撕裂蜡孔菌对黄瓜蔓枯病的防治作用及促生增产效果

doi:10.3864/j.issn.0578-1752.2019.15.005

摘要/Abstract

摘要：

【目的】明确撕裂蜡孔菌（Ceriporia lacerata）对黄瓜的防病、促生作用,为农药、肥料减施增效提供依据。【方法】以自主分离的撕裂蜡孔菌HG2011新菌株为供试菌,采用Bonnet液体培养基制备撕裂蜡孔菌发酵液（C. lacerata fermentation broth,CLB）,另利用蛭石、玉米粉和谷壳等制备撕裂蜡孔菌固体菌剂（C. lacerata solid agent,CLA）,通过拮抗、对峙培养、盆栽试验和田间试验,研究撕裂蜡孔菌对甜瓜球腔菌（Mycosphaerella melonis）引起的黄瓜蔓枯病的防治作用,以及对黄瓜生长发育、养分吸收、土壤酶活性、黄瓜产量和果实品质的影响。【结果】在拮抗试验中,培养第6天50% CLB对甜瓜球腔菌的抑制率为32.39%,与甲基托布津（thiophanate methyl,TM）作用相当。在对峙培养试验中,甜瓜球腔菌生长受到撕裂蜡孔菌抑制,撕裂蜡孔菌则继续生长至完全覆盖甜瓜球腔菌,使之变形、萎缩和消失。在盆栽试验中,喷病菌孢子液（pathogen inoculation,PI）处理的发病率为36.67%,病情指数为38.40。与PI相比,CLB可显著降低蔓枯病的发病率和病情指数,其相对防治效果为79.69%,同样与甲基托布津（75.57%）相当。与常规施化肥（CF）相比,施用CLB可促进黄瓜植株生长,提高生物量、根系活力和叶绿素含量,分别提高了5.87%—21.45%、36.50%—38.83%和10.54%—19.80%;黄瓜植株养分吸收量分别增加45.24%—69.05%（氮）、20.51%—43.59%（磷）和19.88%—38.51%（钾）;土壤脲酶、酸性磷酸酶、过氧化氢酶、纤维素酶、脱氢酶和蛋白酶活性增强,增加幅度分别为8.73%—35.84%、7.55%—10.74%、25.32%—26.49%、186.21%—279.23%、47.99%—76.51%和49.00%—100.00%,施用高量（150 mL）CLB处理的效果优于低量（75 mL）CLB处理的效果。在田间试验中,与常规施肥相比,常规施肥与固体菌剂配施（CF+CLA₁₀）与减肥处理与固体菌剂配施（75% CF+CLA₁₀）均显著提高了黄瓜单株结果数、产量和游离氨基酸含量,增幅分别为13.61%、13.87%、71.54%（CF+CLA₁₀）和11.51%、11.71%、54.37%（75% CF+CLA₁₀）,此外,75% CF+CLA₁₀处理显著降低了硝酸盐含量,降幅为14.93%。【结论】撕裂蜡孔菌HG2011可抑制甜瓜球腔菌生长。喷施CLB能防治黄瓜蔓枯病,降低发病率和病情指数,提高防治效果;盆栽施加CLB可提高土壤酶活性,促进黄瓜幼苗吸收养分,使黄瓜健康生长。田间施用CLA可增加黄瓜产量,提高黄瓜果实游离氨基酸含量,降低硝酸盐含量,改善品质,益于实现减肥增效。撕裂蜡孔菌HG2011能分解木质素和纤维素,在作物秸秆中生长迅速,利用该生物菌剂制作堆肥可兼具防病、促生效果。

关键词: 撕裂蜡孔菌, 甜瓜球腔菌, 黄瓜, 蔓枯病, 防治作用, 促生

Abstract:

