb201305231154 |
|
[13] | JANG Y, CHOI H E, LIM Y W, LEE J S, KIM J J . The first report ofCeriporia lacerate(Phanerochaetaceae, Basidiomycota) in Korea. Mycotaxon, 2012,119(1):397-403. |
[14] | YUAN H S . A new species of Junghuhnia(Basidiomycota, Meruliaceae) from tropical China. Mycotaxon, 2011,117(1):255-260. |
[15] | 贾碧丝 . 中国蜡孔菌属分类与系统发育研究[D]. 北京: 北京林业大学, 2012. |
JIA B S . Taxonomy and phylogeny of Ceriporia in China[D]. Beijing: Beijing Forestry University, 2012. (in Chinese) | |
[16] | JIA B S, ZHOU L W, CUI B K, RIVOIRE B, DAI Y C . Taxonomy and phylogeny of Ceriporia(Polyporales, Basidiomycota) with an emphasis of Chinese collections. Mycological Progress, 2014,13(1):81-93. |
[17] | SHIN E J, KIM J E, KIM J H, PARK Y M, YOON S K, JANG B C, LEE S P, KIM B C . Hypoglycemic effects of submerged culture of Ceriporia lacerata mycelium. Korean Journal of Food Preservation, 2015,22(1):145-153. |
[18] | 王娜, 于圣, 褚衍亮, 徐翔宇, 林陈强 . 撕裂蜡孔菌在开放体系中对甲基橙染料的静态脱色研究. 菌物学报, 2015,34(6):1196-1204. |
WANG N, YU S, CHU Y L, XU X Y, LIN C Q . Decolorization of methyl orange dye by Ceriporia lacerata under statically air-opened condition. Mycosystema, 2015,34(6):1196-1204. (in Chinese) | |
[19] | WANG N, CHU Y L, WU F, ZHAO Z L, XU X Y . Decolorization and degradation of Congo red by a newly isolated white rot fungus,Ceriporia lacerata, from decayed mulberry branches. International Biodeterioration and Biodegradation, 2017,117:236-244. |
[20] | 黄建国, 殷洁, 袁玲 . 一株撕裂蜡孔菌及其防治作物真菌病害的应用: CN 107201317 A[P]. ( 2017-09-26) [2018-12-25]. |
HUANG J G, YIN J, YUAN L . Disease control by application of a strain of Ceriporia lacerate: CN 107201317 A[P]. ( 2017-09-26) [2018-12-25]. (in Chinese) | |
[21] | 袁玲, 殷洁, 黄建国 . 一株撕裂蜡孔菌的促生作用及应用: CN 107164245 A[P]. ( 2017 -09-15) [2018-12-25]. |
YUAN L, YIN J HUANG J G, . Growth promotion by application of a strain of Ceriporia lacerate: CN 107164245 A[P]. (2017 -09-15) [2018-12-25]. (in Chinese) | |
[22] | 殷洁, 范倩, 黄建国 . 撕裂蜡孔菌的新功能——防治茄子绵疫病及促生效应. 中国农业科学, 2018,51(12):2300-2310. |
YIN J, FAN Q, HUANG J G . New functions of Ceriporia lacerate in phytophthora blight control and growth promotion of eggplants. Scientia Agricultura Sinica, 2018,51(12):2300-2310. (in Chinese) | |
[23] | DIK A J, KONING G, KÖHL J . Evaluation of microbial antagonists for biological control of Botrytis cinerea stem infection in cucumber and tomato. European Journal of Plant Pathology, 1999,105(2):115-122. |
[24] | 何金环, 连艳鲜 . 生物化学实验技术. 2版. 北京: 中国轻工业出版社, 2014: 156-158. |
HE J H, LIAN Y X. Biochemistry Experiment and Technology. 2nd ed. Beijing: China Light Industry Press, 2014: 156-158. (in Chinese) | |
[25] | 杨剑虹, 王成林, 代亨林 . 土壤农化分析与环境监测. 北京: 中国大地出版社, 2008: 282-287. |
YANG J H, WANG C L, DAI H L. Soil Chemical Analysis and Environmental Monitoring . Beijing: China Earth Press, 2008: 282-287. (in Chinese) | |
[26] | 关松荫 . 土壤酶及其研究法. 北京: 农业出版社, 1986: 274-339. |
GUAN S Y. Soil Enzymes and Its Research Methods. Beijing: Agriculture Press, 1986: 274-339. (in Chinese) | |
[27] | AGROPAGES . Global pesticide market - by regions and vendors - market size, demand forecasts, industry trends and updates, supplier market shares 2014-2020. . |
