中国农业科学 ›› 2020, Vol. 53 ›› Issue (1): 160-171.doi: 10.3864/j.issn.0578-1752.2020.01.015

• 专题:黄瓜分子生物学 • 上一篇    下一篇

黄瓜单性结实性状遗传与QTL定位

牛志红,宋晓飞,李晓丽,郭晓雨,何书强,贺栾劲芝,冯志红,孙成振(),闫立英()   

  1. 河北科技师范学院,河北秦皇岛 066004
  • 收稿日期:2019-01-21 接受日期:2019-06-05 出版日期:2020-01-01 发布日期:2020-01-19
  • 通讯作者: 孙成振,闫立英
  • 作者简介:牛志红,Tel:18332567462;E-mail:zhihongn@163.com。|宋晓飞,Tel:13613358355;E-mail:songxiaofei1979@163.com。
  • 基金资助:
    河北省重点研发计划(19226364D);河北省现代农业产业技术体系创新团队建设项目(HBCT2018030209)

Inheritance and QTL Mapping for Parthenocarpy in Cucumber

ZhiHong NIU,XiaoFei SONG,XiaoLi LI,XiaoYu GUO,ShuQiang HE,LuanJingZhi HE,ZhiHong FENG,ChengZhen SUN(),LiYing YAN()   

  1. Hebei Normal University of Science and Technology, Qinhuangdao 066004, Hebei
  • Received:2019-01-21 Accepted:2019-06-05 Online:2020-01-01 Published:2020-01-19
  • Contact: ChengZhen SUN,LiYing YAN

摘要:

【目的】单性结实性是影响设施黄瓜产量和品质的重要性状。深入解析黄瓜单性结实性状遗传规律并对其进行QTL定位,有助于提高设施专用黄瓜品种育种效率。【方法】以强单性结实自交系‘6457’和弱单性结实自交系‘6426’构建的重组自交系F2:8为材料,基于3年表型数据,采用黄瓜基因组测序SSR分子标记构建黄瓜遗传连锁图谱,结合QTL-Seq分析,对黄瓜单性结实性进行QTL定位。【结果】黄瓜单性结实性状符合数量遗传特征。利用SSR标记构建了1张包含11个连锁群的遗传图谱,覆盖基因组555.0 cM,平均图距为6.8 cM。2016—2018年春季在3号染色体上均检测到1个与黄瓜单性结实性相关的QTL位点,位于标记SSR19430和SSR15419之间(3.33—5.57 Mb),遗传距离6.6 cM,贡献率分别为11%、12.5%和6.3%。进一步进行QTL-Seq分析,发现4个与黄瓜单性结实性相关的QTL,分别位于1号(4.38—11.00 Mb)、3号(2.24—10.66 Mb)、6号(15.67—17.93 Mb,26.33—27.49 Mb)染色体上。其中在3号染色体上检测到的QTL与Map QTL所得的QTL区间重叠。推测Csa3G047740Csa3G073810、Csa3G043910Csa6G362930为与黄瓜单性结实性状相关的候选基因。【结论】分别在1、3、6号染色体上检测到4个与黄瓜单性结实性相关的QTL位点,其中3号染色体上的QTL年度间稳定,贡献率较高。

关键词: 黄瓜, 单性结实, 遗传图谱, QTL定位

Abstract:

【Objective】 Parthenocarpy is an important trait affecting both yield and quality in the protected cucumber production. Genetic analysis of parthenocarpy and its QTL mapping are of great significance for improving breeding efficiency in cucumber.【Method】 Based on three-year phenotypic data, the genetic linkage map of cucumber was constructed by using SSR molecular markers obtained from cucumber genome sequencing, and the QTL-Seq analysis was used to map the parthenocarpy of cucumber, using recombinant inbred line F2:8 constructed from strong parthenocarpic inbred line ‘6457’ and weak parthenocarpic inbred line ‘6426’.【Result】 The inheritance of parthenocarpy in cucumber accorded with quantitative inheritance. A genetic map containing 11 linkage groups was constructed using SSR markers, covering 555.0 cM of the genome with an average distance of 6.8 cM. In the spring of 2016, 2017 and 2018, a single QTL locus related to parthenocarpy was commonly detected between SSR19430 and SSR15419 markers (3.33-5.57 Mb) on chromosome 3, and the genetic distance was 6.6 cM, with 11%, 12.5% and 6.3% contribution rate, respectively. By QTL-Seq analysis, four QTLs related to parthenocarpy were identified on chromosomes 1 (4.38-11.00 Mb), 3 (2.24-10.66 Mb) and 6 (15.67-17.93 Mb; 26.33-27.49 Mb), the QTL on chromosome 3 overlapped with that detected by Map QTL approach. Csa3G047740, Csa3G073810, Csa3G043910 and Csa6G362930 were proposed to be candidate genes associated with parthenocarpic trait in cucumber. 【Conclusion】Four QTLs were identified on chromosome 1, 3 and 6, including one QTL on chromosome 3 that was consistently detected over years with relatively high contribution. The results could facilitate marker-assisted selection and understanding the underlying mechanism of parthenocarpy in cucumber.

Key words: cucumber, parthenocarpy, genetic map, QTL mapping