【Objective】The objective of this study is to clarify the effects of Ceriporia lacerata on plant disease control and growth promotion, and to provide a basis for reducing the application of chemical pesticides and fertilizers.【Method】A new self-isolated C. lacerata (fungal strain HG2011) was grown in Bonnet liquid medium and mixture made of vermiculite, maize powder, and rice husk, respectively, to produce culture broth (CLB) and solid inoculant (CLA). CLB and CLA were prepared and conduced to evaluate the effect of C. lacerata on the antagonistic activity against Mycosphaerella melonis, control of gummy stem blight, vegetative growth of cucumber seedlings, yield of cucumbers, soil enzyme activity, and quality of cucumbers with the method of antagonistic assay, confront culture, greenhouse pot experiments, and field experiments, respectively.【Result】In the antagonistic assay, the inhibition rate of 50% CLB against M. melonis was 32.39% in agar medium at the 6th day, which was similar to that of thiophanate methyl (TM). In the confront culture assay, C. lacerata HG2011 inhibited the growth of M. melonis, this antagonistic fungus could cover M. melonis colonies and make the hyphae deformed, shrunken and disappeared. In greenhouse pot experiments, the incidence of pathogen inoculation (PI) treatment was 36.67% and the disease index was 38.40. Compared with PI, CLB could significantly reduce the incidence and disease index of gummy stem blight, and the relative control efficacy was 79.69%, which was also similar to that of TM (75.57%). Compared with single conventional fertilization (CF), the application of CLB could promote the seedling growth, increase the biomass, root activity and chlorophyll content in leaves by 5.87%-21.45%, 36.50%-38.83% and 10.54%-19.80%, respectively. The nutrient uptake by cucumber seedlings increased by 45.24%-69.05% (nitrogen), 20.51%-43.59% (phosphorus), and 19.88%-38.51% (potassium), respectively. The activities of urease, acid phosphatase, catalase, cellulase, dehydrogenase, and protease increased by 8.73%-35.84%, 7.55%-10.74%, 25.32%-26.49%, 186.21%-279.23%, 47.99%-76.51% and 49.00%-100.00%. The effect of high dose (150 mL) CLB treatment was better than that of low dose (75 mL) CLB treatment. In field experiments, application of CLA on the basis of CF (CF+CLA₁₀) increased fruit quantity of plant by 13.61%, yield by 13.87%, and free amino acids content by 71.54%. Application of CLA on the basis of reducing 25% CF (75% CF+CLA₁₀) increased fruit quantity of plant by 11.51%, yield by 11.71%, and free amino acids content by 54.37%. In addition, compared with CF, 75% CF+CLA₁₀ significantly decreased nitrate content by 14.93%. 【Conclusion】 C. lacerata HG2011 strain can inhibit hyphal growth of M. melonis. Spraying CLB can control gummy stem blight, reduce the incidence and disease index, and improve the control efficacy. Pot application of CLB can increase the activity of soil enzyme, promote the absorption of nutrients by cucumber seedlings, and make the healthy growth of cucumber. Field application of CLA can increase the yield of cucumber and the content of free amino acids in fruits, reduce the content of nitrate content and improve the quality, which is beneficial to reduce application and increase efficiency of chemical fertilizer. C. lacerata HG2011 strain can decompose lignin and cellulose, and grow rapidly in crop straw. Composting with this biological agent can both prevent disease and promote growth.

Key words: Ceriporia lacerata, Mycosphaerella melonis, cucumber, gummy stem blight, control efficacy, growth promotion

白如霞,曾汇文,范倩,殷洁,隋宗明,袁玲. 撕裂蜡孔菌对黄瓜蔓枯病的防治作用及促生增产效果[J]. 中国农业科学, 2019, 52(15): 2604-2615.

BAI RuXia,ZENG HuiWen,FAN Qian,YIN Jie,SUI ZongMing,YUAN Ling. Effects of Ceriporia lacerata on Gummy Stem Blight Control, Growth Promotion and Yield Increase of Cucumbers[J]. Scientia Agricultura Sinica, 2019, 52(15): 2604-2615.

