中国农业科学 ›› 2016, Vol. 49 ›› Issue (13): 2524-2533.doi: 10.3864/j.issn.0578-1752.2016.13.008

所属专题: 烟粉虱

• 植物保护 • 上一篇    下一篇

番茄不同抗性品种响应B型烟粉虱胁迫的生理特性

蔡冲,徐盈盈,崔旭红   

  1. 中国计量大学生命科学学院/浙江省生物计量及检验检疫技术重点实验室,杭州 310018
  • 收稿日期:2015-11-04 出版日期:2016-07-01 发布日期:2016-07-01
  • 通讯作者: 崔旭红,Tel:0571-86835774;Fax:0571-86914449;E-mail:xuhongcui@cjlu.edu.cn
  • 作者简介:蔡冲,Tel:0571-86835774;Fax:0571-86914449;E-mail:ccjacn@cjlu.edu.cn
  • 基金资助:
    国家自然科学基金(31401923)、浙江省自然科学基金(LQ12C14005)

Analysis of Physiological Characteristics with Response to Bemisia tabaci B Biotype in Different Resistant Varieties of Tomato

CAI Chong, XU Ying-ying, CUI Xu-hong   

  1. College of Life Sciences, China Jiliang University/Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, Hangzhou 310018
  • Received:2015-11-04 Online:2016-07-01 Published:2016-07-01

摘要: 【目的】明确在B型烟粉虱(Bemisia tabaci)胁迫下,番茄抗虫品种和感虫品种叶片的生理特性变化,为生产上选育和推广番茄抗烟粉虱品种提供参考。【方法】在人工气候室内,以番茄抗虫品种红圣女(Hongshengnv,HSN)和感虫品种黄圣果(Huangshengguo,HSG)为研究材料,通过非选择性试验,比较研究在B型烟粉虱胁迫下,叶片的次生代谢物质(蔗糖酯、没食子酸、咖啡酸、阿魏酸、苯甲酸、水杨酸、木质素)含量、防御酶(过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)、脂氧合酶(LOX))活性、胰蛋白酶抑制剂(TI)活性、光合参数值(Fv/Fm、Fv/Fo)、活性氧(H2O2含量、 产生速率)、丙二醛(MDA)含量、细胞膜电解质渗出率(EC)的变化。【结果】红圣女与黄圣果的蔗糖酯、酚酸、木质素、H2O2和丙二醛含量以及Fv/Fo值、防御酶和胰蛋白酶抑制剂活性均存在显著差异(P<0.05);而两个品种的Fv/Fm、  产生速率、细胞膜电解质渗出率没有显著差异(P>0.05)。两个品种对B型烟粉虱胁迫的响应差异明显,胁迫9 h后,叶片的蔗糖酯、酚酸、木质素含量和防御酶活性均升高,且红圣女的升高程度强于黄圣果;叶片发生光抑制现象,光合参数值和胰蛋白酶抑制剂活性均下降,红圣女的下降程度小于黄圣果;同时叶片产生大量活性氧,丙二醛积累、细胞膜电解质渗出率上升,黄圣果的细胞受损伤程度高于红圣女。【结论】番茄不同抗性品种对B型烟粉虱胁迫的响应存在差异性,抗虫品种响应次生代谢物质含量和防御酶活性的变化更强烈;感虫品种响应光合参数、活性氧、细胞膜脂过氧化和胰蛋白酶抑制剂活性的变化更强烈。

关键词: 番茄, 抗性品种, B型烟粉虱, 胁迫, 生理特性

Abstract: 【Objective】The objective of this study is to clarify the physiological characteristic changes inside the leaves of tomato between the resistance and the susceptible cultivars under the stresses of Bemisia tabaci B biotype, and to provide a reference for the breeding and popularization of tomato varieties resistant to B. tabaci.【Method】 An experiment was carried out under artificial climate conditions. The resistant variety (Hongshengnv, HSN) and the susceptible variety (Huangshengguo, HSG) were infested by B. tabaci B biotype in a no-choice way. The contents variation of the secondary metabolic materials (i.e., sucrose esters, gallic acid, caffeic acid, ferulic acid, benzoic acid, salicylic acid, lignin), the changes of some protective enzymes activities (i.e., POD, CAT, PPO, LOX) and trypsin proteinase inhibitor activity (TI), the value variation of two photosynthetic parameters (i.e., Fv/Fm, Fv/Fo), the changes of cell membrane lipid peroxidation (i.e. H2O2 content, production rate, MDA content and electric conductivity (EC)), were determined all together at the same time.【Result】There were marked differences in the contents of the SE, phenolic acids, lignin, H2O2, and MDA between the HSN and the HSG. Meanwhile, the differences of the activities of TI and protective enzymes, the values of Fv/Fo, were also significant between these two varieties. However, the value differences of these parameters, such as Fv/Fm, production rate, and EC, were not obvious. The two varieties showed different responses to the infestation of B. tabaci B biotype. For example, after 9 h, the contents of sucrose esters, phenolic acids, and lignin as well as the activities of protective enzymes increased all together. However, there were considerable raises as to its extent with comparing HSN to the HSG. Photosynthesis was inhibited in the leaves, confirmed by the observation of reducing of the activities of TI and photosynthetic parameter values. There were reducing to lower intensities with comparing the HSN to the HSG. More active oxygen, MDA and EC found in the latter indicated that HSG cell had been damaged greatly.【Conclusion】There were different responses to the stresses of B. tabaci B biotype as to tomato of different resistant varieties. Higher variation of secondary metabolic materials contents and protective enzymes activities were observed in the resistant variety. Meanwhile, the photosynthetic parameter values, active oxygen content, cell membrane lipid peroxidation, as well as the TI activities changed more dramatically in the susceptible variety.

Key words: Lycopersicon esculentum, resistant varieties, Bemisia tabaci B biotype, stress, physiological characteristics