[28] | 贾振华, 李静, 贾中雄 . 化学农药在我国农作物生产中的应用与分析//陈万全. 病虫害绿色防控与农产品质量安全——中国植物保护学会2015年学术年会论文集. 北京: 中国农业科学技术出版社, 2015: 319-322. |
JIA Z H, LI J, JIA Z X. Application and analysis of chemical pesticides in crop production in China//CHEN W Q. Green Prevention and Control of Diseases and Insect Pests and Quality Safety of Agricultural Products——Proceedings of the 2015 Annual Convention of China Society of Plant Protection. Beijing: China Agricultural Science and Technology Press, 2015: 319-322. (in Chinese) | |
[29] | 雷仲仁 . 病虫害生物防治是实现蔬菜安全生产的主要途径. 中国农业科学, 2016,49(15):2932-2934. |
LEI Z R . Biological control of diseases and insect pests is valid method to ensure vegetable safe producing. Scientia Agricultura Sinica, 2016,49(15):2932-2934. (in Chinese) | |
[30] | O’BRIEN P A . Biological control of plant diseases. Australasian Plant Pathology, 2017,46:293-304. |
[31] | BROGLIE K, CHET I, HOLLIDAY M, CRESSMAN R, BIDDLE P, KNOWLTON S, MAUVAIS C J, BROGLIE R . Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science, 1991,254(5035):1194-1197. |
[32] | PICARD K, TIRILLY Y, BENHAMOU N . Cytological effects of cellulases in the parasitism of Phytophthora parasitica by Pythium oligandrum. Applied and Environmental Microbiology, 2000,66(10):4305-4314. |
[33] | MAUCH F, MAUCH-MANI B, BOLLER T . Antifungal hydrolases in pea tissue: Ⅱ. Inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase. Plant Physiology, 1988,88(3):936-942. |
[34] | GEREMIA R A, GOLDMAN G H, JACOBS D, ARDRTES W, VILA S B, VAN MONTAGU M, HERRERA-ESTRELLA A . Molecular characterization of the proteinase-encoding gene,prb1, related to mycoparasitism by Trichoderma harzianum. Molecular Microbiology, 1993,8(3):603-613. |
[35] | 管炜, 李淑菊, 王惠哲, 杨瑞环 . 几种杀菌剂对黄瓜蔓枯病菌的室内毒力测定. 天津农业科学, 2010,16(3):82-83. |
GUAN W, LI S J, WANG H Z, YANG R H . Toxicity of some fungicides to Mycosphaerella melonis. Tianjin Agricultural Sciences, 2010,16(3):82-83. (in Chinese) | |
[36] |
宋锐, 林丽果, 王康英, 宋浩然, 蒋勇斌, 刘慧霞 . 不同盐生境下硅对高羊茅生物量及生理生化特征的影响. 草业学报, 2016,25(8):91-97.
doi: 10.11686/cyxb2015501 |
SONG R, LIN L G, WANG K Y, SONG H R, JIANG Y B, LIU H X . Effects of silicon supply on the biomass and physiochemical features of tall fescue seedlings under different salinization conditions. Acta Prataculturae Sinica, 2016,25(8):91-97. (in Chinese)
doi: 10.11686/cyxb2015501 |
|
[37] |
李书田, 金继运 . 中国不同区域农田养分输入、输出与平衡. 中国农业科学, 2011,44(20):4207-4229.
doi: 10.3864/j.issn.0578-1752.2011.20.009 |
LI S T, JIN J Y . Characteristics of nutrient input/output and nutrient balance in different regions of China. Scientia Agricultura Sinica, 2011,44(20):4207-4229. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2011.20.009 |
|
[38] | 王克安 . 设施蔬菜高效施肥与土壤无害化处理. 北京: 金盾出版社, 2015. |
WANG K A. High Efficiency Fertilization for Vegetable Plants and Harmlessniss Treatment of Soil. Beijing: Jindun Publishing House, 2015. (in Chinese) | |
[39] | JOHNSON S E, LOEPPERT R H . Role of organic acids in phosphate mobilization from iron oxide. Soil Science Society of America Journal, 2006,70(1):222-234. |
[40] |
刘丽, 梁成华, 王琦, 杜立宇, 吴玉梅, 韩巍 . 低分子量有机酸对土壤磷活化影响的研究. 植物营养与肥料学报, 2009,15(3):593-600.