图/表 8

图1

图2

图3

表1

图4

表2

图5

表3

参考文献 50

[1]	ST AMAND P C, WEHNER T C . Generation means analysis of leaf and stem resistance to gummy stem blight in cucumber. Journal of the American Society for Horticultural Science, 2001,126(1):95-99.
[2]	WEHNER T C, ST AMAND P C . Field tests for cucumber resistance to gummy stem blight in North Carolina. HortScience, 1993,28(4):327-329.
[3]	李英 . 瓜类蔓枯病菌的生物学特性和黄瓜抗病资源的筛选[D]. 南京: 南京农业大学, 2007.
	LI Y . Study on biology characteristics of Didymella bryoniae and screening of resistance germplasm of cucumber[D]. Nanjing: Nanjing Agricultural University, 2007. (in Chinese)
[4]	孙元超 . 怎样防治黄瓜蔓枯病. 现代农村科技, 2010(17):24-25.
	SUN Y C . How to control gummy stem blight in cucumber. Modern Agricultural Science and Technology, 2010(17):24-25. (in Chinese)
[5]	吴建寨, 韩书庆 . 黄瓜2016年市场分析及2017年市场预测. .
	WU J Z, HAN S Q. Market analysis of cucumber in 2016 and market forecast in 2017. (in Chinese)
[6]	SHARMA V, SALWAN R, SHARMA P N, KANWAR S S . Elucidation of biocontrol mechanisms of Trichoderma harzianum against different plant fungal pathogens: Universal yet host specific response. International Journal of Biological Macromolecules, 2017,95:72-79.
[7]	殷洁, 袁玲 . 寡雄腐霉菌剂对辣椒疫病的防治及促生效应. 园艺学报, 2017,44(12):2327-2337.
	YIN J, YUAN L . Phytophthora disease control and growth promotion of pepper by Pythium oligandrum. Acta Horticulturae Sinica, 2017,44(12):2327-2337. (in Chinese)
[8]	GHOLAMI A, SHAHSAVANI S, NEZARAT S . The effect of plant growth promoting Rhizobacteria(PGPR) on germination, seedling growth and yield of maize. Proceedings of World Academy of Science: Engineering and Technology, 2009,49(1):19-24.
[9]	ARSENEAULT T, GOYER C, FILION M . Pseudomonas fluorescens LBUM223 increases potato yield and reduces common scab symptoms in the field. Phytopathology, 2015,105(10):1311-1317.
[10]	彭于发 . 荧光假单胞菌Tn5诱变防病增产研究初报. 中国农业科学, 1990,23(1):88-89.
	PENG Y F . Effects of Pseudomonas fluorescens mutated by Tn5 on disease suppression and crop yield increase. Scientia Agricultura Sinica, 1990,23(1):88-89. (in Chinese)
[11]	SOWMYA D S, RAO M S, KUMAR R M, GAVASKAR J, PRITI K . Bio-management of Meloidogyne incognita and Erwinia carotovora in carrot(Daucus carota L.) using Pseudomonas putida and Paecilomyces lilacinus. Nematologia Mediterranea, 2012,40:189-194.
[12]	段佳丽, 薛泉宏, 舒志明, 王东胜, 何斐 . 放线菌Act12与腐植酸钾配施对丹参生长及其根域微生态的影响. 生态学报, 2015,35(6):1807-1819. doi: 10.5846/stxb201305231154
	DUAN J L, XUE Q H, SHU Z M, WANG D S, HE F . Effects of combined application of actinomycetes Act12 bio-control agents and potassium humate on growth and microbial flora in rooting zone of Salvia miltiorrhiza Bge. Acta Ecologica Sinica, 2015,35(6):1807-1819. (in Chinese) doi: 10.5846/stxb201305231154
[13]	JANG Y, CHOI H E, LIM Y W, LEE J S, KIM J J . The first report ofCeriporia lacerate(Phanerochaetaceae, Basidiomycota) in Korea. Mycotaxon, 2012,119(1):397-403.
[14]	YUAN H S . A new species of Junghuhnia(Basidiomycota, Meruliaceae) from tropical China. Mycotaxon, 2011,117(1):255-260.
