根系边缘细胞对肉桂酸胁迫下黄瓜和黑籽南瓜活性氧代谢与根系活力的影响

doi:10.3864/j.issn.0578-1752.2015.08.12

摘要/Abstract

摘要： 【目的】了解肉桂酸（CA）胁迫下根系边缘细胞（RBC）对黄瓜和黑籽南瓜活性氧代谢与根系活力的影响，探索RBC增强根尖抵御CA胁迫的生理机制，阐明黑籽南瓜抵御CA胁迫能力强于黄瓜的原因。【方法】选用‘中农16’黄瓜和云南黑籽南瓜种子，采用悬空培养的方法，待根系长到5 mm时分成两组。组1：每隔1 h对黄瓜和黑籽南瓜的初生根分别喷施0和0.25 mmol·L^-1的CA水溶液（pH 6.0）；组2：种子的初生根先用小水流（蒸馏水）每4 h冲洗一次，去除RBC，再用0和0.25 m mol·L^-1的CA水溶液每1 h喷施根系1次。处理0、12、24和36 h时测量活性氧代谢指标；处理24 h时测量根系鲜重、根系呼吸速率和根系活力等指标。【结果】无CA胁迫时，有无RBC对根尖的鲜重和生理代谢没有显著影响。当根尖附着RBC时，CA胁迫导致黄瓜根系鲜重和根系活力下降，活性氧（ROS）水平和丙二醛（MDA）含量显著增加，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）及过氧化物酶（POD）的活性明显上升，呼吸速率和抗CN呼吸速率也明显上升；与黄瓜明显不同的是，CA胁迫对黑籽南瓜根系鲜重、根系活力、ROS水平、MDA含量、SOD、CAT及POD的活性、呼吸速率和抗CN呼吸速率影响均不显著。RBC去除后，CA胁迫对黄瓜根系的生理代谢影响趋势和RBC去除之前相一致，胁迫程度更大；黑籽南瓜受到CA胁迫的影响与黄瓜基本一致，较RBC去除前差异显著。【结论】RBC通过降低根系中ROS水平及MDA含量，减轻了CA对黄瓜和黑籽南瓜根系的毒害；黑籽南瓜的RBC防御CA对自身根系毒害的能力明显强于黄瓜；若根尖没有RBC保护，CA对黄瓜和黑籽南瓜均造成明显的毒害。

关键词: 黄瓜, 黑籽南瓜, 肉桂酸, 根系边缘细胞, 活性氧

Abstract: 【Objective】The aim of this paper was to clarify the defensive mechanism of root border cells (RBC) against cinnamic acid (CA) stress by analyzing the reactive oxygen metabolism and root activity of seedling RBC in cucumber (Cucumis sativus L.) and figleaf gourd(Cururbita ficifolia) under CA stress. 【Method】 The 5 mm length of aeroponicly cultured roots of cucumber cultivar Zhongnong No. 16 andfigleaf gourd cultivar bouche were divided into two groups: one group was used to investigate reactive oxygen metabolism by spraying with 0 and 0.25 mmol·L^-1 CA at 1 h interval, another group rinsing root tips of distilled water once every 4 h firstly and RBC were removed, then spraying 0 and 0.25 mmol·L^-1 CA every 1 h. The metabolic index of active oxygen was measured after treatment for 0, 12, 24 and 36 hours of CA stress, root fresh weight, root respiration rate and root activity of the seedlings were measured after 24 hours. 【Result】The root fresh weight and physiological metabolism were not affected significantly by RBC without CA stress. If the RBC were not removed, the root fresh weight and root activity decreased and the level of reactive oxygen species (ROS) and malonaldehyde (MDA), total respiration rate, CN-resistant respiration rate, the activities of superoxide dismutase (SOD), catalase (CAT) and guaiacol-dependent peroxidase (POD) increased significantly in cucumber under CA stress. However, the root fresh weight, root activity, the level of ROS and MDA, total respiration rate, CN-resistant respiration rate, the activities of SOD, CAT and POD were not affected significantly in figleaf gourd seedlings. If RBC were removed, the effect of CA stress on figleaf gourd was similar with cucumber, but significantly than RBC not removing. 【Conclusion】 RBC possibly attenuated the CA toxicity to the roots of cucumber and figleaf gourd through decreasing ROS and MDA content. The defensive ability of RBCs against CA on figleaf gourd was stronger than that of cucumber. Removal of RBCs could lead to great damage to the roots of cucumber and figleaf gourd significantly under CA stress.

Key words: cucumber, figleaf gourd, cinnamic acid, root border cells, reactive oxygen species

乔永旭，张永平，高丽红. 根系边缘细胞对肉桂酸胁迫下黄瓜和黑籽南瓜活性氧代谢与根系活力的影响[J]. 中国农业科学, 2015, 48(8): 1579-1587.

QIAO Yong-xu, ZHANG Yong-ping, GAO Li-hong. Effect of Root Border Cells on Reactive Oxygen Metabolism and Root Activity of Cucumber and Figleaf Gourd Seedlings Under Cinnamic Acid Stress[J]. Scientia Agricultura Sinica, 2015, 48(8): 1579-1587.

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