doi: 10.11674/zwyf.2009.0315 |
LIU L, LIANG C H, WANG Q, DU L Y, WU Y M, HAN W . Effects of low-molecular-weight organic acids on soil phosphorus release. Plant Nutrition and Fertilizer Science, 2009,15(3):593-600. (in Chinese)
doi: 10.11674/zwyf.2009.0315 |
|
[41] | 王东升, 王君 . 低分子量有机酸作用下土壤矿物钾释放机制. 辽宁工程技术大学学报(自然科学版), 2009,28(Suppl. 2):259-261. |
WANG D S, WANG J . Mechanism of soil mineral potassium release extracted by low-molecular-weigh organic acids. Journal of Liaoning Technical University (Natural Science), 2009,28(Suppl. 2):259-261. (in Chinese) | |
[42] |
杨扬, 高克祥, 吴岩, 刘晓光 . 吲哚乙酸跨界信号调节植物与细菌互作. 生物技术通报, 2016,32(8):14-21.
doi: 10.13560/j.cnki.biotech.bull.1985.2016.08.003 |
YANG Y, GAO K X, WU Y, LIU X G . Indole-3-acetic acid-mediated cross-kingdom signalling involved in plant-bacteria interactions. Biotechnology Bulletin, 2016,32(8):14-21. (in Chinese)
doi: 10.13560/j.cnki.biotech.bull.1985.2016.08.003 |
|
[43] | KLOEPPER J W, LEONG J, TEINTZE M, SCHROTH M N . Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria. Nature, 1980,286(2):885-886. |
[44] | 徐凯, 郭延平, 张上隆 . 不同光质对草莓叶片光合作用和叶绿素荧光的影响. 中国农业科学, 2005,38(2):369-375. |
XU K, GUO Y P, ZHANG S L . Effect of light quality on photosynthesis and chlorophyll fluorescence in strawberry leaves. Scientia Agricultura Sinica, 2005,38(2):369-375. (in Chinese) | |
[45] | 孙洪助 . 红蓝光比例对绿叶蔬菜生理特性及品质的影响[D]. 南京: 南京农业大学, 2014. |
SUN H Z . Effects of proportions of red and blue light on physiological characteristics and quality in leafy greens[D]. Nanjing: Nanjing Agricultural University, 2014. (in Chinese) | |
[46] | 王素平, 郭世荣, 李璟, 胡晓辉, 焦彦生 . 盐胁迫对黄瓜幼苗根系生长和水分利用的影响. 应用生态学报, 2006,17(10):1883-1888. |
WANG S P, GUO S R, LI J, HU X H, JIAO Y S . Effects of salt stress on the root growth and leaf water use efficiency of cucumber seedlings. Chinese Journal of Applied Ecology, 2006,17(10):1883-1888. (in Chinese) | |
[47] | 张丽娟, 曲继松, 朱倩楠, 吴涛 . 不同剂量外源纤维素酶对设施土壤生物活性与番茄生长的影响. 植物营养与肥料学报, 2017,23(4):1089-1094. |
ZHANG L J, QU J S, ZHU Q N, WU T . Effects of exogenous cellulase with different dosages on the biological activity and tomato growth in greenhouse soil. Journal of Plant Nutrition and Fertilizer, 2017,23(4):1089-1094. (in Chinese) | |
[48] |
杨丽娟, 须晖, 邱忠祥, 刘永青 . 菜田土壤酶活性与黄瓜产量的关系. 植物营养与肥料学报, 2000,6(1):113-116.
doi: 10.11674/zwyf.2000.0117 |
YANG L J, XU H, QIU Z X, LIU Y Q . Relationship between activities of enzyme and cucumber yield in vegetable soil. Plant Nutrition and Fertilizer Science, 2000,6(1):113-116. (in Chinese)
doi: 10.11674/zwyf.2000.0117 |
|
[49] | DE LA PAZ JIMENEZ M, DE LA HORRA A, PMZZO L, PALMA R M . Soil quality: a new index based on microbiological and biochemical parameters. Biology and Fertility of Soils, 2002,35:302-306. |
[50] | 申卫收, 林先贵, 张华勇, 尹睿, 段增强, 施卫明 . 不同施肥处理下蔬菜塑料大棚土壤微生物活性及功能多样性. 生态学报, 2008,28(6):2682-2689. |
SHEN W S, LIN X G, ZHANG H Y, YIN R, DUAN Z Q, SHI W M . Microbial activity and functional diversity in soils used for the commercial production of cucumbers and tomatoes in polytunnel greenhouse, under different fertilization. Acta Ecologica Sinica, 2008,28(6):2682-2689. (in Chinese) |
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