[15]	贾碧丝 . 中国蜡孔菌属分类与系统发育研究[D]. 北京: 北京林业大学, 2012.
	JIA B S . Taxonomy and phylogeny of Ceriporia in China[D]. Beijing: Beijing Forestry University, 2012. (in Chinese)
[16]	JIA B S, ZHOU L W, CUI B K, RIVOIRE B, DAI Y C . Taxonomy and phylogeny of Ceriporia(Polyporales, Basidiomycota) with an emphasis of Chinese collections. Mycological Progress, 2014,13(1):81-93.
[17]	SHIN E J, KIM J E, KIM J H, PARK Y M, YOON S K, JANG B C, LEE S P, KIM B C . Hypoglycemic effects of submerged culture of Ceriporia lacerata mycelium. Korean Journal of Food Preservation, 2015,22(1):145-153.
[18]	王娜, 于圣, 褚衍亮, 徐翔宇, 林陈强 . 撕裂蜡孔菌在开放体系中对甲基橙染料的静态脱色研究. 菌物学报, 2015,34(6):1196-1204.
	WANG N, YU S, CHU Y L, XU X Y, LIN C Q . Decolorization of methyl orange dye by Ceriporia lacerata under statically air-opened condition. Mycosystema, 2015,34(6):1196-1204. (in Chinese)
[19]	WANG N, CHU Y L, WU F, ZHAO Z L, XU X Y . Decolorization and degradation of Congo red by a newly isolated white rot fungus,Ceriporia lacerata, from decayed mulberry branches. International Biodeterioration and Biodegradation, 2017,117:236-244.
[20]	黄建国, 殷洁, 袁玲 . 一株撕裂蜡孔菌及其防治作物真菌病害的应用: CN 107201317 A[P]. ( 2017-09-26) [2018-12-25].
	HUANG J G, YIN J, YUAN L . Disease control by application of a strain of Ceriporia lacerate: CN 107201317 A[P]. ( 2017-09-26) [2018-12-25]. (in Chinese)
[21]	袁玲, 殷洁, 黄建国 . 一株撕裂蜡孔菌的促生作用及应用: CN 107164245 A[P]. ( 2017 -09-15) [2018-12-25].
	YUAN L, YIN J HUANG J G, . Growth promotion by application of a strain of Ceriporia lacerate: CN 107164245 A[P]. (2017 -09-15) [2018-12-25]. (in Chinese)
[22]	殷洁, 范倩, 黄建国 . 撕裂蜡孔菌的新功能——防治茄子绵疫病及促生效应. 中国农业科学, 2018,51(12):2300-2310.
	YIN J, FAN Q, HUANG J G . New functions of Ceriporia lacerate in phytophthora blight control and growth promotion of eggplants. Scientia Agricultura Sinica, 2018,51(12):2300-2310. (in Chinese)
[23]	DIK A J, KONING G, KÖHL J . Evaluation of microbial antagonists for biological control of Botrytis cinerea stem infection in cucumber and tomato. European Journal of Plant Pathology, 1999,105(2):115-122.
[24]	何金环, 连艳鲜 . 生物化学实验技术. 2版. 北京: 中国轻工业出版社, 2014: 156-158.
	HE J H, LIAN Y X. Biochemistry Experiment and Technology. 2nd ed. Beijing: China Light Industry Press, 2014: 156-158. (in Chinese)
[25]	杨剑虹, 王成林, 代亨林 . 土壤农化分析与环境监测. 北京: 中国大地出版社, 2008: 282-287.
	YANG J H, WANG C L, DAI H L. Soil Chemical Analysis and Environmental Monitoring . Beijing: China Earth Press, 2008: 282-287. (in Chinese)
[26]	关松荫 . 土壤酶及其研究法. 北京: 农业出版社, 1986: 274-339.
	GUAN S Y. Soil Enzymes and Its Research Methods. Beijing: Agriculture Press, 1986: 274-339. (in Chinese)
[27]	AGROPAGES . Global pesticide market - by regions and vendors - market size, demand forecasts, industry trends and updates, supplier market shares 2014-2020. .
[28]	贾振华, 李静, 贾中雄 . 化学农药在我国农作物生产中的应用与分析//陈万全. 病虫害绿色防控与农产品质量安全——中国植物保护学会2015年学术年会论文集. 北京: 中国农业科学技术出版社, 2015: 319-322.
	JIA Z H, LI J, JIA Z X. Application and analysis of chemical pesticides in crop production in China//CHEN W Q. Green Prevention and Control of Diseases and Insect Pests and Quality Safety of Agricultural Products——Proceedings of the 2015 Annual Convention of China Society of Plant Protection. Beijing: China Agricultural Science and Technology Press, 2015: 319-322. (in Chinese)
[29]	雷仲仁 . 病虫害生物防治是实现蔬菜安全生产的主要途径. 中国农业科学, 2016,49(15):2932-2934.
	LEI Z R . Biological control of diseases and insect pests is valid method to ensure vegetable safe producing. Scientia Agricultura Sinica, 2016,49(15):2932-2934. (in Chinese)
[30]	O’BRIEN P A . Biological control of plant diseases. Australasian Plant Pathology, 2017,46:293-304.
[31]	BROGLIE K, CHET I, HOLLIDAY M, CRESSMAN R, BIDDLE P, KNOWLTON S, MAUVAIS C J, BROGLIE R . Transgenic plants with enhanced resistance to the fungal pathogen Rhizoctonia solani. Science, 1991,254(5035):1194-1197.
[32]	PICARD K, TIRILLY Y, BENHAMOU N . Cytological effects of cellulases in the parasitism of Phytophthora parasitica by Pythium oligandrum. Applied and Environmental Microbiology, 2000,66(10):4305-4314.
[33]	MAUCH F, MAUCH-MANI B, BOLLER T . Antifungal hydrolases in pea tissue: Ⅱ. Inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase. Plant Physiology, 1988,88(3):936-942.
[34]	GEREMIA R A, GOLDMAN G H, JACOBS D, ARDRTES W, VILA S B, VAN MONTAGU M, HERRERA-ESTRELLA A . Molecular characterization of the proteinase-encoding gene,prb1, related to mycoparasitism by Trichoderma harzianum. Molecular Microbiology, 1993,8(3):603-613.
[35]	管炜, 李淑菊, 王惠哲, 杨瑞环 . 几种杀菌剂对黄瓜蔓枯病菌的室内毒力测定. 天津农业科学, 2010,16(3):82-83.
	GUAN W, LI S J, WANG H Z, YANG R H . Toxicity of some fungicides to Mycosphaerella melonis. Tianjin Agricultural Sciences, 2010,16(3):82-83. (in Chinese)
[36]	宋锐, 林丽果, 王康英, 宋浩然, 蒋勇斌, 刘慧霞 . 不同盐生境下硅对高羊茅生物量及生理生化特征的影响. 草业学报, 2016,25(8):91-97. doi: 10.11686/cyxb2015501
	SONG R, LIN L G, WANG K Y, SONG H R, JIANG Y B, LIU H X . Effects of silicon supply on the biomass and physiochemical features of tall fescue seedlings under different salinization conditions. Acta Prataculturae Sinica, 2016,25(8):91-97. (in Chinese) doi: 10.11686/cyxb2015501
[37]	李书田, 金继运 . 中国不同区域农田养分输入、输出与平衡. 中国农业科学, 2011,44(20):4207-4229. doi: 10.3864/j.issn.0578-1752.2011.20.009
	LI S T, JIN J Y . Characteristics of nutrient input/output and nutrient balance in different regions of China. Scientia Agricultura Sinica, 2011,44(20):4207-4229. (in Chinese) doi: 10.3864/j.issn.0578-1752.2011.20.009
[38]	王克安 . 设施蔬菜高效施肥与土壤无害化处理. 北京: 金盾出版社, 2015.
	WANG K A. High Efficiency Fertilization for Vegetable Plants and Harmlessniss Treatment of Soil. Beijing: Jindun Publishing House, 2015. (in Chinese)
[39]	JOHNSON S E, LOEPPERT R H . Role of organic acids in phosphate mobilization from iron oxide. Soil Science Society of America Journal, 2006,70(1):222-234.
[40]	刘丽, 梁成华, 王琦, 杜立宇, 吴玉梅, 韩巍 . 低分子量有机酸对土壤磷活化影响的研究. 植物营养与肥料学报, 2009,15(3):593-600. doi: 10.11674/zwyf.2009.0315
	LIU L, LIANG C H, WANG Q, DU L Y, WU Y M, HAN W . Effects of low-molecular-weight organic acids on soil phosphorus release. Plant Nutrition and Fertilizer Science, 2009,15(3):593-600. (in Chinese) doi: 10.11674/zwyf.2009.0315
[41]	王东升, 王君 . 低分子量有机酸作用下土壤矿物钾释放机制. 辽宁工程技术大学学报(自然科学版), 2009,28(Suppl. 2):259-261.
	WANG D S, WANG J . Mechanism of soil mineral potassium release extracted by low-molecular-weigh organic acids. Journal of Liaoning Technical University (Natural Science), 2009,28(Suppl. 2):259-261. (in Chinese)
[42]	杨扬, 高克祥, 吴岩, 刘晓光 . 吲哚乙酸跨界信号调节植物与细菌互作. 生物技术通报, 2016,32(8):14-21. doi: 10.13560/j.cnki.biotech.bull.1985.2016.08.003
	YANG Y, GAO K X, WU Y, LIU X G . Indole-3-acetic acid-mediated cross-kingdom signalling involved in plant-bacteria interactions. Biotechnology Bulletin, 2016,32(8):14-21. (in Chinese) doi: 10.13560/j.cnki.biotech.bull.1985.2016.08.003
[43]	KLOEPPER J W, LEONG J, TEINTZE M, SCHROTH M N . Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria. Nature, 1980,286(2):885-886.
[44]	徐凯, 郭延平, 张上隆 . 不同光质对草莓叶片光合作用和叶绿素荧光的影响. 中国农业科学, 2005,38(2):369-375.
	XU K, GUO Y P, ZHANG S L . Effect of light quality on photosynthesis and chlorophyll fluorescence in strawberry leaves. Scientia Agricultura Sinica, 2005,38(2):369-375. (in Chinese)
[45]	孙洪助 . 红蓝光比例对绿叶蔬菜生理特性及品质的影响[D]. 南京: 南京农业大学, 2014.
	SUN H Z . Effects of proportions of red and blue light on physiological characteristics and quality in leafy greens[D]. Nanjing: Nanjing Agricultural University, 2014. (in Chinese)
[46]	王素平, 郭世荣, 李璟, 胡晓辉, 焦彦生 . 盐胁迫对黄瓜幼苗根系生长和水分利用的影响. 应用生态学报, 2006,17(10):1883-1888.
	WANG S P, GUO S R, LI J, HU X H, JIAO Y S . Effects of salt stress on the root growth and leaf water use efficiency of cucumber seedlings. Chinese Journal of Applied Ecology, 2006,17(10):1883-1888. (in Chinese)
[47]	张丽娟, 曲继松, 朱倩楠, 吴涛 . 不同剂量外源纤维素酶对设施土壤生物活性与番茄生长的影响. 植物营养与肥料学报, 2017,23(4):1089-1094.
	ZHANG L J, QU J S, ZHU Q N, WU T . Effects of exogenous cellulase with different dosages on the biological activity and tomato growth in greenhouse soil. Journal of Plant Nutrition and Fertilizer, 2017,23(4):1089-1094. (in Chinese)
[48]	杨丽娟, 须晖, 邱忠祥, 刘永青 . 菜田土壤酶活性与黄瓜产量的关系. 植物营养与肥料学报, 2000,6(1):113-116. doi: 10.11674/zwyf.2000.0117
	YANG L J, XU H, QIU Z X, LIU Y Q . Relationship between activities of enzyme and cucumber yield in vegetable soil. Plant Nutrition and Fertilizer Science, 2000,6(1):113-116. (in Chinese) doi: 10.11674/zwyf.2000.0117
[49]	DE LA PAZ JIMENEZ M, DE LA HORRA A, PMZZO L, PALMA R M . Soil quality: a new index based on microbiological and biochemical parameters. Biology and Fertility of Soils, 2002,35:302-306.
[50]	申卫收, 林先贵, 张华勇, 尹睿, 段增强, 施卫明 . 不同施肥处理下蔬菜塑料大棚土壤微生物活性及功能多样性. 生态学报, 2008,28(6):2682-2689.
	SHEN W S, LIN X G, ZHANG H Y, YIN R, DUAN Z Q, SHI W M . Microbial activity and functional diversity in soils used for the commercial production of cucumbers and tomatoes in polytunnel greenhouse, under different fertilization. Acta Ecologica Sinica, 2008,28(6):2682-2689. (in Chinese)

处理 Treatment	病情指数 Disease index	发病率 Incidence (%)	防治效果 Control efficacy (%)	丙二醛含量 Malondialdehyde content (μmol·g^-1)	相对电导率 Relative electric conductivity (%)
CK	0c	0c	—	3.95±0.03d	100.00±0.58c
PI	38.40±1.11a	36.67±3.33a	0b	8.14±1.37a	148.55±3.79a
TM+PI	9.38±1.20b	10.00±0b	75.57±3.11a	5.47±0.26b	129.48±5.57b
CLB+PI	7.80±0.92b	10.00±3.33b	79.69±2.40a	5.03±0.33c	128.32±6.09b

处理 Treatment	含量 Content (%)			吸收量 Absorption (g/plant)
处理 Treatment	氮N	磷P	钾K	氮N	磷P	钾K
CK	1.36±0.06b	1.34±0.01c	4.86±0.09c	0.30±0.05d	0.30±0.04d	1.10±0.12d
CF	1.46±0.03b	1.35±0.03c	5.57±0.21c	0.42±0.03c	0.39±0.03c	1.61±0.10c
CF+CLB₇₅	1.88±0.10a	1.46±0.04b	5.95±0.12b	0.61±0.03b	0.47±0.06b	1.93±0.25b
CF+CLB₁₅₀	1.94±0.07a	1.52±0.03a	6.27±0.05a	0.71±0.03a	0.56±0.01a	2.23±0.17a

处理 Treatment	单果重 Fruit weight (g)	单株结果数 Fruit quantity of plant	产量 Yield (kg·667 m^-2)	可溶性糖 Soluble sugar (%)	维生素C Vitamin C (mg·100 g^-1)	可溶性蛋白 Soluble protein (mg·100 g^-1)	游离氨基酸 Free amino acid (mg·100 g^-1)	硝酸盐 Nitrate (μg·g^-1)
CK	210.42±14.76b	3.68±0.17c	2709.58±172.18c	2.30±0.08a	7.40±0.46b	98.32±5.88a	54.16±5.12b	59.76±2.24c
CF	241.77±11.21a	6.17±0.13b	5216.28±210.63b	2.66±0.30a	8.29±0.34a	101.57±8.23a	52.36±2.21b	80.20±6.64a
CF+CLA₁₀	241.58±6.98a	7.01±0.29a	5939.75±412.63a	2.69±0.07a	8.59±0.64a	100.60±9.58a	89.82±4.24a	75.11±2.01ab
75% CF+CLA₁₀	233.86±16.79a	6.88±0.31a	5826.92±411.05a	2.66±0.34a	8.71±0.32a	103.77±11.63a	80.83±11.58a	68.23±6.79bc